A5038777064- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cancer therapeutics and mechanisms
- DNA and Nucleic Acid Chemistry
- Synthesis and Biological Evaluation
- Cancer-related Molecular Pathways
- 14-3-3 protein interactions
- HIV Research and Treatment
- Biochemical and Molecular Research
- Wnt/β-catenin signaling in development and cancer
- Synthesis and Reactions of Organic Compounds
- Carcinogens and Genotoxicity Assessment
- Glycosylation and Glycoproteins Research
- Nanoparticles: synthesis and applications
- Genetic factors in colorectal cancer
- Fluorine in Organic Chemistry
- Galectins and Cancer Biology
- Epigenetics and DNA Methylation
- Gold and Silver Nanoparticles Synthesis and Applications
- Cellular transport and secretion
- PARP inhibition in cancer therapy
- Colorectal Cancer Treatments and Studies
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
University of South Carolina
2016-2025
University of South Carolina Sumter
2008-2023
South Carolina Department of Education
2014
Misr International University
2012
Wayne State University
2012
University of Pittsburgh
2012
University College London
1994-2003
Harvard University
1999-2000
University of Nebraska Medical Center
1997
Mario Negri Institute for Pharmacological Research
1995
A series of gold nanoparticles were examined for uptake and acute toxicity in human leukemia cells. The (average diameter=18 nm), which possessed various surface modifiers, not toxic to cells during continuous exposure three days. Citrate-capped further their cellular by absorbance transmission electron microscopy (see image). Results indicate that although some precursors may be toxic, the themselves are necessarily detrimental function. results Nanoscience nanotechnology hold great promise...
Gold nanorods of different aspect ratios are prepared using the growth-directing surfactant, cetyltrimethylammonium bromide (CTAB), which forms a bilayer on gold nanorod surface. Toxicological assays CTAB-capped solutions with human colon carcinoma cells (HT-29) reveal that apparent cytotoxicity is caused by free CTAB in solution. Overcoating polymers substantially reduces cytotoxicity. The number taken up per cell, for surface coatings, quantitated inductively coupled plasma mass...
The DNA binding properties of a series imidazole-containing and C-terminus-modified analogues 4-7 distamycin are described. These contain one to four imidazole units, respectively. Data from the ethidium displacement assay showed that these compounds bind in minor groove DNA, with relative order constants 6 (Im3) > 7 (Im4) 5 (Im2) 4 (Im1). reduced for poly(dA-dT) distamycin, while they still interact strongly poly(dG-dC), provided evidence GC sequence acceptance. preferences GC-rich...
3-methyladenine (3MeA) DNA glycosylases remove 3MeAs from alkylated to initiate the base excision repair pathway. Here we report generation of mice deficient in 3MeA glycosylase encoded by Aag ( Mpg ) gene. Alkyladenine turns out be major not only for cytotoxic lesion, but also mutagenic 1, N 6 -ethenoadenine (ɛA) and hypoxanthine lesions. appears liver, testes, kidney, lung, ɛA kidney; another may expressed lung. Although alkyladenine has capacity 8-oxoguanine lesions, it does appear...
The human 3-methyladenine DNA glycosylase [alkyladenine (AAG)] catalyzes the first step of base excision repair by cleaving damaged bases from DNA. Unlike other glycosylases that are specific for a particular type base, AAG excises chemically diverse selection substrate alkylation or deamination. 2.1-Å crystal structure complexed to containing 1, N 6 -ethenoadenine suggests how modified can be distinguished normal in enzyme active site. Mutational analyses residues contacting alkylated...
Aim: The authors have systematically investigated the anticancer potentiality of silver-based nanoparticles (AgNPs) and mechanism underlying their biological activity in human colon cancer cells. Materials & methods: Starch-capped AgNPs were synthesized, characterized evaluated through multiple biochemical assays. Results: decreased growth viability HCT116 AgNP exposure increased apoptosis, as demonstrated by an increase 4´,6-diamidino-2-phenylindole-stained apoptotic nuclei, BAX/BCL-XL...
Abstract The small molecule Quinacrine (QC, a derivative of 9‐aminoacridine), an anti‐malaria drug, displays activity against cancer cell lines and can simultaneously suppress nuclear factor‐κB (NF‐κB) activate p53 signaling. In this study, we investigated the anticancer mechanism underlying these drug activities in breast lines. QC caused dose‐dependent decrease both anchorage dependent independent growth cells (MCF‐7 MDA‐MB‐231) without affecting normal epithelial (MCF‐10A), as evident...
Smoking is the largest preventable cause of death and disease in United States. However, <5% quit attempts are successful, underscoring urgent need for novel therapeutics. Microglia one untapped therapeutic target. While previous studies have shown that microglia mediate both inflammatory responses brain plasticity, little known regarding their role nicotine dependence withdrawal phenotypes. Here, we examined microglial changes striatum-a mesolimbic region implicated rewarding effects drugs...
We previously showed that quinacrine (QC), a small molecule antimalarial agent, also presented anticancer activity in breast cancer cells through activation of p53, p21, and inhibition topoisomerase activity. Here we have systematically studied the detailed cell death mechanism this drug using three colon lines (HCT-116 parental, isogenic HCT-116 p53-/-, p21-/- sublines). QC caused dose-dependent reduction viability all lines. However, parental were more susceptible to QC-mediated death,...
Gold nanorods were synthesized using a seed-mediated wet chemical approach with quaternary ammonium surfactant, cetyltrimethylammonium bromide (CTAB), that forms bilayer on the surface of nanorods. The CTAB molecules in exchanged similar polymerizable analog, 11-(acryloyloxy) undecyltrimethyl (p-CTAB). Mass spectrometric analysis degree exchange for p-CTAB, after gold digestion, gave 77 ± 3 and 23 1% p-CTAB CTAB, respectively. On-rod polymerization cationic free-radical initiator was...
We previously reported that quinacrine (QC) has anticancer activity against breast cancer cells. Here, we examine the mechanism of action QC and its ability to inhibit Wnt-TCF signaling in two independent cell lines. altered components by increasing levels adenomatous polyposis coli (APC), DAB2, GSK-3β axin decreasing β-catenin, p-GSK3β (ser 9) CK1. also reduced Wnt transcription factor TCF/LEF downstream targets cyclin D1 c-MYC. Using a luciferase-based assay, it was shown APC were...
Cannabidiol (CBD) is a natural product associated with wide range of biological and therapeutic activities. Despite the widespread cultural acceptance CBD as medicinal agent, much remains to be determined regarding its precise mechanism(s) action in treating multiple conditions. has been shown promiscuously interact several neurological targets varying affinities. To expand chemical space phytocannabinoids develop novel compounds, we have designed synthesized series Δ8-THC homodimers,...
Journal Article DNA sequence-specific adenine alkylation by the novel antitumor drug tallimustine (FCE 24517), a benzoyl nitrogen mustard derivative of distamycin Get access Massimo Broggini, Broggini Search for other works this author on: Oxford Academic PubMed Google Scholar Helen M. Coley, Coley + Present address: Institute Cancer Research, Section Drug Development, 15 Cotswold Road. Belmont, Sutton, Surrey SM2 5NG, UK Nicola Mongelli, Mongelli 1Pharmacia-Farmitalia Carlo ErbaNerviano,...
When expressed via an inducible promoter in human embryonic kidney 293 cells, the rat Mas-related gene D (rMrgD) receptor responded to β-alanine but not l-alanine by elevating intracellular [Ca<sup>2+</sup>], stimulating phosphorylation of mitogenactivated protein kinases known as extracellular signal-regulated kinase (ERK) 1 and ERK2 translocating from plasma membrane punctate vesicles. By contrast, related E (rMrgE) did respond β-alanine. Coexpression rMrgD with rMrgE, which occurs...