The aim of this study was to determine the mechanisms curcumin-induced human breast cancer cell apoptosis. From quantitative image analysis data showing an increase in percentage cells with a sub-G0/G1 DNA content, we demonstrated apoptosis line MCF-7, which expression wild-type p53 could be induced. Apoptosis accompanied by level as well its DNA-binding activity followed Bax at protein level. Further experiments using p53-null MDAH041 low and high p53-expressing TR9-7 cell, is under tight...

Curcumin (diferuloylmethane) is known to induce apoptosis in tumor cells. In asynchronous cultures, with time-lapse video-micrography combination quantitative fluorescence microscopy, we have demonstrated that curcumin induces at G2 phase of cell cycle deregulated cyclin D1-expressed mammary epithelial carcinoma cells, leaving its normal counterpart unaffected. our search toward delineating the molecular mechanisms behind such differential activities curcumin, found it selectively increases...

Aim: The authors have systematically investigated the anticancer potentiality of silver-based nanoparticles (AgNPs) and mechanism underlying their biological activity in human colon cancer cells. Materials & methods: Starch-capped AgNPs were synthesized, characterized evaluated through multiple biochemical assays. Results: decreased growth viability HCT116 AgNP exposure increased apoptosis, as demonstrated by an increase 4´,6-diamidino-2-phenylindole-stained apoptotic nuclei, BAX/BCL-XL...

Abstract The small molecule Quinacrine (QC, a derivative of 9‐aminoacridine), an anti‐malaria drug, displays activity against cancer cell lines and can simultaneously suppress nuclear factor‐κB (NF‐κB) activate p53 signaling. In this study, we investigated the anticancer mechanism underlying these drug activities in breast lines. QC caused dose‐dependent decrease both anchorage dependent independent growth cells (MCF‐7 MDA‐MB‐231) without affecting normal epithelial (MCF‐10A), as evident...

Breast cancer is considered as an increasing major life-threatening concern among the malignancies encountered globally in females. Traditional therapy far from satisfactory due to drug resistance and various side effects, thus a search for complementary/alternative medicines natural sources with lesser effects being emphasized. Andrographis paniculata, oriental, traditional medicinal herb commonly available Asian countries, has long history of treating variety diseases, such respiratory...

We previously showed that quinacrine (QC), a small molecule antimalarial agent, also presented anticancer activity in breast cancer cells through activation of p53, p21, and inhibition topoisomerase activity. Here we have systematically studied the detailed cell death mechanism this drug using three colon lines (HCT-116 parental, isogenic HCT-116 p53-/-, p21-/- sublines). QC caused dose-dependent reduction viability all lines. However, parental were more susceptible to QC-mediated death,...

Lung cancer is the leading cause of cancer-related deaths worldwide. Despite decades research, treatment options for lung patients remain inadequate, either to offer a cure or even substantial survival advantage owing its intrinsic resistance chemotherapy. Our results propose effectiveness capsaicin in down-regulating VEGF expression non-small cell carcinoma (NSCLC) cells hypoxic environment. Capsaicin-treatment re-activated p53-SMAR1 positive feed-back loop these persuade p53-mediated...

We previously reported that quinacrine (QC) has anticancer activity against breast cancer cells. Here, we examine the mechanism of action QC and its ability to inhibit Wnt-TCF signaling in two independent cell lines. altered components by increasing levels adenomatous polyposis coli (APC), DAB2, GSK-3β axin decreasing β-catenin, p-GSK3β (ser 9) CK1. also reduced Wnt transcription factor TCF/LEF downstream targets cyclin D1 c-MYC. Using a luciferase-based assay, it was shown APC were...

ABSTRACT Kaposi's sarcoma-associated herpesvirus (KSHV) DNA persists in latently infected cells as an episome via tethering to the host chromosomes. The latency-associated nuclear antigen (LANA) of KSHV binds cis- acting elements terminal repeat (TR) region genome through its carboxy terminus. Previous studies have demonstrated that LANA is important for maintenance and replication TR-containing plasmids. Here we report associates with origin recognition complexes (ORCs) when bound 17-bp...

Epstein-Barr virus (EBV) infects most of the human population and persists in B lymphocytes for lifetime host. The establishment latent infection by EBV requires expression a unique repertoire genes. product one these viral genes, nuclear antigen 3C (EBNA3C), is essential growth transformation primary vitro can regulate transcription number cellular genes important immortalization process. This study demonstrates an associated function EBNA3C which involves disruption G2/M cell cycle...

Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus associated with the development of both lymphoid and epithelial tumors. The EBV critical latent antigens EBNA1 EBNA3C interact Nm23-H1, known suppressor cell migration tumor metastasis. This interaction for regulation downstream cellular genes involved in tumorigenesis migration. significance these interactions was determined nude mice using cancer cells expressing Nm23-H1. promoted growth transformed vivo, but their expression less...

Resveratrol (Res) can modulate multiple cellular pathways relevant for tumorigenesis but is less effective in colon cancer compared to breast cancer. To increase the chemopreventive potential of Res combination with 5-fluorouracil (5-FU), a systematic study was carried out cells. HCT-116 cells were treated and 5-FU several cell-based assays, such as MTT, clonogenic, wound healing, DAPI, comet assay, Western blot, performed. A significant inhibition cell proliferation, migration, increased...

AbstractNm23-H1 is a well-known tumor metastasis suppressor, which functions as nucleoside-diphosphate kinase converting nucleoside diphosphates to triphosphates with an expense of ATP. It regulates variety cellular activities, including proliferation, development, migration and differentiation known be modulated by series complex signaling pathway. Few studies have addressed the mechanistic action Nm23-H1 in context these processes. To determine downstream pathways Nm23-H1, we expressed...

Previous studies have demonstrated the interaction between Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA3C) and metastatic suppressor Nm23-H1 both in vitro vivo (C. Subramanian, M. A. Cotter II, E. S. Robertson, Nat. Med. 7:350-355, 2001). EBNA3C can reverse ability of to suppress migration Burkitt's lymphoma breast carcinoma cell lines vitro. contributes EBV-associated human cancers by regulating transcription a number cellular viral promoters targeting altering activities metastasis...

Kaposi's sarcoma-associated herpesvirus (KSHV) is a human tumor virus expressing latent antigens critical for pathogenesis. The mechanism by which KSHV mediates oncogenesis has not been fully elucidated. Notch signaling an evolutionarily conserved pathway controlling diverse events related to development, proliferation, and tissue homeostasis. Deregulation of also shown be highly correlated with oncogenesis. Here we show that the activated intracellular domain Notch1 (ICN) aberrantly...