A5102747556- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Diet and metabolism studies
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Metabolomics and Mass Spectrometry Studies
- Cancer Mechanisms and Therapy
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Bioinformatics and Genomic Networks
- Helicobacter pylori-related gastroenterology studies
- Machine Learning in Bioinformatics
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Oral microbiology and periodontitis research
- Genetics, Bioinformatics, and Biomedical Research
- Mitochondrial Function and Pathology
- Discourse Analysis in Language Studies
Beijing Proteome Research Center
2018-2025
Affiliated Hospital of Qingdao University
2014
Qingdao University
2014
Shandong Provincial QianFoShan Hospital
2011
China People's Police University
2010
Despite medical advances, there remains an unmet need for better treatment of obesity. Itaconate, a product the decarboxylation tricarboxylic acid cycle intermediate cis-aconitate, plays regulatory role in both metabolism and immunity. Here, we show that itaconate, as endogenous compound, counteracts high-fat-diet (HFD)-induced obesity through leptin-independent mechanisms three mouse models. Specifically, itaconate reduces weight gain, reverses hyperlipidemia, improves glucose tolerance...
Quantitative proteomics is an indispensable tool in life science research. However, there a lack of reference materials for evaluating the reproducibility label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements among different instruments and laboratories.Here, we develop Quartet standard as proteome material with built-in truths, distribute same aliquots to 15 laboratories nine conventional LC-MS/MS platforms across six cities China. Relative abundance over...
The mammalian stomach is structurally highly diverse and its organ functionality critically depends on a normal embryonic development. Although there have been several studies the morphological changes during development, system-wide analysis of underlying molecular lacking. Here, we present comprehensive, temporal proteome transcriptome atlas mouse at multiple developmental stages. Quantitative 12,108 gene products allows identifying three distinct phases based in proteins RNAs gain...
Abstract The human gastric mucosa is the most active layer of stomach wall, involved in food digestion, metabolic processes and carcinogenesis. Anatomically, divided into seven regions, but protein basis for cellular specialization not well understood. Here we present a global analysis profiles 82 apparently normal samples obtained from living individuals by endoscopic biopsy. We identify 6,258 high-confidence proteins estimate ranges expression presenting region-resolved proteome reference...
Liver organogenesis and development are composed of a series complex, well-orchestrated events. Identifying key factors pathways governing liver will help elucidate the physiological pathological processes including those cancer. We conducted multidimensional omics measurements protein, mRNA, transcription factor (TF) DNA-binding activity for mouse tissues collected from embryonic day 12.5 (E12.5) to postnatal week 8 (W8), encompassing major developmental stages. These data sets reveal...
DNA modifications, represented by 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), play important roles in epigenetic regulation of biological processes. The specific recognition modifications the transcriptional protein machinery is thought to be a potential mechanism for epigenetic-driven gene regulation, many modified DNA-specific binding proteins have been uncovered. However, panoramic view modification readers at proteome...
In orthotopic mouse tumor models, progression is a complex process, involving interactions among cells, host cell-derived stromal and immune cells. Much attention has been focused on the its microenvironment, while host's macroenvironment including organs in response to tumorigenesis poorly understood. Here, we report temporal proteomic analysis subcutaneous three (LN, MLN, spleen) collected Days 0, 3, 7, 10, 14, 21 after inoculation of forestomach cancer cells syngeneic model....
Abstract Quantitative proteomics is an indispensable tool in life science research. However, there a lack of reference materials for evaluating the reproducibility label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements among different instruments and laboratories. We developed Quartet as proteome material with built-in truths, distributed same aliquots to 15 laboratories nine conventional LC-MS/MS platforms across six cities China. Relative abundance over...
Human leucocyte antigen class I (HLA-I), which includes HLA-A, -B and -C, is an essential immune factor participating in the antitumour response. The changes HLA-I expression peripheral blood T lymphocytes cancer patients have yet to be defined. This study examined of on CD4 + CD8 female with stage — IV breast infiltrating ductal carcinoma, benign tumour diseases (mammary intraductal papilloma or fibroadenoma), healthy controls. was down-regulated from III cancer, compared may contribute...
<title>Abstract</title> <bold>Background</bold> The aim of this study was to establish a primary mouse gastric carcinoma cell line. <bold>Methods</bold> Gastric adenocarcinoma in the body region induced immunocompetent BALB/c mice using N-Methyl-N-nitrosourea and 2% NaCl solution. Fresh cancer tissue samples were cultured 1640 medium supplemented with 10% fetal bovine serum for culture subculture. Cellular morphology assessed via light microscopy, growth curve established. Genomic proteomic...
To explore the effect of high fat diet on proteome in mice stomachs, we constructed a model which were fed with as (HFD) group or normal control (CTRL) for 110 days. The stomachs collected and divided into three regions (forestomach (F), corpus (C) antrum (A)) protein extraction mass spectrometry analysis. Of all 9 307 identified proteins two groups, 4 066 (HFD: 3 832, CTRL: 654) strictly by at least one unique peptide twice replicates. Using gene ontology (GO) interaction network analysis...
Despite advances in medicine, there is still an unmet need for better treatment of obesity. Itaconate as a product the decarboxylation TCA cycle intermediate cis-aconitate, regulates numerous biological processes. Here we show that itaconate endogenous compound counteracts high fat diet (HFD)-induced obesity through leptin-independent mechanisms three mice models. Specifically, inhibits weight gain, reverses hyperlipidemia, and improves insulin sensitivity HFD-fed mice. Additionally, able to...