A5100644869- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Geothermal Energy Systems and Applications
- Cancer Genomics and Diagnostics
- Histone Deacetylase Inhibitors Research
- Circular RNAs in diseases
- TGF-β signaling in diseases
- Glycosylation and Glycoproteins Research
- DNA and Nucleic Acid Chemistry
- Marine Biology and Environmental Chemistry
- Congenital heart defects research
- Advanced Glycation End Products research
- Retinopathy of Prematurity Studies
- Cardiac Arrest and Resuscitation
- Gynecological conditions and treatments
- DNA Repair Mechanisms
- Diet, Metabolism, and Disease
- Protease and Inhibitor Mechanisms
Fudan University
2019
Taian City Central Hospital
2013-2019
Ningbo Institute of Industrial Technology
2018
Chinese Academy of Sciences
2018
ShanghaiTech University
2018
Tongji University
2011-2016
Shanghai Pulmonary Hospital
2016
Nanjing Medical University
2016
Jiangsu Province Hospital
2016
Jinzhou Medical University
2015
Abstract Background/Aims Hepatocellular carcinoma ( HCC ), cholangiocarcinoma CC ) and hepatoblastoma HB are the main hepatic malignancies with limited treatment options high mortality. Recent studies have implicated H ippo kinase pathway in cancer development, but detailed analysis of signalling human malignancies, especially , is lacking. Methods We investigated using cells patient samples. Results Increased expression yes‐associated protein Y ap), downstream effector pathway, was observed...
microRNAs are a small class of non-coding RNAs with critical role in the tumorigenesis and maintenance breast cancer through binding to 3'-untranslated regions target mRNAs, which causes block translation and/or mRNA degradation. The purpose this study was investigate expression microRNA-497 (miR-497) as well its potential human cancer. Reverse transcription-polymerase chain reaction (RT-PCR) performed determine pattern miR-497 normal tissues. Correlation analysis conducted characterize...
OTX1 Contributes to Hepatocellular Carcinoma Progression by Regulation of ERK/MAPK PathwayOrthodenticlehomeobox 1 (OTX1) overexpression had previously been associated with the progression several tumors.The present study aimed determine expression and role in human hepatocellular carcinoma (HCC).The level was examined quantitative real-time PCR (qRT-PCR) 10 samples HCC paired adjacent non-cancerous tissues, immunohistochemistry (IHC) analysis 128 matched controls.The relationship between...
<b><i>Purpose:</i></b> To explore the expression of Yes-associated protein (YAP) in hepatocellular carcinoma (HCC) patients, and assess its prognostic value to recurrence HCC after liver transplantation (LT). <b><i>Methods:</i></b> Collected data 105 consecutive patients undergoing LT for were analyzed retrospectively. The immunohistochemistry was used detect YAP, Mst1, Lats1/2, pYAP, pLats1/2 pMst1/2 tumor tissues. Contingency table...
Nε‐(carboxymethyl) lysine (CML), the major member of advanced glycation end products, was widely studied in diabetic complications and aging‐associated diseases. However, impact CML on myocardial ischemia/reperfusion injury (MI/RI) rarely reported. In present study, increased both patients with acute infarction (53.4 ± 7.8 vs. 28.1 4.4 ng; P = 0.017), mice underwent MI/RI (16.4 61.4 10.8 0.9 0.006). Depletion neutrophils reduced (17.8 1.0 9.9 0.3 < 0.001), indicating were cells...
The siRNA deliveries, for the siRNA's high negative charge, short serum half life, poor cellular internalization, etc, are still key barriers its application in clinic. In this study, several cell penetrating peptide (CPP) and dsRNA binding domain (dsRBD)-based fusion proteins have been developed screened as vector. ability was measured by agarose gel retardation, uptaking characterized under fluorescence microscopy, further more RNAi effect evaluated on endogenous (GAPDH, western blot)...
Multiple computational tools have been widely applied to the detection of coding driver mutations in cancer; however, prioritization pathogenic non-coding variants remains a difficult and demanding task. The present study was performed distinguish non‑coding disease‑causing from neutral ones, prioritize potential cancer-associated long RNAs (lncRNAs) with logistic regression model lung cancer. A constructed, combining 19,153 disease‑associated ClinVar Human Gene Mutation Database as response...
Background/Aims Despite great progress in the treatment of hepatocellular carcinoma (HCC) over last-decade, intrahepatic recurrence is still most frequent serious adverse event after all treatments including microwave ablation. This study aimed to predict early HCC ablation using serum proteomic signature. Methods After curative HCC, 86 patients were followed-up for 1 year. Serum samples collected before The mass spectra proteins generated surface-enhanced laser desorption/ionization...
BACKGROUND:Intrauterine adhesions (IUAs) are one of the most common reproductive system diseases in women worldwide. Emerging evidence has demonstrated that upregulation or downregulation genes plays an important role IUAs. The aim this study was to evaluate NUS1 IUAs a rat model. MATERIAL AND METHODS:RT-qPCR, IHC, and Western blot were used investigate mRNA proteins expression, respectively, NUS1. MTT colony-formation assays cell growth. Transwell detect migration invasion. To miR-466...
There are multiple bioinformatics tools available for the detection of coding driver mutations in cancers. However, prioritization pathogenic non-coding variants remains a challenging and demanding task. The present study was performed to discriminate disease-causing prioritize potential cancer-implicated long RNAs (lncRNAs) liver cancer using logistic regression model. A model constructed by combining 19,153 disease-associated ClinVar human gene mutation database as response variable...
Inhibitor of growth protein 2 (ING2) has an important role in the regulation chromatin remodeling, cell proliferation, cell‑cycle arrest, senescence and apoptosis. The present study performed immunohistochemical analysis for expression profiling ING2 array tissues comprising normal mouse human tissues, as well hepatocellular (n=62), renal clear pancreatic esophageal squamous (n=45), cervical (n=31), breast (n=144), gastric (n=196), colorectal (n=96), ovarian (n=208), endometrial (n=96) lung...
To explore the effects of LYRM1 knockdown on proliferation, apoptosis, differentiation and mitochondrial function in embryonic carcinoma (P19) cell model cardiac differentiation. Knockdown using small interfering RNA (siRNA) was confirmed by quantitative real-time PCR. Cell Counting Kit-8(CCK-8) proliferation assays cycle analysis demonstrated that gene silencing significantly inhibited P19 proliferation. Flow cytometry measurement their caspase-3 activities revealed increased apoptosis....
Pancreatic-duodenal homeobox 1 (PDX-1) is a transcription factor that regulates embryological pancreas development and insulin expression in adult islets. The current study investigated the profile potential role of PDX-1 breast cancer. Immunohistochemistry was performed to determine pattern cancer adjacent benign tissues. In addition, cell proliferation cycle were evaluated following transient inhibition with antisense oligonucleotides MCF-7 human cells. Real-time PCR western blotting...
The aim of this study was to investigate the expression Runt box 3 (RUNX3) and aplasia Ras homolog member I (ARHI) in ovarian tumors, effects histone deacetylase inhibitors (HDACIs) on these genes growth cancer cells. mRNA RUNX3 ARHI normal ovaries tumors determined using reverse transcription polymerase chain reaction (RT-PCR). HDACIs four cell lines (SKOV3, A2780, COC1 OC3) were RT-PCR MTT assay. cells 86 100%, respectively. Although two downregulated extent downregulation differed....
The groundwater source heat pump (GWHP) system utilizes as alternative renewable and clean energy for space heating cooling, withdraws water from a production well, passes it through exchanger discharges the into an injection well. Hebei Province of China is rich geothermal resources; there are suitable hydrogeological conditions in most areas. Take office building example; detail loads analysis was conducted. At same time, environmental benefits also Comparing with traditional systems...
Objective To explore the effect of Rab26 on Proliferation and migration human in small cell lung cancer cells(H446). Methods The H446 cells were transfected with over-expression vector Rab26, or siRNA. Blank group was used as a negative control, respectively. After transfection 48 h, protein level mRNA determined by RT-PCR Western blot. assayed flow cytometry rate detected scratch test. Results The efficiency overexpression plasmid (76.8±4.3) % siRNA (79.5±3.57)%. hours,...