A5036430313- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Advanced Proteomics Techniques and Applications
- Cancer Research and Treatments
- Telomeres, Telomerase, and Senescence
- Occupational and environmental lung diseases
- Cancer, Lipids, and Metabolism
- Vanadium and Halogenation Chemistry
- Venomous Animal Envenomation and Studies
- Chemical Synthesis and Reactions
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Protein Hydrolysis and Bioactive Peptides
- Ubiquitin and proteasome pathways
- Breast Cancer Treatment Studies
- Phytochemical compounds biological activities
- Glioma Diagnosis and Treatment
- Ion channel regulation and function
- Traditional and Medicinal Uses of Annonaceae
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
Inserm
2015-2025
Université d'Angers
2015-2025
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2012-2025
Centre National de la Recherche Scientifique
2012-2025
Nantes Université
2017-2025
Institut de Cancérologie de l'Ouest
2011-2025
Institut Génétique Nantes Atlantique
2023
Centre de recherche Translationnelle en Médecine moléculaire
2015
Gefluc Languedoc Roussillon
2014
Paul Pascal Research Center
2009-2013
Triple-negative breast cancers need to be refined in order identify therapeutic subgroups of patients.We conducted an unsupervised analysis microarray gene-expression profiles 107 triple-negative cancer patients and undertook robust functional annotation the molecular entities found by means numerous approaches including immunohistochemistry signatures. A external cohort (n=87) was used for validation.Fuzzy clustering separated tumours into three clusters: C1 (22.4%), C2 (44.9%) C3 (32.7%)....
Arthropod borne virus infections cause several emerging and resurgent infectious diseases. Among the diseases caused by arboviruses, dengue chikungunya are responsible for a high rate of severe human worldwide. The midgut mosquitoes is first barrier pathogen transmission target organ where arboviruses must replicate prior to infecting other organs. A proteomic approach was undertaken characterize key virus/vector interactions host protein modifications that happen in viral eventually take...
Expression profiles represent new molecular tools that are useful to characterize the successive steps of tumor progression and prediction recurrence or chemotherapy response. In this study, we have used quantitative proteomic analysis compare different stages colorectal cancer. A combination laser microdissection, OFFGEL separation, iTRAQ labeling, MALDI-TOF/TOF MS was explore proteome 28 cancer tissues. Two software packages were for identification quantification differentially expressed...
Quercitrin (quercetin 3- O-alpha- L-rhamnopyranoside), one of the constituents biologically active aqueous extract obtained from Kalanchoe pinnata, is demonstrated to be a potent antileishmanial compound (IC50 approximately 1 microg/mL) with low toxicity profile. This first time that activity for flavonoid glycoside.
Glioblastoma (GB) displays diffusely infiltrative growth patterns. Dispersive cells escape surgical resection and contribute to tumour recurrence within a few centimeters of the cavity in 90% cases. We know that non-neoplastic stromal compartment, addition cells, plays an active role recurrence. isolated new cell population from histologically normal margins GB by computer-guided stereotaxic biopsies primary culture. These GB-associated (GASCs) share phenotypic functional properties with...
Abstract Senescence is a tumor-suppressive mechanism induced by telomere shortening, oncogenes, or chemotherapy treatment. Although it clear that this suppressive pathway leads to permanent arrest in primary cells, might not be the case cancer cells have inactivated their pathways. We recently shown subpopulations of can escape chemotherapy-mediated senescence and emerge as more transformed induce tumor formation, resist anoikis, are invasive. In study, we characterized emergence showed...
Abstract Senescence is a tumor suppressive mechanism that induces permanent proliferative arrest in response to an oncogenic insult or the genotoxic stress induced by chemotherapy. We have recently described some cells can escape this arrest, either because senescence was incomplete as consequence of phenotypic adaptation. Malignant which resisted emerged more transformed resist anoikis and rely on survival pathways activated Akt Mcl-1. In study, we further characterize escape, investigating...
Human blood plasma contains a plethora of proteins, encompassing not only proteins that have plasma-based functionalities, but also possibly every other form low concentrated human proteins. As it circulates through the tissues, picks up are released from their origin due to physiological events such as tissue remodeling and cell death. Specific disease processes or tumors often characterized by “signatures,” which may become obvious via changes in proteome profile, for example, over...
The development of mass spectrometric techniques and fractionation methods now allows the investigation very complex protein mixtures ranging from subcellular structures to tissues. Nevertheless, this work is particularly difficult due wide dynamic range concentration in eukaryotic In paper, we present a shotgun method whereby peptides are fractionated using OFFGEL electrophoresis after iTRAQ labelling.We demonstrated that peptide labelling enhances MALDI ionisation labelled introduces...
To date, there is no available targeted therapy for patients who are diagnosed with triple-negative breast cancers (TNBC). The aim of this study was to identify a new specific target treatments. Frozen primary tumors were collected from 83 adjuvant therapy-naive TNBC patients. These samples used global proteome profiling by iTRAQ-OFFGEL-LC-MS/MS approach in two series: training cohort (n = 42) and test set 41). Patients remains free local or distant metastasis minimum 5 years after surgery...
Senescence is a tumor suppressor mechanism triggered by oncogene expression and chemotherapy treatment. It orchestrates definitive cessation of cell proliferation through the activation p53-p21 p16-Rb pathways, coupled with compaction proliferative genes within heterochromatin regions. Some cancer cells have ability to elude this arrest but signaling pathways involved in circumventing senescence remain be characterized. We recently described that malignant capable evading an increased...
The tumor microenvironment (TME) of non-small cell lung cancer (NSCLC) is highly heterogeneous and involved in tumorigenesis resistance to therapy. Among the cells TME, endothelial are associated with latter processes through endothelial-to-mesenchymal transition (EndMT). During EndMT, (ECs) progressively lose their phenotype favor a mesenchymal phenotype, which favors production cancer-associated fibroblasts (CAFs). Our study aimed investigate consequences exposure different secretomes on...
// Barbara Jonchère 1 , Alexandra Vétillard Bertrand Toutain David Lam Anne Charlotte Bernard Cécile Henry Sophie De Carné Trécesson 2 Erick Gamelin Philippe Juin 2,3 Catherine Guette and Olivier Coqueret Paul Papin ICO Cancer Center, INSERM U892, CNRS 6299, Angers University, Angers, France Nantes Nantes, 3 Gauducheau Correspondence: Coqueret, email: Keywords : Chemotherapy, senescence, irinotecan, drug resistance, colorectal cancer Received September 10,...
Signal transducers and activators of transcription 3 (STAT3) proteins are cytoplasmic factors that translocate into the nucleus to induce following growth factor or cytokine stimulation. Besides their normal functions, these play an important role in cancer cells through abnormal activation cell cycle progression deregulation survival senescence pathways. New data obtained from laboratory Guido Kroemer identifies STAT3 as a new autophagy regulator. In cytoplasm, absence conventional...
Abstract In tumours, accumulation of chemoresistant cells that express high levels anti-apoptotic proteins such as BCL-X L is thought to result from the counter selection sensitive, low expresser clones during progression and/or initial treatment. We herein show expression selectively advantageous cancer cell populations even in absence pro-apoptotic pressure. transformed human mammary epithelial favours full activation signalling downstream constitutively active RAS with which it interacts...
Two novel peptides that inhibit the intra‐erythrocyte stage of Plasmodium falciparum in vitro were identified venom Trinidad chevron tarantula, Psalmopoeus cambridgei . Psalmopeotoxin I (PcFK1) is a 33‐residue peptide and II (PcFK2) has 28‐amino acid residues; both have three disulfide bridges belong to Inhibitor Cystine Knot superfamily. The cDNAs encoding cloned, nucleotide sequence analysis showed are synthesized with typical signal pro‐sequences cleaved at basic doublet before secretion...
Proteomics has been little used for the identification of novel prognostic and/or therapeutic markers in isocitrate dehydrogenase (IDH)-wildtype glioblastoma (GB). In this study, we analyzed 50 tumor and 30 serum samples from short- long-term survivors IDH-wildtype GB (STS LTS, respectively) by data-independent acquisition mass spectrometry (DIA-MS)-based proteomics, with aim identifying such markers. DIA-MS identified 5422 826 normalized proteins samples, respectively, only three 26...
Abstract A simple and efficient method for monobromination of aromatic amines predominantly in the para position is reported. Tetrabutylammonium tribromide used at 20° yields parabromoanilines are higher than 90%.
Activated in response to chemotherapy, senescence is a tumor suppressive mechanism that induces permanent loss of proliferation. However, treatment, it not really known how cells can escape and irreversible or incomplete this pathway is. We have recently described are more transformed than non-treated parental cells, they resist anoikis rely on Mcl-1. In study, we further characterize emergence irinotecan, first line treatment used colorectal cancer. Our results indicate Akt was activated as...