A5012082696- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Virus-based gene therapy research
- Platelet Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Chronic Lymphocytic Leukemia Research
- vaccines and immunoinformatics approaches
- Biotin and Related Studies
- Bacteriophages and microbial interactions
- T-cell and B-cell Immunology
- Pancreatic function and diabetes
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Erythrocyte Function and Pathophysiology
- Cancer Immunotherapy and Biomarkers
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Folate and B Vitamins Research
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- Growth Hormone and Insulin-like Growth Factors
- RNA Research and Splicing
Enzo Life Sciences (United States)
2023
New England Biolabs (United States)
2021
BioNTech (United States)
2020
Juno Therapeutics (Germany)
2017
The DNAs of bacterial viruses are known to contain diverse, chemically complex modifications thymidine that protect them from the endonuclease-based defenses their cellular hosts, but whose biosynthetic origins enigmatic. Up half thymidines in Pseudomonas phage M6, Salmonella ViI, and others, exotic chemical moieties synthesized through post-replicative modification 5-hydroxymethyluridine (5-hmdU). We have determined these hypermodifications derived free amino acids enzymatically installed...
Abstract Insulin-like growth factor (IGF) signaling is highly conserved and tightly regulated by proteases including Pregnancy-Associated Plasma Protein A (PAPP-A). PAPP-A its paralog PAPP-A2 are metalloproteases that mediate IGF bioavailability through cleavage of binding proteins (IGFBPs). Here, we present single-particle cryo-EM structures the catalytically inactive mutant (E483A) in complex with a peptide from substrate IGFBP5 (PAPP-A BP5 ) also substrate-free form, leveraging power...
Myeloproliferative leukemia protein (MPL), also known as thrombopoietin (TPO) receptor, is a class I cytokine receptor that expressed on hematopoietic progenitors, promoting growth and differentiation toward the megakaryocyte lineage critical for normal platelet production. Mutations in MPL, TPO, or Janus kinase 2 (JAK2) have been implicated multiple diseases from congenital thrombocytopenias to myeloproliferative neoplasms. The ligand stimulates production by inducing MPL dimerization...
Abstract Pregnancy-Associated Plasma Protein A isoforms, PAPP-A and PAPP-A2, are metalloproteases that cleave insulin-like growth factor binding proteins (IGFBPs) to modulate signaling. The structures of homodimeric in complex with IGFBP5 anchor peptide, inhibitor STC2 proMBP have been recently reported. Here, we present the single-particle cryo-EM structure monomeric, N-terminal LG, MP, M1 domains (with exception LNR1/2) human PAPP-A2 3.13 Å resolution. Our together functional studies...
<h3>Background</h3> Dendtritic cell (DC) vaccine therapies have so far demonstrated limited success as cancer therapeutics. Recently, cDC1 been shown to support CD8 and CD4 activation, both types were necessary for anti-tumor responses.<sup>1 2</sup> <h3>Methods</h3> To test the ability of promote these responses, we designed produced a stabilized Xcl1 protein molecule fused Fc-chicken ovalbumin (OVA) model antigen that specifically engages activate by targeting Xcr1-Xcl1 interaction....
Abstract eIF2B is a decameric guanine nucleotide exchange factor (GEF) that essential for protein synthesis and key effector of the integrated stress response (ISR). Hypomorphic mutations in any subunits are associated with Vanishing White Matter Disease (VWM), leukodystrophy characterized by ISR activation white matter loss. Here, we showed VWM-associated N208Y eIF2Bα mutation, which abolishes sugar phosphate binding, led to drastic reduction its level cells concomitant activation. We found...
<h3>Background</h3> Neoantigens are tumor-specific antigens that important in the anti-tumor immune response. These not subject to central tolerance and therefore potentially more immunogenic than tumor-associated antigens. NEO-STIM<sup>®</sup>, our propriety <i>ex vivo</i> induction process, was developed generate T-cell products specific these neoantigens from peripheral blood of patient. Here, we present results a proof concept, pre-clinical study with multiple successful process...
Abstract Background: Neoantigens are tumor-specific antigens that have been shown to be critical in the anti-tumor immune response. These not subject central tolerance and therefore potentially more immunogenic than tumor-associated antigens. The goal of our studies is assess potential high-quality neoantigen targets, as defined through bioinformatic engine RECON®, well perform detailed characterization induced T cell responses towards these targets. Methods: Patient-specific neoantigens...
Background: Neoantigens are tumor-specific antigens that have been shown to be critical in the anti-tumor immune response. These not subject central tolerance and therefore potentially more immunogenic than tumor-associated antigens. The goal of our studies is assess potential high-quality neoantigen targets, as defined through bioinformatic engine RECON®, well perform detailed characterization induced T cell responses towards these targets.Methods: Patient-specific neoantigens were...