A5043707722- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Stress Responses and Cortisol
- Biochemical Analysis and Sensing Techniques
- Memory and Neural Mechanisms
- Regulation of Appetite and Obesity
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Nicotinic Acetylcholine Receptors Study
- Olfactory and Sensory Function Studies
- Neuroscience and Neuropharmacology Research
- Neurological Disorders and Treatments
- Vascular Tumors and Angiosarcomas
- Monoclonal and Polyclonal Antibodies Research
- Birth, Development, and Health
- Cutaneous lymphoproliferative disorders research
- Wnt/β-catenin signaling in development and cancer
Universidad Agraria del Ecuador
2025
University of Pennsylvania
2017-2021
Philadelphia University
2018
California University of Pennsylvania
2018
Stanford University
2012-2016
Howard Hughes Medical Institute
2014
Emory University
2011-2013
University of Virginia
1996
Instituto de Investigación Médica Mercedes y Martín Ferreyra
1986-1988
Single-cell RNA sequencing (scRNA-seq) enables the quantification of each gene's expression distribution across cells, thus allowing assessment dispersion, nonzero fraction, and other aspects its beyond mean. These statistical characterizations gene are critical for understanding variation selecting marker genes population heterogeneity. However, scRNA-seq data noisy, with cell typically sequenced at low coverage, making it difficult to infer properties from raw counts. Based on a...
We developed a platform to isolate brain-derived extracellular vesicles and measure their miRNA biomarkers classify specific states of traumatic brain injury.
The research was conducted in an open field at the Villaforest farm, Chongón parish, Guayas Province. genus Anastrepha spp. is one of pests considered quarantine for other countries worldwide and most prevalent South America. This aimed to find effective low-cost lure that helps reduce fruit fly populations. experimental design used a randomized complete block (RCBD), means comparisons were made with non-parametric Friedman test. treatments evaluated from blossom fruiting stages mangoes....
Cytoplasmic dynein is the microtubule minus-end-directed motor for retrograde axonal transport of membranous organelles. Because its similarity to intermediate chains flagellar dynein, 74-kDa chain (IC74) subunit thought be involved in binding organelle cargo. Previously, we identified six isoforms IC74 cytoplasmic brain. We further demonstrated that cultured glia and neurons expressed different phospho-isoforms. Two were observed when from was analyzed. When analyzed, observed, although...
Abstract Rapid advances in massively parallel single cell RNA sequencing (scRNA-seq) is paving the way for high-resolution profiling of biological samples. In most scRNA-seq studies, only a small fraction transcripts present each are sequenced. The efficiency, that is, proportion sequenced, can be especially low highly parallelized experiments where number reads allocated small. This leads to unreliable quantification lowly and moderately expressed genes, resulting extremely sparse data...
Embryonic dermal fibroblasts in the skin have exceptional ability to initiate hair follicle morphogenesis and contribute scarless wound healing. Activation of Wnt signaling pathway is critical for fibroblast fate selection induction. In humans, mutations components target genes lead congenital focal hypoplasias with diminished hair. The gene expression signature embryonic during differentiation its dependence on unknown. Here we applied Shannon entropy analysis identify mouse fibroblasts. We...
Insulators are DNA sequences thought to be important for the establishment and maintenance of cell-type specific nuclear architecture. In Drosophila there several classes insulators that appear have unique roles in gene expression. The mechanisms involved determining regulating these insulator not understood. Here we report Topoisomerase II modulates activity Su(Hw) insulator. Downregulation Topo by RNAi or mutations Top2 result disruption function. This effect is mediated Mod(mdg4)2.2...
While much is known about genes that promote aging, little protect against or prevent particularly in human skin. The main objective of this study was to perform an unbiased, whole transcriptome search for associate with intrinsic skin youthfulness. To accomplish this, healthy women (n = 122) European descent, ages 18-89 years Fitzpatrick type I/II were examined facial aging parameters and clinical covariates, including smoking ultraviolet exposure. Skin youthfulness defined as the top 10%...
Abstract Non-genetic factors can cause individual cells to fluctuate substantially in gene expression levels over time. Yet it remains unclear whether these fluctuations persist for much longer than the time of one cell division. Current methods measuring single mostly rely on point measurements, making duration or cellular memory difficult measure. Here, we report a method combining Luria and Delbrück’s fluctuation analysis with population-based RNA sequencing (MemorySeq) identifying genes...
Background Strains from a collection of Drosophila GFP protein trap lines express in the normal tissues where endogenous is present. This can be used to screen for proteins distributed nucleus non-uniform pattern. Methodology/Principal Findings We analyzed four that show peripheral or punctate nuclear staining. One these affects an uncharacterized gene named CG11138. The CG11138 shows distribution periphery similar insulator but does not co-localize with known insulators. Interestingly,...