A5023239518- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Viral-associated cancers and disorders
- Immunodeficiency and Autoimmune Disorders
- Multiple Myeloma Research and Treatments
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Lung Cancer Treatments and Mutations
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Advanced Breast Cancer Therapies
- CNS Lymphoma Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Medical Imaging Techniques and Applications
- Multiple and Secondary Primary Cancers
- Cancer Genomics and Diagnostics
- Neutropenia and Cancer Infections
- Cutaneous lymphoproliferative disorders research
- Myasthenia Gravis and Thymoma
- CAR-T cell therapy research
- Cancer Treatment and Pharmacology
- T-cell and Retrovirus Studies
- Education, Psychology, and Social Research
Palacký University Olomouc
2016-2025
University Hospital Olomouc
2016-2025
Ocní klinika
1997-2016
Masaryk University
2004
The insufficient standardisation of diagnostic next-generation sequencing (NGS) still limits its implementation in clinical practice, with the correct detection mutations at low variant allele frequencies (VAF) facing particular challenges. We address here coverage depth order to minimise probability false positive and negative results, latter being underestimated NGS. There is currently no consensus on minimum depth, so each laboratory has set own parameters. To assist laboratories...
Chronic lymphocytic leukaemia (CLL) has one of the strongest familial risks all cancers, as evidenced by eight-fold increased risk seen in relatives CLL patients, yet much heritable remains unexplained [1]. Patients with also have a high rate secondary cancer, i.e., development second primary malignancy, compared to general population, which may be explained immune dysregulation due and/or its treatment, but environmental and genetic factors [2]. The cancer patients contributes higher...
The significance of positron emission tomography/computed tomography (PET/CT) in chronic lymphocytic leukemia (CLL) has not yet been systematically studied. This prospective study was aimed at assessing the benefit PET/CT patients with newly diagnosed or relapsed CLL and Richter transformation (RT). examination performed 23 disease, 13 disease eight suspected histopathologically confirmed RT. In all patients, maximum standardized uptake value (SUV(max)) calculated. median SUV(max) 3.4...
Abstract Extramedullary disease (EMM) represents a rare, aggressive and mostly resistant phenotype of multiple myeloma (MM). EMM is frequently associated with high-risk cytogenetics, but their complex genomic architecture largely unexplored. We used whole-genome optical mapping (Saphyr, Bionano Genomics) to analyse the CD138+ cells isolated from bone-marrow aspirates an unselected cohort newly diagnosed patients (n = 4) intramedullary MM 7). Large intrachromosomal rearrangements (> 5 Mbp)...
A growing body of studies highlights involvement neutrophils in cancer development and progression. Our aim was to assess the phenotypic functional properties circulating from patients with chronic lymphocytic leukemia (CLL). The percentage CD54+ CD64+ as well CD54 expression on these cells were higher CLL than age-matched healthy controls. Neutrophils produced more reactive oxygen species (ROS) compared controls both resting activated conditions. Lipopolysaccharide-induced production IL-1β...
Patients with chronic lymphocytic leukemia (CLL) have a higher risk of developing other malignancies (OMs) compared to the general population. However, impact CLL-related factors and CLL-directed treatment is still unclear represents focus this work. We conducted retrospective international multicenter study assess incidence OMs detect potential in 19,705 patients CLL, small lymphoma, or high-count CLL-like monoclonal B-cell lymphocytosis, diagnosed between 2000 2016. Data collection took...
Background Absolute lymphocyte count (ALC) and absolute monocyte (AMC) have been documented as independent predictors of survival in patients with newly diagnosed Diffuse Large B-cell Lymphoma (DLBCL). Analysis the prognostic impact ALC AMC context International Prognostic Index (IPI) other significant variables elderly population treated R-CHOP regime has not carried out yet. Methodology/Principal Findings In this retrospective study, a cohort 443 DLBCL age ≥60 was analyzed. All were...
Positron emission tomography using 2-[fluorine-18]-fluoro-2-deoxy-D-glucose ((18) F-FDG) is considered to be the most beneficial imaging method for staging patients with non-Hodgkin's lymphoma (NHL). The intensity of (18) F-FDG accumulation may determined by calculating so-called standardised uptake value (SUV). study aimed at assessing benefit SUV(max) determination in PET/CT untreated NHL.One hundred and forty-nine initial scans performed NHL between January 2007 August 2009 were assessed,...
Cases of synchronous non-Hodgkin lymphoma (NHL) and second primary carcinoma in previously untreated immunocompetent patients are relatively rare. The aim this part our prospective study was to a revealed 2-F-18 fluoro-2-deoxy-D-glucose (F-18 FDG) positron emission tomography (PET)-positive lesion an extranodal organ suggestive neoplasm newly diagnosed NHL patients.A total 209 with underwent initial staging F-18 FDG PET/computed (CT). finding assessed by radiologist, nuclear medicine...
Antibody (rituximab) dependent cellular cytotoxicity is a key mechanism in killing CD20+ lymphoma cells. FcγRIIIA-158 V/F gene polymorphism results expression of 3 variants the FcγRIIIA receptor (FcγRIIIA) on cytotoxic lymphocytes with different affinity. We studied 102 patients newly diagnosed FL to assess whether genotype influences outcome treated risk-adapted immunochemotherapy. The median age was 52 years (31-84); 90% had advanced (III/IV) clinical stages. Follicular Lymphoma...
Background The data on the clinical utility of quantitative assessment immunophenotypes in distinguishing mature CD5‐positive B‐cell neoplasms is limited. study aim was to assess diagnostic value a panel 18 markers and identify most informative ones. Methods immunophenotype neoplastic population determined specimens from 188 patients. BD FACSCanto II flow cytometer FACSDiva software were used analyze positivity/negativity mean fluorescence intensity (MFI) surface expression markers. Advanced...
Serum protein fingerprints associated with MGUS and MM their changes in after autologous stem cell transplantation (MM-ASCT, day 100) remain unexplored. Using highly-sensitive Proximity Extension ImmunoAssay on 92 cancer biomarkers (Proseek Multiplex, Olink), enhanced serum levels of Adrenomedullin (ADM,
Array-based comparative genomic hybridization (arrayCGH) studies in chronic lymphocytic leukemia (CLL) have revealed novel recurrent chromosomal imbalances, such as a gain of chromosome 2p. However, detailed cytogenetic analysis the 2p region has not been elucidated. Here, we present and molecular 16 cases selected from group 200 patients with CLL based on CGH and/or arrayCGH data. We significant heterogeneity CLL, including new aberration: dicentric chromosome, dic(2;18). In our cases,...
The programmed cell death pathway is involved in functional impairment of cytotoxic CD8+ T cells chronic viral infection and tumor immune evasion. interaction death-1 (PD-1) with its ligand suppresses antitumor function stimulates the regulatory population. objectives were to investigate whether examining PD-1 expression peripheral patients lymphocytic leukemia (CLL) reflected disease phase Binet stage compare results those healthy volunteers. study analyzed blood from previously untreated...
Peripheral T-cell lymphomas (PTCL) are infrequent subtypes of non-Hodgkin's lymphomas. The clinical course is aggressive and the median survival about 2-3 years. An optimal first-line chemotherapy protocol has not been established role high-dose therapy with autologous stem cell transplantation (ASCT) still unclear.To analyze long-term outcome unselected PTCL patients treated intensive ASCT.Here we report our experience 29 PTCL. histological were as follows: peripheral lymphoma, otherwise...
MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules that regulate gene expression at the post-transcriptional level. In pathogenesis of chronic lymphocytic leukemia (CLL), miR-15a and miR-16-1 play an important role. These miRNAs located on chromosome 13 in 13q14.3 region, which is deleted more than 55% CLL patients. This aberration affects miRNAs.The study aimed performing a molecular genetic analysis group 39 patients diagnosed with determining association between two...
Serum thymidine kinase 1 (TK1) is a sensitive marker of tumor cell proliferation. TK1 has been reported as reliable prognostic factor in solid tumors and chronic lymphocytic leukemia, but not yet tested large populations patients with non-Hodgkin lymphoma. In this study, the significance levels was assessed 170 prospectively enrolled previously untreated follicular lymphoma (FL). The level at time treatment initiation shown to correlate clinical stage, Follicular Lymphoma International...