A5100658238- Lymphoma Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Plant tissue culture and regeneration
- Adrenal Hormones and Disorders
- Porphyrin Metabolism and Disorders
- Hormonal Regulation and Hypertension
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Heat shock proteins research
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Genetics, Bioinformatics, and Biomedical Research
- Erythrocyte Function and Pathophysiology
- Chronic Lymphocytic Leukemia Research
- Race, Genetics, and Society
- Innovations in Medical Education
- Microbial Metabolites in Food Biotechnology
- Census and Population Estimation
- RNA and protein synthesis mechanisms
- thermodynamics and calorimetric analyses
- Neonatal Health and Biochemistry
- Postharvest Quality and Shelf Life Management
- Bee Products Chemical Analysis
- Mitochondrial Function and Pathology
- Polyamine Metabolism and Applications
William Harvey Research Institute
2023-2025
Queen Mary University of London
2022-2025
Phoenix (Italy)
2024
Australian Football League
2023-2024
Memorial University of Newfoundland
2022
Wellcome Sanger Institute
2022
Memorial
2022
VIR Biotechnology (United States)
2021
University of Michigan
2012-2016
Queen's University
2015
The enzymes cyclooxygenase-1 and cyclooxygenase-2 (COX-1 COX-2) catalyze the conversion of arachidonic acid to prostaglandin (PG) H2, precursor PGs thromboxane. These lipid mediators play important roles in inflammation pain normal physiological functions. While there are abundant data indicating that inducible isoform, COX-2, is pain, constitutively expressed COX-1, has also been suggested a role inflammatory processes. To address latter question pharmacologically, we used highly selective...
The MALDI-TOF spectra of peptides from the sera normal and myocardial infarction patients produced patterns that provided an accurate diagnostic MI. In infarction, spectral pattern originated cleavage complement C3 alpha chain to release C3f peptide fibrinogen A. A were in turn progressively truncated by aminopeptidases produce two families fragments formed characteristic Time course inhibitor studies demonstrated serum reflect balance disease-specific-protease aminopeptidase activity ex...
Fruits of Lycopersicon esculentum Mill cv Sonatine stored in 6% CO(2), O(2), and 88% N(2) for 14 weeks at 12 degrees C, exhibited a temporal separation certain biochemical events associated with ripening.The specific activity two citric acid cycle enzymes, citrate synthase malate dehydrogenase, fell substantially during the first 2 storage when changes organic concentration also occurred. During this period, lycopene, polygalacturonase, ethylene were undetectable.When fruit removed from...
Human colonic bacteria are necessary for the digestion of many dietary polysaccharides. The intestinal symbiont Bacteroides thetaiotaomicron uses five outer membrane proteins to bind and degrade starch. Here, we report x-ray crystallographic structures SusE SusF, two composed tandem starch specific carbohydrate-binding modules (CBMs) with no enzymatic activity. Examination CBMs in three SusF reveals subtle differences way each binds is reflected their K(d) values both high molecular weight...
ABSTRACT Polysaccharide utilization loci (PUL) within the genomes of resident human gut Bacteroidetes are central to metabolism otherwise indigestible complex carbohydrates known as “dietary fiber.” However, functional characterization PUL lags significantly behind sequencing efforts, which limits physiological understanding human-bacterial symbiosis. In particular, molecular basis polysaccharide recognition, an essential prerequisite hydrolysis by cell surface glycosidases and subsequent...
Heat shock proteins (Hsps) are known to be induced in response short-term stress. The present study aimed evaluate the potential of Hsp70 as a biomarker stress produced by increased temperature, osmotic pressure, and exposure cadmium sodium chloride marine macroalgae fresh water plant species. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed with working range 0.025–10 μg ml−1 using monoclonal antibody raised against purified Phaseolus aureus (mung bean)....
Glioblastoma Multiforme (GBM), the most common and lethal primary human brain tumor, exhibits multiple molecular aberrations. We report that loss of transcription factor GATA4, a negative regulator normal astrocyte proliferation, is driver in glioma formation fulfills hallmarks tumor suppressor gene (TSG). Although GATA4 was expressed brain, observed 94/163 GBM operative samples survival prognostic marker. occurred through promoter hypermethylation or novel somatic mutations. Loss astrocytes...
Abstract Background Studies of Primary Adrenal Insufficiency (PAI) in African children are rare but Sudan, congenital adrenal hyperplasia (CAH) and Triple A syndrome the most common genetic causes. Differential diagnosis is challenging, especially resource-limited settings, where presentation can mimic childhood diseases facilities for biochemical testing may be restricted. Patients & methods Forty-eight (48) patients from 43 families (31M:17F) with PAI were included (CAH/Triple...
The gingiva heals at an accelerated rate with reduced scarring when compared to skin. Potential well-studied factors include immune cell number, angiogenesis disparities and fibroblast gene expression. Differential keratinocyte expression, however, remains relatively understudied. This study explored the contrasting healing efficiencies of gingival skin keratinocytes, alongside their differential expression patterns.3D organotypic culture models human were developed using temporarily...
The use of cyclophosphamide-treated mice for purpose cloning the African trypanosomes was assessed. C3HeB/FeJ were injected with 200 mg/kg cyclophosphamide (CY) 24-72 hours prior to infection a single trypanosome isolated from Trypanosoma rhodesiense infected blood samples. All CY-treated exhibited depressed parasite-exposure, which period time sufficient grow organism fulminating parasitemia. Cloning efficiency routinely 45% in these animals. Thus, our study demonstrates that CT-treated are...
Heat shock proteins (HSP) play an important role in auto‐immunity and infection. Glutamic acid decarboxylase (GAD) the prime antigen Type 1 diabetes has similar amino sequences to HSP65. An ELISA was developed using a plant‐derived HSP65 antibody. antibody present serum of all normal subjects (median 1.64 AU, IQ range 1.49‐1.74). Lower levels were found established (1.41 1.32‐1.61, p <0.001) 2 (1.45 1.35‐1.59, <0.006). In HSP fell with age ( = 0.007) duration 0.008) women had lower...
Abstract Objective Adrenocortical carcinomas (ACCs) are invasive tumours arising in the adrenal cortex, and steroidogenic associated with worse prognostic outcomes. Loss-of-function mutations sphingosine-1-phosphate lyase (SGPL1) cause primary insufficiency as a key degradative enzyme sphingolipid pathway, SGPL1 also influences balance of pro-proliferative pro-apoptotic sphingolipids. We, therefore, hypothesized increased may be linked to disease severity ACC. Design Analyse expression...