A5074750666- Marine animal studies overview
- Physiological and biochemical adaptations
- Mercury impact and mitigation studies
- Isotope Analysis in Ecology
- Aquaculture Nutrition and Growth
- Cephalopods and Marine Biology
- Turtle Biology and Conservation
- Neuroscience of respiration and sleep
- Bat Biology and Ecology Studies
- Ichthyology and Marine Biology
- Zebrafish Biomedical Research Applications
- Aquaculture disease management and microbiota
- Metabolomics and Mass Spectrometry Studies
- Toxic Organic Pollutants Impact
- Advanced Chemical Sensor Technologies
- Cardiovascular and Diving-Related Complications
- Marine and fisheries research
- Avian ecology and behavior
- High Altitude and Hypoxia
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Immune Response and Inflammation
- Marine Bivalve and Aquaculture Studies
- Fatty Acid Research and Health
- Paraoxonase enzyme and polymorphisms
University of Alaska Fairbanks
2015-2024
Alaska Department of Fish and Game
2009-2020
National Oceanic and Atmospheric Administration
2009
NOAA National Marine Fisheries Service
1998-2009
NOAA National Marine Fisheries Service Alaska Fisheries Science Center
1998-2009
NOAA National Marine Fisheries Service Northwest Fisheries Science Center
2009
University of Central Florida
2007-2009
Denver Federal Center
2008
United States Geological Survey
2008
University of British Columbia
1996-2007
Abstract The determination of FAME by GC is among the most commonplace analyses in lipid research. Quantification with FID has been effectively performed for some time, whereas detection MS used chiefly qualitative analysis FAME. Nonetheless, sensitivity and selectivity methods advocate a quantitative role GC‐MS analysis—an approach that would be particularly advantageous complex biological samples, where spectrometric confirmation analytes advisable. To assess utility quantification,...
Measurements of body mass, composition, and O₂ consumption rate V̇o2 were used to quantify some the factors that influence metabolism during first 4 mo development in northern elephant seal pup. During 4-wk nursing period, average mass pups increased from 42.0 ± 3.5 kg 127.3 15.9 kg. By end 10-wk postweaning fast mean declined 83.1 1.5 Body fat 4% near birth approximately 48% at weaning but remained relatively constant throughout fast. Dramatic changes composition such as these would be...
Abstract Climate change-driven alterations in Arctic environments can influence habitat availability, species distributions and interactions, the breeding, foraging, health of marine mammals. Phocine distemper virus (PDV), which has caused extensive mortality Atlantic seals, was confirmed sea otters North Pacific Ocean 2004, raising question whether reductions ice could increase contact between sub-Arctic mammals lead to viral transmission across Ocean. Using data on PDV exposure infection...
Although breath holding during diving has been studied extensively in seals, the recent observation that these mammals also exhibit long-duration apnea while apparently sleeping not systematically examined. This project examined sleep northern elephant seal pups (Mirounga angustirostris). The animals exhibited a sequential pattern of wakefulness-slow-wave (SWS)-rapid eye movement (REM) resembled normal mammalian sleep. typical respiratory 4-mo-old consisted short periods continuous breathing...
Abstract Steller sea lions ( SSL ; Eumetopias jubatus ) grow their vibrissae continually, providing a multiyear record suitable for ecological and physiological studies based on stable isotopes. An accurate age‐specific growth rate is essential registering chronology along the length of record, interpreting timing ecologically important events. We utilized four methods to estimate in fetal, rookery pup, young‐of‐the‐year YOY ), yearling, subadult, adult . The majority were collected from...
Steller sea lion (Eumetopias jubatus) tissue mercury concentrations increasingly above thresholds of concern occur in regions Alaska where lack population recovery is noted. Selenium-monomethylmercury interactions may mitigate toxicosis but also result functional selenium deficiency, impacting essential selenium-dependent processes. Physiologically driven differences (organotropism) total ([THg]), ([TSe]), and monomethylmercury ([MeHg+ ]) confound interpretation for various age cohorts....
The development of heart rate and respiratory patterns related to sleep-associated apnea were studied in northern elephant seal (Mirounga angustirostris) pups adult males. Heart became more refined conjunction with an age-related increase duration pups. That is, older showed significant sinus arrhythmia, while breathing apneic both stable similar magnitude the arrhythmia minimum. By contrast, younger poor or nonexistent shorter durations a variable and/or high during apnea. Apnea was...
Abstract A number of techniques are available for the extraction lipids from a variety tissues; however, conventional methods characteristically labor intensive, typically involve large volumes toxic solvents, and usually require at least 1 g tissue. With availability accelerated solvent (ASE) technology, opportunity exists to modify classical lipid such that automated high‐pressure, high‐temperature extractions may be performed with use far smaller costly harmsul solvents. Moreover, high...
A new approach to the study of free-ranging, endangered western stock Steller sea lions (Eumetopias jubatus) was implemented at Alaska SeaLife Center in Seward, Alaska. Groups up four juvenile (n = 16) were held temporary quarantine for research periods three months. Hematological and blood chemistry parameters collected beginning end captivity compared free-ranging controls determine if animals can provide accurate physiological data representative their wild counterparts. Free-ranging pups...
We evaluated the plasma concentration of acute phase protein haptoglobin (Hp) from Steller sea lions (Eumetopias jubatus) and harbor seals (Phoca vitulina) in regions Alaska (USA) where populations these pinnipeds were declining compared values with concentrations Hp same species areas stable. Samples collected 1992 through 1994 at sites Southeast Alaska, Prince William Sound, Gulf Aleutian Islands. Significantly higher levels found samples decline to those stable populations. Based on...
Nine captive Steller sea lions ( Eumetopias jubatus (Schreber, 1776), 1.75–6 years of age) were fasted for 7–14 d to test the effect short-term fasting on changes in body mass and condition. Trials repeated during both summer breeding season nonbreeding seven animals elucidate whether there was a seasonal component ability adapt limited food resources. Mean percent loss per day higher juveniles (1.8% ± 0.2%·d –1 ) than subadults (1.2% 0.1%·d ), but no significant age-related differences...
Knowledge of diet–tissue stable isotope discrimination is required to properly interpret values and identify possible diet shifts, such as might be expected from nursing through weaning. This study compared δ 13 C 15 Ν οf paired serum vibrissal roots with those ingested milk (n = 52) free-ranging Steller sea lion ( Eumetopias jubatus (Schreber, 1776)) pups (1–11 months) juveniles (14–27 estimate discrimination. Mean N enrichment was 2.1‰ ± 0.6‰ at the root vibrissae (representing current...
Abstract We evaluated the Precision Xtra™ ketometer as part of a larger study categorizing fasting status free-ranging Steller sea lion (Eumetopias jubatus; SSL) pups which necessitated identification plasma β-hydroxybutyrate concentrations ([β-HBA]) around threshold <0.3 and ≥0.3 mmol/l. Whole blood samples mixed with sodium heparin (NaHep) or ethylenediaminetetraacetic acid liquid anticoagulants were tested <10 minutes after collection (n = 14; triplicate technical...
Four Steller sea lions (Eumetopias jubatus) aged 6 weeks were fasted for 2.5 d to determine how young pups mobilize energy reserves during short periods of fasting similar those experienced in the wild. At age, lost 5.1 ± 0.3% their body mass durin g2do f fasting, with an average daily loss 0.7 0.1 kg·d -1 . Plasma blood urea nitrogen (BUN) concentration increased significantly from 3.0 mM, after over- night fast, 4.8 0.5 fasting. It is apparent that BUN levels are quickly depressed, since...