A5004618507- Osteoarthritis Treatment and Mechanisms
- Lymphoma Diagnosis and Treatment
- Chemokine receptors and signaling
- Cell Adhesion Molecules Research
- CNS Lymphoma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Cancer Genomics and Diagnostics
- Genomics and Phylogenetic Studies
- Total Knee Arthroplasty Outcomes
- Sarcoma Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Chronic Lymphocytic Leukemia Research
- Bone Metabolism and Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Lipid metabolism and disorders
- Sarcoidosis and Beryllium Toxicity Research
- Immune cells in cancer
- Genomics and Rare Diseases
- Multiple Myeloma Research and Treatments
Palacký University Olomouc
2015-2024
University Hospital Olomouc
2019-2024
The insufficient standardisation of diagnostic next-generation sequencing (NGS) still limits its implementation in clinical practice, with the correct detection mutations at low variant allele frequencies (VAF) facing particular challenges. We address here coverage depth order to minimise probability false positive and negative results, latter being underestimated NGS. There is currently no consensus on minimum depth, so each laboratory has set own parameters. To assist laboratories...
Sarcoidosis is an inflammatory granulomatous disease with unknown etiology driven by cytokines and chemokines. There limited information regarding the regulation of cytokine/chemokine‐receptor network in bronchoalveolar lavage (BAL) cells pulmonary sarcoidosis, suggesting contribution miRNAs transcription factors. We therefore investigated gene expression 25 inflammation‐related miRNAs, 27 cytokines/chemokines/receptors, a Th1‐transcription factor T-bet unseparated BAL obtained from 48...
Objective Aseptic loosening (AL) is the most frequent long-term reason for revision of total knee arthroplasty (TKA) affecting about 15–20% patients within 20 years after surgery. Although there a solid body evidence crucial role inflammation in AL pathogenesis, scared information on signature and its time-axis tissues around TKA exists. Design The protein signatures pseudosynovial collected at surgery from with (AL, n = 12) those no clinical/radiographic signs (non-AL, 9) were investigated...
<h3>Background</h3> Systemic lupus erythematosus (SLE) is a severe autoimmune connective tissue disorder. Current knowledge about the serum protein pattern associated with SLE still limited. <h3>Objectives</h3> To identify SLE-associated signature using innovative multiplex Proximity Extension ImmunoAssay (PEA). <h3>Methods</h3> We investigated levels of 92 inflammation-related proteins in 76 Czech patients and 24 age-matched healthy control subjects (C) highly sensitive PEA (Proseek...
Background: Among the most important prognostic genetic aberrations in multiple myeloma (MM) are del(17p) and/or TP53 mutations. The use of whole bone marrow (BM), when sorted MM cells not available, may greatly simplify NGS assessment patients for clinical decisions. Aims: Therefore, objective our study was to assess applicability ultra‐deep targeted analysis mutations and other commonly mutated genes NRAS, KRAS BRAF using BM samples compare it results from cells. Methods: were obtained (n...