A5038060499- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Bacterial Identification and Susceptibility Testing
- Neutropenia and Cancer Infections
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Streptococcal Infections and Treatments
- Biosensors and Analytical Detection
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Neonatal and Maternal Infections
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- Inflammasome and immune disorders
- SARS-CoV-2 detection and testing
- Gastrointestinal motility and disorders
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Sepsis Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced biosensing and bioanalysis techniques
Imperial College Healthcare NHS Trust
2016-2025
Imperial College London
2012-2025
North West London Pathology
2021-2025
Charing Cross Hospital
2018-2025
Hammersmith Hospital
2013-2024
St Mary's Hospital
2016-2024
NIHR Imperial Biomedical Research Centre
2020-2024
National Institute for Health Research
2019-2024
St. Mary's Hospital
2016-2023
National Health Service
2019-2022
The emergence of the Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) has required an unprecedented response to control spread infection and protect most vulnerable within society. Whilst pandemic focused society on threat emerging infections hand hygiene, certain antimicrobial stewardship policies may have be relaxed. It is unclear whether unintended consequences these changes will a net-positive or -negative impact rates resistance. urgent focus must controlling this pandemic,...
The COVID-19 pandemic is a global health emergency characterized by the high rate of transmission and ongoing increase cases globally. Rapid point-of-care (PoC) diagnostics to detect causative virus, SARS-CoV-2, are urgently needed identify isolate patients, contain its spread guide clinical management. In this work, we report development rapid PoC diagnostic test (<20 min) based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) semiconductor technology for detection...
The intestine is the primary colonisation site for carbapenem-resistant Enterobacteriaceae (CRE) and serves as a reservoir of CRE that cause invasive infections (e.g. bloodstream infections). Broad-spectrum antibiotics disrupt resistance mediated by gut microbiota, promoting expansion within intestine. Here, we show antibiotic-induced reduction microbial populations leads to an enrichment nutrients depletion inhibitory metabolites, which enhances growth. Antibiotics decrease abundance...
Virulence plasmids are associated with hypervirulent types of Klebsiella pneumoniae, which generally do not carry antibiotic resistance genes. In contrast, nosocomial isolates often resistance, but rarely virulence plasmids. Here, we describe in "high-risk" clones sequence (STs) 15, 48, 101, 147 and 383 carrying carbapenemase The whole genome sequences were determined by long-read nanopore sequencing. 12 all contained hybrid containing both All carried rmpA/rmpA2 the aerobactin cluster, two...
Abstract Fecal microbiota transplantation (FMT) yields variable intestinal decolonization results for multidrug-resistant organisms (MDROs). This study showed significant reductions in antibiotic duration, bacteremia, and length of stay 20 patients colonized/infected with MDRO receiving FMT (compared pre-FMT history, a matched group not FMT), despite modest rates.
Carbapenemase-producing Enterobacteriaceae (CPE) are emerging worldwide, limiting therapeutic options. Mutational and plasmid-mediated mechanisms of colistin resistance have both been reported. The emergence clonal spread was analysed in 40 epidemiologically-related NDM-1 carbapenemase producing Klebsiella pneumoniae isolates identified during an outbreak a group London hospitals. Isolates from July 2014 to October 2015 were tested for susceptibility using agar dilution, characterised by...
Abstract The increasing prevalence of antimicrobial resistance is a serious threat to global public health. One the most concerning trends rapid spread Carbapenemase-Producing Organisms (CPO), where colistin has become last-resort antibiotic treatment. emergence resistance, including mobilized ( mcr ) genes, raises possibility untreatable bacterial infections and motivates development improved diagnostics for detection colistin-resistant organisms. This work demonstrates response detecting...
We examined community- and hospital-acquired bloodstream infections (BSIs) in coronavirus disease 2019 (COVID-19) non-COVID-19 patients across 2 epidemic waves.We analyzed blood cultures of presenting to a London hospital group between January 2020 February 2021. reported BSI incidence, changes sampling, case mix, healthcare capacity, COVID-19 variants.We identified 1047 BSIs from 34 044 cultures, including 653 (62.4%) community-acquired 394 (37.6%) hospital-acquired. Important pattern were...
In Streptococcus cremoris SK11, different permutations of a total 8 plasmids were observed within and between cultures various origins. All showed similar growth rates in milk. Those variants which carried 34-MDa plasmid, pSK112, resistant to bacteriophage øSK11G, whereas those from the plasmid was absent or had been cured sensitive this phage. Plasmid pSK112 shown confer resistance by reduced phage adsorption. These observations have important potential for development phage-resistant dairy...
ABSTRACT Sepsis is now the leading direct cause of maternal death in United Kingdom, and Streptococcus pyogenes pathogen. We combined conventional genomic analyses to define duration scale a lethal outbreak. Two postpartum deaths caused by S. occurred within 24 h; one was characterized bacteremia shock other hemorrhagic pneumonia. The women gave birth minutes each same maternity unit 2 days earlier. Seven additional infections health care household contacts were subsequently detected...
The gut microbiome can be adversely affected by chemotherapy and antibiotics prior to hematopoietic cell transplantation (HCT). This affects graft success increases susceptibility multidrug-resistant organism (MDRO) colonization infection. We performed an initial retrospective analysis of our use fecal microbiota (FMT) from healthy donors as therapy for MDRO-colonized patients with hematological malignancy. FMT was on eight pre-HCT (FMT-MDRO group), outcomes compared 11 MDRO colonized HCT...
In response to infection with New Delhi metallo-beta-lactamase (NDM)-producing Enterobacterales, combination antimicrobial therapy ceftazidime/avibactam (CAZ/AVI) plus aztreonam (ATM) has been explored. This study evaluated a practical laboratory method of testing for clinically significant synergy between CAZ/AVI+ATM in NDM-producing Enterobacterales.Minimum inhibitory concentrations (MICs) clinical isolates were determined ATM alone and using broth dilution. Restoration the breakpoint...
Carbapenemase-producing Enterobacterales (CPE) are challenging in healthcare, with resistance to multiple classes of antibiotics. This study describes the emergence IMP-encoding CPE amongst diverse species between 2016 and 2019 across a London regional network.
Early and accurate treatment of infections due to carbapenem-resistant organisms is facilitated by rapid diagnostics, but rare resistance mechanisms can compromise detection. One year after a Guiana Extended-Spectrum (GES)-5 carbapenemase-positive Klebsiella oxytoca infection was identified whole-genome sequencing (WGS; later found be part cluster 3 cases), 11 patients with GES-5-positive K. over 18 weeks in the same hospital.Bacteria were matrix-assisted laser desorption/ionization-time...
Rapid and accurate identification of patients colonised with carbapenemase-producing organisms (CPOs) is essential to adopt prompt prevention measures reduce the risk transmission. Recent studies have demonstrated ability combine machine learning (ML) algorithms real-time digital PCR (dPCR) instruments increase classification accuracy multiplex assays when using synthetic DNA templates. We sought determine if this novel methodology could be applied improve five major carbapenem-resistant...
ObjectivesSince May 2022, cases of human monkeypox virus (hMPXV) with human-to-human cross-transmission have significantly increased in nonendemic countries. Our aim was to characterize diagnostic features patients confirmed and possible guide future risk stratification describe a virtual care model.MethodsWe performed retrospective case-control study 140 assessed screened for suspected monkeypox; on hMPXV polymerase chain reaction testing, 70 were positive, negative. Data compared generate...
Enterobacteriaceae are a common cause of hospital infections. Carbapenems clinically effective treatment such However, resistance is on the rise. In particular, carbapenemase-producing carbapenem-resistant (CP-CRE) increasingly common. order to limit spread in clinical settings, screening and isolation being recommended, but many different methods available. We aimed compare impact costs three algorithms for detecting CP-CRE carriage. developed an individual-based simulation model using data...
Invasive Group B streptococcus (GBS) is a major cause of serious neonatal infection. Current strategies to reduce early-onset GBS disease have no impact on late-onset (LOD). Although LOD viewed as sporadic event in the community, arising within intensive care unit (ICU) raises questions about mode acquisition.