A5015488181- Gut microbiota and health
- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Escherichia coli research studies
- Enzyme Production and Characterization
- Clostridium difficile and Clostridium perfringens research
- Dispute Resolution and Class Actions
- Advanced Fluorescence Microscopy Techniques
- Education Systems and Policy
- Law, Rights, and Freedoms
- Business Law and Ethics
- Legal Systems and Judicial Processes
- Genomics and Phylogenetic Studies
- Digital Economy and Work Transformation
- Medical Malpractice and Liability Issues
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Vibrio bacteria research studies
- Cell Image Analysis Techniques
- Legume Nitrogen Fixing Symbiosis
- Carbon and Quantum Dots Applications
- Law, AI, and Intellectual Property
- Genetics, Aging, and Longevity in Model Organisms
- Microbial Metabolism and Applications
- Cancer Risks and Factors
Western Washington University
2024
CooperSurgical (United States)
2022
Alma College
2007-2019
The University of Texas Southwestern Medical Center
2015-2019
University of Minnesota
2018-2019
George Washington University
2019
University of Michigan
2012-2015
Southwestern Medical Center
2015
Villanova University
2005
Australian Museum
1993
Human colonic bacteria are necessary for the digestion of many dietary polysaccharides. The intestinal symbiont Bacteroides thetaiotaomicron uses five outer membrane proteins to bind and degrade starch. Here, we report x-ray crystallographic structures SusE SusF, two composed tandem starch specific carbohydrate-binding modules (CBMs) with no enzymatic activity. Examination CBMs in three SusF reveals subtle differences way each binds is reflected their K(d) values both high molecular weight...
To compete for the dynamic stream of nutrients flowing into their ecosystem, colonic bacteria must respond rapidly to new resources and then catabolize them efficiently once they are detected. The Bacteroides thetaiotaomicron starch utilization system (Sus) is a model nutrient acquisition by symbiotic gut bacteria, which harbor thousands related Sus-like systems. Structural investigation four Sus outer membrane proteins (SusD, -E, -F, -G) revealed that contain total eight starch-binding...
ABSTRACT Gut microbes play a key role in human health and nutrition by catabolizing wide variety of glycans via enzymatic activities that are not encoded the genome. The ability to recognize process carbohydrates strongly influences structure gut microbial community. While effects diet on microbiota well documented, little is known about molecular processes driving metabolism. To provide mechanistic insight into carbohydrate catabolism symbionts, we studied starch processing real time model...
The gut microbiota is usually regarded as providing colonization resistance against enteric pathogens. However, some pathogens evolved to thrive with the aid of certain members microbiota. Several Gram-negative bacteria employ type three secretion systems (T3SSs), which are molecular syringes that deliver effector proteins host cells, hijacking cell function. Here we show T3SS enterohemorrhagic E. coli (EHEC) cleaved by self and microbiota-derived proteases. Self-cleavage limits...
The gut microbiota can significantly impact invading pathogens and the disease they cause; however, many of mechanisms that dictate commensal-pathogen interactions remain unclear. Enterohemorrhagic Escherichia coli (EHEC) is a potentially lethal human intestinal pathogen uses microbiota-derived molecules as cues to efficiently regulate virulence factor expression. Here, we investigate interaction between EHEC Enterococcus faecalis, common commensal, show E. faecalis affects both expression...
This paper proposes a solution to facilitate on-orbit satellite communication data integrity and security by utilizing blockchain technology. A proliferating demand has created growing reliance on satellites for communications, GPS, imagery, other uses. The decreased cost of entry into the space industry increased number entrants also amount debris. These orbital debris pose serious threat spacecraft. Therefore, tracking these objects is crucial. Blockchain technology offers unique this...
Single-molecule fluorescence (SMF) permits imaging with nanometer-scale resolution. This technique is particularly useful for cellular as it provides a non-invasive, minimally perturbative means to examine macromolecular localization and dynamics, even in live cells. Here, we demonstrate that SMF can be extended new category of experiments: intracellular live, obligate anaerobic cells on the benchtop. We investigate starch-utilization system (Sus) proteins gut symbiont <i>Bacteroides...
ABSTRACT The QseEF histidine kinase/response regulator system modulates expression of enterohemorrhagic Escherichia coli (EHEC) and Salmonella enterica serovar Typhimurium virulence genes in response to the host neurotransmitters epinephrine norepinephrine. qseG , which encodes an outer membrane lipoprotein, is cotranscribed with qseEF these enteric pathogens, but there little knowledge its role virulence. Here, we found that EHEC QseG interacts type III secretion (T3SS) gate protein SepL...
The quantity of Internet Things (IoT) devices in the marketplace and lack security is staggering. interconnectedness IoT has increased attack surface for hackers. "White Worm" technology potential to combat infiltrating malware. Before white worm becomes viable, its capabilities must be constrained specific limited non-harmful actions. This paper addresses current problem, international research, conflicting interest individuals, businesses, governments regarding technology. Proposed a new...
Transport of tritiated cyclic AMP in the coleoptile oats (Avena sativa) and corn (Zea mays) is polar, with basipetal to acropetal ratios 4.0 3.2, respectively. The rate transport approximately that indoleacetic acid. linear velocity transport, however, at least five times auxin. A loss polarity nucleotide occurs subapical tissues within several hours after decapitation coleoptile, accompanied by a decrease rate. not reversed exogenous auxin, but reduction is. Auxin also inhibits uptake AMP....
ABSTRACT Caenorhabditis elegans gut and cuticle produce a disruptive amount of autofluorescence during imaging. Although C. has been characterized, it not characterized at high resolution using both spectral fluorescence lifetime-based approaches. We performed scans whole, living animals to characterize adult elegans. By scanning 405 nm, 473 561 647 nm excitations, we produced profiles that confirm the brightest clear overlap with emission green fluorescent protein (GFP). then used lifetime...
Gerontological nurses must advocate for improved quality of nursing staff and remember the importance balancing cost in homes.
The use of restraints in health care facilities is necessary but fraught with regulatory dangers, patient safety risks and negative optics. Proper within guidelines can enhance outcomes, every must be viewed as a risk event for the facility professionals involved.