A5101955450- Gut microbiota and health
- MicroRNA in disease regulation
- Infant Nutrition and Health
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Inflammatory Bowel Disease
- Glycosylation and Glycoproteins Research
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Venous Thromboembolism Diagnosis and Management
- Circular RNAs in diseases
- Microscopic Colitis
- Soil Carbon and Nitrogen Dynamics
- Bladder and Urothelial Cancer Treatments
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Urinary and Genital Oncology Studies
- Helicobacter pylori-related gastroenterology studies
- Neuroscience and Neuropharmacology Research
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- IL-33, ST2, and ILC Pathways
- Cancer Research and Treatments
- Systemic Lupus Erythematosus Research
Nantong University
2025
Wuxi No.2 People's Hospital
2025
Xuzhou Medical College
2025
China State Construction Engineering (China)
2025
First Affiliated Hospital of Jinan University
2024
Materials Science & Engineering
2024
Chongqing University
2022-2024
University of Jinan
2024
Shanghai Jiao Tong University
2013-2023
Luliang University
2023
Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth beneficial intestinal bacteria, receptor analogues inhibit binding pathogens, and substances that promote postnatal brain development. There is further evidence HMOs participate in modulating human immune system. Because absorption, catabolism, biological function (OS) have strong correlations with their structures, structure elucidation key advancing this research....
Sialylated human milk oligosaccharides (SHMOs) are important components of oligosaccharides. Sialic acids typically found on the nonreducing end and known binding sites for pathogens aid in neonates' brain development. Due to their negative charge hydrophilic nature, they also help modulate cell-cell interactions. It has been shown that sialic involved regulating immune response In this study, enriched SHMOs from pooled sample were analyzed by HPLC-Chip/QTOF MS. The instrument employs a...
ABSTRACT Bifidobacterium longum subsp. infantis ATCC 15697 utilizes several small-mass neutral human milk oligosaccharides (HMOs), of which are fucosylated. Whereas previous studies focused on endpoint consumption, a temporal glycan consumption profile revealed time-dependent effect. Specifically, among preferred HMOs, tetraose was favored early in fermentation, with other consumed slightly later. In order to utilize fucosylated oligosaccharides, possesses fucosidases, implicating GH29 and...
ABSTRACT Objectives: This study tested the hypothesis that fecal bacterial genera of breast‐fed (BF) and formula‐fed (FF) infants differ human milk oligosaccharides (HMOs) modulate microbiota BF infants. Methods: Fecal samples were obtained from (n = 16) or FF 6) at 3‐month postpartum. Human collected on same day when feces collected. The was assessed by pyrosequencing 16S ribosomal RNA genes. HMOs measured high‐performance liquid chromatography–chip time‐of‐flight mass spectrometry....
Several studies suggest that mesenchymal stem cells (MSCs) possess antitumor properties; however, the exact mechanisms remain unclear. Recently, microvesicles (MVs) are considered as a novel avenue intercellular communication, which may be mediator in MSCs-related effect. In present study, we evaluated whether MVs derived from human umbilical cord Wharton's jelly (hWJMSCs) inhibit bladder tumor T24 growth using cell culture and BALB/c nu/nu mice xenograft model. CCK-8 assay Ki-67...
Human milk oligosaccharides (HMOs), as an abundant and bioactive component of breast milk, work in many ways to promote the health fed infants. The expression HMOs has been shown vary accordance with Lewis blood type secretor status, women different types differ α1,2 fucosyltransferase (FUT2) α1,3/4 (FUT3). In this study, were extracted from 60 Gambia, Africa various types. profiled using high resolution HPLC-Chip/TOF mass spectrometry. Notably, amounts fucosylation varied significantly...
Lactating mothers secrete milk sialyloligosaccharides (MSOs) that function as anti-adhesives once provided to the neonate. Particular infant-associated commensals, such Bifidobacterium longum subsp. infantis, consume neutral oligosaccharides, although their ability utilize acidic oligosaccharides has not been assessed. Temporal glycoprofiling of HMO consumed during fermentation demonstrated a single composition, with several isomers, corresponding sialylated lacto-N-tetraose. To MSO, B....
Roseburia intestinalis (R. intestinalis) is one of the dominant intestinal bacterial microbiota and decreased in patients with inflammatory bowel disease (IBD). It helps protect colonic mucosa against development inflammation subsequent IBD, however its underlying mechanisms are unclear. The aim present study was to evaluate anti‑inflammatory properties R. vitro an animal model IBD. effects on activity index (DAI) scores, pathology, expression interleukin (IL)‑17 frequency CD4+CD25+Foxp3+...
The study aims to elucidate the anti-inflammatory effect and mechanism of Roseburia intestinalis (R. intestinalis) in Crohn's disease (CD).16S-rRNA genome sequencing technique is used detect characteristics intestinal microbiota untreated CD patients healthy controls. Then investigates effects R. on activity index score, pathology, differentiation Treg cells, expressions Thymic stromal lymphopoietin (TSLP), TGF-β IL-10 by using TNBS colitis models. At cellular level, uses LPS stimulate...
BackgroundCrohn's disease (CD) is a chronic inflammatory disorder characterized by intestinal immune dysfunction. Multiple factors, including gut dysbiosis, are involved in the pathogenesis of CD. However, effect commensal bacteria on controlling response individuals with CD remains unclear.MethodsWe detected Toll-like receptor 2 (TLR2), TLR4, and TLR5 expression Roseburia intestinalis (R. intestinalis)-treated mice 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Then, we...
In our previous study, microvesicles (MVs) released from human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) retard the growth of bladder cancer cells. We would like to know if MVs have a similar effect on renal cell carcinoma (RCC). By use culture and BALB/c nu/nu mice xeno-graft model, influence upon aggressiveness RCC (786-0) was assessed. Cell counting kit-8 (CCK-8) assay, incidence tumor, tumor size, Ki-67 or TUNEL staining used evaluate in vitro vivo. Flow cytometry assay (in...
The interleukin-17 (IL-17) cytokine family is crucial to the progression of experimental autoimmune encephalomyelitis (EAE), an animal model multiple sclerosis (MS). It has been shown in a neuroectoderm-specific knockout study that astrocyte-restricted ablation Act1, key and common transcription factor for signals mediated by IL-17 members (IL-17A, IL-17F, IL-17C), ameliorates EAE. However, effect Act1 deficiency astrocytes on ongoing disease, which clinical relevance MS therapy, not...
Abstract Background Small cohort studies have suggested oral tongue squamous cell carcinoma (OTSCC) could be associated with worse prognosis in individuals younger than 40. Methods We compared the survival of all OTSCC cases National Cancer Database under 40 years old those older 40, excluding patients over 70. Cox regression and propensity score matched (PSM) analyses were performed. Results A total 22 930 identified. The group consisted 2566 (9.9%) cases; 20664 to 70 (90.1%). Most male (13...
Abstract IFN-γ, the hallmark cytokine of Th1 cells, plays an important role in experimental autoimmune encephalomyelitis (EAE), animal model multiple sclerosis. Thus far, IFN-γ EAE has been largely studied through its effects on immune whereas much less is known about CNS especially vivo. In this study, we dissected vivo and mechanisms binding/signaling astrocytes microglia, found that signaling these cell types opposite pathogenesis. Silencing alleviated EAE, likely some infiltrating...
Inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's (CD), has a complex etiology that may be associated with dysbiosis of the microbiota. Previously, our study revealed significant loss Roseburia intestinalis from gut untreated patients CD, R. exerted anti‑inflammatory functions in TNBS‑induced colitis; however, function supernatant is unknown. Therefore, LPS‑induced macrophages, including RAW264.7 macrophages bone marrow‑derived were treated supernatant. The...
Growing evidence suggests that obesity is associated with the susceptibility and disease severity of multiple sclerosis. The chronic inflammation induced by believed to contribute this process. However, immune mechanisms connecting prevalence pathogenesis MS are poorly defined. In study, we show high fat diet (HFD)-induced obese mice developed an exacerbated EAE as indicated higher clinical scores more severe pathological changes in spinal cord than control fed normal (ND), following...
The therapeutic potential of adult neural stem cells (aNSCs) has been shown in EAE, an animal model MS, administered by either i.c.v. or i.v. injection. However, is invasive approach, while the route aNSCs associated with a non-specific immune suppression periphery. Here we demonstrate that intranasal (i.n.) delivery fluorescently labeled resulted their appearance olfactory bulb, cortex, hippocampus, striatum, brainstem, and spinal cord. These induce functional recovery from ongoing EAE...