A5060690162- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Mosquito-borne diseases and control
- Chromium effects and bioremediation
- Genomic variations and chromosomal abnormalities
- Digestive system and related health
- Systemic Lupus Erythematosus Research
- Osteoarthritis Treatment and Mechanisms
- Chemokine receptors and signaling
- Lymphoma Diagnosis and Treatment
- Heavy Metal Exposure and Toxicity
- Immune cells in cancer
- Immune Response and Inflammation
- Multiple Myeloma Research and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- GDF15 and Related Biomarkers
- Adipose Tissue and Metabolism
- Mass Spectrometry Techniques and Applications
- Reproductive System and Pregnancy
- Genetic factors in colorectal cancer
- HER2/EGFR in Cancer Research
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
Engineering School of Information and Digital Technologies
2025
VSB - Technical University of Ostrava
2020-2024
University Hospital Olomouc
2021-2024
Palacký University Olomouc
2021-2024
Abstract Extramedullary disease (EMM) represents a rare, aggressive and mostly resistant phenotype of multiple myeloma (MM). EMM is frequently associated with high-risk cytogenetics, but their complex genomic architecture largely unexplored. We used whole-genome optical mapping (Saphyr, Bionano Genomics) to analyse the CD138+ cells isolated from bone-marrow aspirates an unselected cohort newly diagnosed patients (n = 4) intramedullary MM 7). Large intrachromosomal rearrangements (> 5 Mbp)...
Aim: In 2005-2006, a chikungunya epidemic of unprecedented magnitude hit Reunion Island, which raised public health concern through the substantial proportions long-lasting manifestations. To understand pathophysiology underlying chronic (CC), we designed CHIKGene cohort study and collected blood samples from 133 subjects diagnosed with CC 86 control individuals that had recovered within 3 months, 12-to-15 years after exposure. Methods: We conducted bulk RNAseq analysis on peripheral...
Abstract Background Long-term environmental exposure to metals leads epigenetic changes and may increase risks human health. The relationship between the type level of metal in subjects exposed high concentrations environment is not yet clear. aim our study find possible association long-term with DNA methylation genes related immune response carcinogenesis. We investigated plasma levels 21 essential non-essential detected by ICP-MS 654 CpG sites located on NFKB1, CDKN2A, ESR1, APOA5 , IGF2...
Plant pathogens are a serious threat to agriculture for long-term viability and cost loss of billions dollars yearly. Many have been documented in the literature that infect variety crops. Nevertheless, new emerge often result disease outbreaks, leading million-dollar losses. Currently, several diagnostic approaches with enhanced sensitivity specificity identification widespread and/or unknown plant constantly being developed. Whereas extensively used pathogen diagnostics mostly serological...
Recent studies have shown the potential of using long-read whole-genome sequencing (WGS) approaches and optical mapping (OM) for detection clinically relevant structural variants (SVs) in cancer research. Three main WGS platforms are currently use: Pacific Biosciences (PacBio), Oxford Nanopore Technologies (ONT) 10x Genomics. Recently, OM technology (Bionano Genomics) has been introduced into human diagnostics. Questions remain about accuracy these platforms, how comparable/interchangeable...
Despite the shared pattern of surface antigens, neoplastic cells in chronic lymphocytic leukemia (CLL) are highly heterogeneous CD5 expression, a marker linked to proliferative pool cells. To further characterize high and low cells, we assessed chemokine receptors (CCR5, CCR7, CCR10, CXCR3, CXCR4, CXCR5) adhesion molecules (CD54, CD62L, CD49d) on subpopulations, defined by CD5/CD19 coexpression, peripheral blood CLL patients (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
The tissue microenvironment in chronic lymphocytic leukaemia (CLL) plays a key role the pathogenesis of CLL, but complex blood CLL has not yet been fully characterised. Therefore, immunophenotyping circulating immune cells 244 patients and 52 healthy controls was performed using flow cytometry analysed by multivariate Patient Similarity Networks (PSNs). Our study revealed high inter-individual heterogeneity distribution activation bystander depending on bulk cells. High counts were...
Glucocorticoids (GCs) are widely used as a treatment for rheumatoid arthritis (RA), leading to high cumulative doses in long-term treated patients. The impact of GC dose on the systemic inflammatory response RA remains poorly understood.
Antiphospholipid syndrome (APS) is associated with recurrent pregnancy morbidity, yet the underlying mechanisms remain elusive. We performed multifaceted characterization of biological and transcriptomic signatures mouse placenta uterine natural killer (uNK) cells in APS. Histological analysis APS placentas unveiled placental abnormalities, including disturbed angiogenesis, occasional necrotic areas, fibrin deposition, nucleated red blood cell enrichment. Analyses showed a reduced...
Chronic lymphocytic leukaemia (CLL) is a genetically, morphologically and phenotypically heterogeneous chronic disease with clinical variability between patients. Whether the significant heterogeneity of cell size within CLL population contributes to features this has not been investigated. The present study aimed characterise phenotypic functional properties two subpopulations typical cells that differ in size: small (s-CLL) large (l-CLL) delineated by forward scatter cytometry. s-CLL were...
Summary Germline pathogenic ATM (ataxia‐telangiectasia mutated) variants are associated with the risk of multiple cancers; however, genetic testing reveals a large number uncertain significance (VUS). Here, we studied germline occurring in real‐world cohort 336 patients chronic lymphocytic leukaemia (CLL) and public cancer whole‐exome/genome‐sequencing datasets (445 CLL, 75 mantle cell lymphoma, 216 metastatic breast cancer, 140 lung patients). We found that two‐thirds rare (18%–50%) or...
Abstract Background: The tissue microenvironment in chronic lymphocytic leukemia (CLL) plays a key role promoting neoplastic cell survival, proliferation, and drug resistance. There is lack of complex characterization CLL blood its clinical impact. Methods: Immunophenotypic profiles circulating immune cells 244 patients (untreated, n=123; novel agents, n=67; previous immunochemotherapy, n=54) age/sex-matched healthy controls (n=52) were assessed using flow cytometry analyzed by multivariate...
Abstract Background: The tissue microenvironment in chronic lymphocytic leukemia (CLL) plays a key role promoting neoplastic cell survival, proliferation, and drug resistance. There is lack of complex characterization CLL blood its clinical impact. Methods: Immunophenotypic profiles circulating immune cells 244 patients (untreated, n=123; novel agents, n=67; previous immunochemotherapy, n=54) age/sex-matched healthy controls (n=52) were assessed using flow cytometry analyzed by multivariate...