A5000348773- Cancer Mechanisms and Therapy
- Endometrial and Cervical Cancer Treatments
- Estrogen and related hormone effects
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- HER2/EGFR in Cancer Research
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Ovarian cancer diagnosis and treatment
- Signaling Pathways in Disease
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- 14-3-3 protein interactions
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- Aquaculture disease management and microbiota
- Biochemical and Structural Characterization
- Protein Degradation and Inhibitors
- Vector-borne infectious diseases
Hunan Agricultural University
2023-2025
Hybrid Rapeseed Research Center of Shaanxi Province
2025
Nantong University
2025
Fujian Agriculture and Forestry University
2021-2025
University of Iowa
2015-2024
Ministry of Education of the People's Republic of China
2024
Northwest University
2024
Chengdu University
2023
Hebei Normal University
2012-2014
University of Iowa Hospitals and Clinics
2013-2014
Mammalian cells respond to endoplasmic reticulum (ER) stress by attenuation of protein translation mediated through the PERK-eIF2alpha pathway and transcriptional activation genes such as Grp78/BiP encoding ER chaperone proteins. The disruption PERK function or blocking eIF2alpha Ser51 phosphorylation fails attenuate after also results in substantial impairment induction stress. While Grp78 promoter ATF6 elements (ERSEs) is well documented, molecular mechanism linking unknown. We report here...
Vascular endothelial growth factor (VEGF) plays a key role in the development and progression of diabetic retinopathy. We previously demonstrated that amino acid deprivation other inducers endoplasmic reticulum-stress (ER stress) up-regulate expression VEGF retinal-pigmented epithelial cell line ARPE-19. Because homocysteine causes ER stress, we hypothesized is increased by ambient homocysteine. dl-Homocysteine-induced was investigated confluent ARPE-19 cultures. Northern analysis showed...
Seed color is a critical quality trait in numerous plant species. In oilseed Brassica crops, including rapeseed and mustard, yellow seeds are distinguished by their significantly higher oil content faster germination rates compared to black or brown counterparts. Despite the agronomic significance of being prime breeding target, mechanisms underlying elevated remain obscure. this study, we assembled first telomere-to-telomere (T2T) genome B. juncea further investigated genetic regulation,...
Often considered to be a "dead" kinase, erbB3 is implicated in escape from erbB-targeted cancer therapies. Here, heregulin stimulation shown markedly upregulate kinase activity immunoprecipitates. Intact, activated phosphorylates tyrosine sites an exogenous peptide substrate, and this abolished by mutagenesis of lysine 723 the catalytic domain. Enhanced linked heterointeractions with catalytically active erbB2, since it largely blocked cells pretreated lapatinib or pertuzumab. erbB2...
Ferroptosis is an iron-dependent, non-apoptotic form of regulated cell death driven by lipid hydroperoxides within biological membranes. Although therapy-resistant mesenchymal-high cancers are particularly vulnerable to ferroptosis inducers, especially phospholipid glutathione peroxidase 4 (GPx4) inhibitors, the underlying mechanism yet be deciphered. As such, full application GPx4 inhibitors in cancer therapy remains challenging. Here we demonstrate that metadherin (MTDH) confers a state...
Distributions of ErbB receptors on membranes SKBR3 breast cancer cells were mapped by immunoelectron microscopy. The most abundant receptor, ErbB2, is phosphorylated, clustered and active. Kinase inhibitors ablate ErbB2 phosphorylation without dispersing clusters. Modest co-clustering EGFR, even after EGF treatment, suggests that both are predominantly involved in homointeractions. Heregulin leads to dramatic clusters ErbB3 contain some EGFR PI 3-kinase. Other docking proteins, such as Shc...
Tumor suppressor p53 is responsible for enforcing cell cycle checkpoints at G1/S and G2/M in response to DNA damage, thereby allowing both normal tumor cells repair before entering S M. However, with absent or mutated are able activate alternative signaling pathways that maintain the checkpoint, which becomes uniquely critical survival of such cells. We hypothesized abrogation G2 checkpoint might preferentially sensitize p53-defective DNA-damaging agents spare intact function. The tyrosine...
This study proposes a compound low-sodium alternative salt (CLSAS) formulation (2.4% sodium chloride, 0.8% K lactate, 0.4% magnesium Ca ascorbate, 0.2% L lysine, and 4% sorbitol) combined with vacuum tumbling for beef marination. The effects of NaCl static marination (F), CLSAS (L), (VT-L) on the physicochemical properties, water distribution, microstructure marinated were evaluated. Compared F, maintained similar yield color, reduced cooking loss, improved texture while lowering content....
Abstract Background A stochastic simulator was implemented to study EGFR signal initiation in 3D with single molecule detail. The model considers previously unexplored contributions receptor-adaptor coupling, such as receptor clustering and diffusive properties of both receptors binding partners. agent-based rule-based approach permits consideration combinatorial complexity, a problem associated multiple phosphorylation sites the potential for simultaneous adaptors. Results used simulate...
Dysregulation of the mammalian target rapamycin (mTOR) signaling has been found in many human cancers, particularly those with loss tumor suppressor PTEN. However, mTORC1 inhibitors such as temsirolimus have only modest activity when used alone and may induce acquired resistance by activating upstream mTORC2 Akt. Other tumors that do not depend upon PI3K/Akt/mTOR for survival are primarily resistant. This study tested hypothesis limited clinical efficacy is due to a compensatory increase...
Understanding the molecular underpinnings of chemoresistance is vital to design therapies restore chemosensitivity. In particular, metadherin (MTDH) has been demonstrated have a critical role in chemoresistance. Over-expression MTDH correlates with poor clinical outcome breast cancer, neuroblastoma, hepatocellular carcinoma and prostate cancer. also highly expressed advanced endometrial cancers, disease for which new are urgently needed. this present study, we focused on therapeutic benefit...
Chemoresistance and metastasis are the main reasons for failure of current treatments with sarcoma patients. Novel biomarkers required to predict response treatment. The oncogene MTDH/AEG1 long noncoding RNA (lincRNA) HOTAIR two novel factors involved in drug resistance various types solid tumors. However, correlation between MTDH/AEG-1 expression is unknown.Expression MTDH protein or was detected by Western blotting qRT-PCR, respectively, primary metastatic patient tissue samples.High...
// Shujie Yang 1, 4 , Yichen Jia 1 Xiaoyue Liu Christopher Winters Xinjun Wang Yuping Zhang Eric J. Devor Adriann M. Hovey Henry D. Reyes Xue Xiao Xu 2 Donghai Dai Xiangbing Meng Kristina W. Thiel Frederick E. Domann 3, Kimberly K. Leslie Department of Obstetrics and Gynecology, University Iowa, IA, 52242, USA The Interdisciplinary Graduate Program in Informatics, 3 Free Radical Radiation Biology Program, Oncology, Carver College Medicine Holden Comprehensive Cancer Center, Correspondence...
Endometrial cancer, the most common gynecologic malignancy, is a hormonally-regulated tumor. Response to progestin-based therapy correlates positively with progesterone receptor (PR) expression. However, many endometrial tumors have low levels or loss of PR, limiting clinical application progestin. We evaluated ability epigenetic modulators restore functional PR expression in Type I cancer cells basal PR. Treatment histone deacetylase inhibitor (HDACi) LBH589 induced profound upregulation...
Endometrial cancer, the most common gynecologic malignancy, is a hormonally-regulated disease. Response to progestin therapy positively correlates with hormone receptor expression, in particular progesterone (PR). However, many advanced tumors lose PR expression. We recently reported that efficacy of can be significantly enhanced by combining epigenetic modulators, which we term "molecularly therapy." What remained unclear was mechanism action and if estrogen α (ERα), principle inducer PR,...