A5041529549- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Pharmaceutical and Antibiotic Environmental Impacts
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Escherichia coli research studies
- Infective Endocarditis Diagnosis and Management
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Plant Pathogenic Bacteria Studies
- Urinary Tract Infections Management
- Erythrocyte Function and Pathophysiology
- Pneumonia and Respiratory Infections
- Medical and Biological Sciences
- Pharmaceutical studies and practices
- Microplastics and Plastic Pollution
- Viral gastroenteritis research and epidemiology
- Microbial Metabolism and Applications
- Transgenic Plants and Applications
Imperial College London
2022-2025
NIHR Imperial Biomedical Research Centre
2022-2025
University of Surrey
2018-2024
UK Health Security Agency
2024
Hammersmith Hospital
2023
Norwegian Veterinary Institute
2022
Instituto de Biomedicina y Biotecnología de Cantabria
2015-2021
Universidad de Cantabria
2015-2021
Consejo Superior de Investigaciones Científicas
2015-2019
Universidad de La Laguna
1985
The chicken is the most abundant food animal in world. However, despite its importance, gut microbiome remains largely undefined. Here, we exploit culture-independent and culture-dependent approaches to reveal extensive taxonomic diversity within this complex microbial community.
ABSTRACT Bacterial conjugation constitutes a major horizontal gene transfer mechanism for the dissemination of antibiotic resistance genes among human pathogens. Antibiotic spread could be halted or diminished by molecules that interfere with process. In this work, synthetic 2-alkynoic fatty acids were identified as novel class inhibitors. Their chemical properties investigated using prototype 2-hexadecynoic acid and its derivatives. Essential features effective inhibitors carboxylic group,...
Summary Bacterial conjugation is the main mechanism responsible for dissemination of antibiotic resistance genes. Hence, search specific inhibitors paramount in fight against spread these In this pursuit, unsaturated fatty acids have been found to specifically inhibit bacterial conjugation. Despite growing interest on compounds, their mode action and target remain unknown. Here, we identified TrwD, a Type IV secretion traffic ATPase, as molecular acid‐mediated inhibition Moreover, 2‐alkynoic...
Bacterial conjugation is the main mechanism for dissemination of multiple antibiotic resistance in human pathogens. This could be controlled by molecules that interfere with process. A search inhibitors among a collection 1,632 natural compounds, identified tanzawaic acids and B as best hits. They specially inhibited IncW IncFII conjugative systems, including plasmids mobilized them. Plasmids belonging to IncFI, IncI, IncL/M, IncX IncH incompatibility groups were targeted lesser extent,...
Carbapenemase-producing Enterobacterales (CPE) are challenging in healthcare, with resistance to multiple classes of antibiotics. This study describes the emergence IMP-encoding CPE amongst diverse species between 2016 and 2019 across a London regional network.
Plasmid conjugation is a major route for the spread of antibiotic resistance genes. Inhibiting has been proposed as feasible strategy to stop or delay propagation Several compounds have shown be inhibitors in vitro, specifically targeting plasmid horizontal transfer machinery. However, vivo efficiency and applicability these clinical environmental settings remained untested. Here we show that synthetic fatty acid 2-hexadecynoic (2-HDA), when used fish food supplement, lowers frequency model...
Abstract Objective Staphylococcus capitis is part of the human microbiome and an opportunistic pathogen known to cause catheter-associated bacteraemia, prosthetic joint infections, skin wound among others. Detection S. in normally sterile body sites saw increase over last decade England, where a multidrug-resistant clone, NRCS-A, was widely identified blood samples from infants neonatal intensive care units. To address lack complete genomes antibiograms public databases, we performed long-...
Streptococcus agalactiae research primarily centers on investigating human and bovine infections, although this pathogen also can be carried cause infections in a wider range of animal species. Moreover, with S. are posing significant health implications and, also, recent studies highlighting potential zoonotic risk. Despite the comparatively frequent isolation from elephants, only few reports document wild zoo populations. We performed comparative genomic analysis 24 elephant isolates three...
ABSTRACT Klebsiella pneumoniae is an opportunistic human pathogen, particularly associated with nosocomial infections and multidrug resistance. Here, we present a draft genome sequence of carbapenem-resistant K. ST6260 isolated from the catheter tip female patient in referral case received at Kathmandu Model Hospital, Kathmandu, Nepal.
Abstract Improvements in cost and speed of next generation sequencing (NGS) have provided a new pathway for delivering disease diagnosis, molecular typing, detection antimicrobial resistance (AMR). Numerous published methods protocols exist, but lack harmonisation has hampered meaningful comparisons between results produced by different methods/protocols vital global genomic diagnostics surveillance. As an exemplar, this study evaluated the sensitivity specificity five well-established...
The genus Escherichia has been extensively studied and it is known to encompass a range of commensal pathogenic bacteria that primarily inhabit the gastrointestinal tracts warm-blooded vertebrates. However, presence E. coli as model organism potential pathogen diverted attention away from strains other species in genus. To investigate diversity healthy chickens, we collected fecal samples antibiotic-free Lohmann Brown layer hens determined genome sequences 100 isolates, 81 which were...
Vibrio cholerae O1 El Tor is an aquatic Gram-negative bacterium responsible for the current seventh pandemic of diarrheal disease, cholera. A previous whole-genome analysis on V. strains from 2010 epidemic in Pakistan showed that all contained pathogenicity island-1 and accessory colonisation gene acfC (VC_0841). Here we show possess open reading frame 770 bp encoding a protein with predicted size 28 kDa, which shares high amino acid similarity two adhesion proteins found other...
ObjectiveIncreased incidence of neonatal Staphylococcus capitis bacteraemia in summer 2020, London, raised suspicion widespread multidrug-resistant clone NRCS-A. We set out to investigate the molecular epidemiology this units (NNUs) across UK.MethodsWe conducted whole-genome sequencing (WGS) on presumptive S. NRCS-A isolates collected from infants admitted NNUs and environmental sampling two distinct 2021. Previously published genomes were added for comparison. Genetic clusters defined based...
Background Antimicrobial resistance (AMR) is a global threat. Monitoring using an integrated One Health approach essential to detect changes in AMR occurrence. Aim We aimed genes pathogenic and commensal Escherichia coli collected 2013–2020 within monitoring programmes research from food animals, (fresh retail raw meat) humans six European countries, compare vertical horizontal transmission. Methods whole genome sequenced (WGS) 3,745 E. isolates , detected ResFinder performed phylogenetic...
Bacteria display a variety of mechanisms to control plasmid conjugation. Among them, fertility inhibition (FI) systems prevent conjugation co-resident plasmids within donor cells. Analysis the between conjugative could provide new alternatives fight antibiotic resistance dissemination. In this work, broad host range IncW was analyzed in presence set plasmids. Strong FI against R388 were found IncF/MOBF12 as well IncI/MOBP12 plasmids, represented by F and R64, respectively. both cases,...
IncL/M broad-host-range conjugative plasmids are involved in the global spread of blaOXA-48 and emergence blaNDM-1. The aim this study was to evaluate transmission potential encoding emergent NDM-1 carbapenemase compared pandemic OXA-48. conjugation rate fitness cost IncM2 IncL these genes were tested using a variety host bacteria. Genomic analysis uropathogenic Escherichia coli SAP1756 revealed that blaNDM-1 encoded on an plasmid, which also harboured blaTEM-1, bleMBL sul1 highly similar...
Antibiotic persistence is a phenomenon observed when genetically susceptible cells survive long-term exposure to antibiotics. These 'persisters' are an intrinsic component of bacterial populations and stem from phenotypic heterogeneity. Persistence antibiotics concern for public health globally, as it increases treatment duration can contribute failure. Furthermore, there growing array evidence that 'stepping-stone' the development genetic antimicrobial resistance. Urinary tract infections...
Abstract Background: The chicken is the most abundant food animal in world. However, despite its importance, gut microbiome remains largely undefined. Here, we exploit culture-independent and culture-dependent approaches to deliver a genomic blueprint of this complex microbial community. Results: We performed metagenomic sequencing fifty faecal samples from two breeds analysed these, alongside all (n=582) relevant publicly available metagenomes, cluster over 20 million non-redundant genes...
<title>Abstract</title> Objective <italic>Staphylococcus capitis</italic> is part of the human microbiome and an opportunistic pathogen known to cause catheter-associated bacteraemia, prosthetic joint infections, skin wound among others. Detection <italic>S. in normally sterile body sites saw increase over last decade England, where a multidrug-resistant clone, NRCS-A, was widely identified blood samples from infants neonatal intensive care units. To address lack complete genomes...