A5006092011- Systemic Lupus Erythematosus Research
- Renal Diseases and Glomerulopathies
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- Complement system in diseases
- Immunotherapy and Immune Responses
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Vasculitis and related conditions
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- interferon and immune responses
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Electromagnetic Fields and Biological Effects
- Immune Cell Function and Interaction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell death mechanisms and regulation
- Chronic Lymphocytic Leukemia Research
- Autophagy in Disease and Therapy
- Sulfur Compounds in Biology
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- Ocular Diseases and Behçet’s Syndrome
Centre National de la Recherche Scientifique
2007-2025
Centre Hospitalier Universitaire de Grenoble
2016-2025
Institut de Biologie Structurale
2023-2025
CEA Grenoble
2023-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2023-2025
Université Grenoble Alpes
2016-2025
Inserm
2005-2025
Institut pour l'avancée des biosciences
2025
Translational Innovation in Medicine and Complexity
2016-2022
Institut polytechnique de Grenoble
2022
Whether protein translation occurs in the nucleus is contentious. To address this question, we developed ribopuromycylation method (RPM), which visualizes cells via standard immunofluorescence microscopy. The RPM based on ribosome-catalyzed puromycylation of nascent chains immobilized ribosomes by antibiotic chain elongation inhibitors followed detection puromycylated ribosome-bound with a puromycin (PMY)-specific monoclonal antibody fixed and permeabilized cells. correlates localized myriad...
Nucleic acid sensing by cells is a key feature of antiviral responses, which generally result in type-I Interferon production and tissue protection. However, detection double-stranded RNAs virus-infected promotes two concomitant apparently conflicting events. The dsRNA-dependent protein kinase (PKR) phosphorylates translation initiation factor 2-alpha (eIF2α) inhibits synthesis, whereas cytosolic DExD/H box RNA helicases induce expression type I-IFN other cytokines. We demonstrate that the...
Blast quantification in the bone marrow (BM) is crucial for evaluating myeloid neoplasms, with cytomorphology being only recognized analysis. The CD34 cell (CD34M) count by flow cytometry promising but impaired BM hemodilution. A modified version of Holdrinet index (mHI) routinely used to assess it, though not yet validated. By analyzing two differently hemodiluted samples from 51 patients suspicion myelodysplasia, this study confirms mHI accuracy assessing white blood purity. mHI-adjusted...
In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation that exhibits specific mechanisms control the immune response. Here we show in polyriboinosinic:polyribocytidylic acid (pI:C), DCs mount integrated stress during which transcription factor ATF4 and growth arrest DNA damage-inducible protein 34 (GADD34/Ppp1r15a), phosphatase 1 (PP1) cofactor, are expressed. agreement with increased GADD34 levels, an extensive dephosphorylation...
A significant portion of newly synthesized protein fails to fold properly and is quickly degraded. These defective ribosomal products (DRiPs) are substrates for the ubiquitin-proteasome system (UPS) give rise a large fraction peptides presented by major histocompatibility complex class I molecules (MHCI). Here, we showed that DRiPs also autophagy substrates, which accumulate upon inhibition in aggresome-like-induced structures (ALIS). Aggregation critically depending on p62/SQSTM1, but...
A precise identification and phenotypic characterization of human B-cell subsets is crucial importance in both basic research medicine. In the literature, flow cytometry studies for B-lymphocytes are mainly focused on description a particular cell stage, or specific stages observed single type sample. present work, we propose backbone 6 antibodies (CD38, CD27, CD10, CD19, CD5 CD45) an efficient gating strategy to identify, analysis tube, large number covering whole differentiation from...
The SARS-CoV-2 infection triggers excessive immune response resulting in increased levels of pro-inflammatory cytokines, endothelial injury, and intravascular coagulopathy. complement system (CS) activation participates to this hyperinflammatory response. However, it is still unclear which pathways (classical, alternative, or lectin pathway) pilots the effector mechanisms that contribute critical illness. To better understand correlates disease severity, we performed an analysis CS...
In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms control antigen processing and presentation. Here, we show in lipopolysaccharides, protein synthesis is rapidly enhanced DCs. This enhancement occurs via PI3K-dependent signaling pathway key for DC activation. addition, later on, manner similar viral or apoptotic stress, activation leads the phosphorylation proteolysis important...
Anti-glomerular basement-membrane (anti-GBM) disease (or Goodpasture disease) is characterized by severe kidney and lung involvement. Prognoses have improved with treatments that combine plasma exchange immunosuppressive drugs. However, patients renal involvement can poor outcomes cyclophosphamide cause significant complications. Anti-GBM antibodies a direct pathogenic effect on the disease: thus, therapeutics decrease their production, such as rituximab, could be good alternative.The...
Dendritic cells (DCs) are the sentinels of mammalian immune system, characterized by a complex maturation process driven pathogen detection. Although multiple studies have described analysis activated DCs transcriptional profiling, recent findings indicate that mRNAs also regulated at translational level. A systematic being translationally various stages DC activation was performed using which combines sucrose gradient fractionation polysomal-bound with DNA microarray analysis.Total and mRNA...
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of multiple autoantibodies. Antibodies against Ficolin-3 were previously identified in sera some SLE patients, but their prevalence and significance have not been yet investigated. The aims this study to determine anti-ficolin-3 antibodies among patients investigate potential as diagnostic and/or prognostic biomarkers SLE. In retrospective study, from (n = 165) selected preexisting declared...
Glutathione depletion by inhibition of its synthesis with buthionine sulfoximine (BSO) is a focus the current research in antitumor therapy, BSO being used as chemosensitizer. We had previously shown that two human tumor cell lines (U937 and HepG2) survive to treatment BSO: can elicit an apoptotic response, but process aborted after cytochrome c release before caspase activation, suggesting development adaptive response (FASEB J., 1999, 13, 2031-2036). Here, we investigate mechanisms such...
The essential roles of the endovacuolar system in health and disease call for development new tools allowing a better understanding complex molecular machinery involved endocytic processes. We took advantage floating properties small latex beads (sLB) on discontinuous sucrose gradient to isolate highly purified endosomes following internalization J774 macrophages bone marrow-derived dendritic cells (DC). particularly focused isolation early late endosomes/lysosomes (LE/LYS) as well LE/LYS...
Systemic lupus erythematosus (SLE) is a multisystem inflammatory disease characterized by the production of various autoantibodies. The aim this study was to investigate presence anti-ficolin-2 antibodies in SLE patients and evaluate association between levels these autoantibodies, clinical manifestations, activity.This comparative using cohort 165 48 healthy subjects. were further divided into 2 groups (low activity [SLE Disease Activity Index (SLEDAI) score ≤4, n = 88] high [SLEDAI >4,...
Growth arrest and DNA damage-inducible gene 34 (GADD34) is an inducible cofactor of protein phosphatase 1, which has important role in the unfolded response. GADD34 been shown to be necessary for type I interferon proinflammatory cytokine production response viral infection murine models. We investigate expression rheumatoid arthritis (RA), cytokines have pathogenic role. The objective this study was evaluate potential as a biomarker RA patients. report case-control on peripheral blood...
COVID-19 is caused by the human pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has resulted in widespread morbidity mortality. CD4 + T cells, CD8 cells neutralizing antibodies all contribute to control SARS-CoV-2 infection. However, heterogeneity a major factor disease severity immune innate adaptive responses SARS-CoV-2. We performed deep analysis flow cytometry of lymphocyte populations 125 hospitalized infected patients on day hospital admission. Five clusters...
The tumor-suppressor protein p53 is an intracellular critical in many biochemical cascades leading to cell death via apoptosis pathway or survival by arresting the cycle. Based on recent studies, which have characterized chemical inhibitors of cancer therapy field as well pathogenesis stroke and neurodegenerative disorders, this review concentrates development drugs that inhibit their possible use novel strategies for combating diseases, cancer, eventually HIV infection. Emphasis mainly...