A5064745227- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Viral Infections and Outbreaks Research
- Infections and bacterial resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral gastroenteritis research and epidemiology
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Vectors
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Biosensors and Analytical Detection
- Bacterial biofilms and quorum sensing
- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- Infective Endocarditis Diagnosis and Management
- Microbial Metabolism and Applications
- Renal Transplantation Outcomes and Treatments
- Toxoplasma gondii Research Studies
UK Health Security Agency
2022-2025
NIHR Imperial Biomedical Research Centre
2025
Imperial College London
2025
Public Health England
2013-2022
University of Alberta
2020
Mayo Clinic
2013
CNR de la Résistance aux Antibiotiques
2010
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2004-2010
Centre Hospitalier de Roubaix
2008
Centre Hospitalier de l’Université de Montréal
2001
To document the intra/interrater reliability and construct validity of Muscular Impairment Rating Scale (MIRS) in assessing patients with myotonic dystrophy type 1 (DM1). The MIRS is a ordinal five-point rating scale, established accordance clinically recognized distal to proximal progression muscular involvement DM1, based partly on manual muscle testing (MMT) 11 groups.To assess MIRS, 55 DM1 were examined by three different observers, one them evaluating each patient twice. Intra-...
The project "Antibiotic resistance in bacteria of animal origin – II" (ARBAO-II) was funded by the European Union (FAIR5-QLK2-2002-01146) for period 2003–2005, with aim to establish a continuous monitoring antimicrobial susceptibility among veterinary laboratories countries based on validated and harmonised methodologies. Available summary data testing bacterial pathogens from different were collected. Antimicrobial several bovine obtained over three year (2002–2004). Each participating...
Abstract Objectives To assess the performance of three commercial molecular assays for detecting major families carbapenemases in pure bacterial isolates. Methods A panel 450 isolates with previously defined carbapenem resistance mechanisms was tested using Check-Direct CPE kit, eazyplex® SuperBug complete kit and Xpert® Carba-R kit. Isolates included 438 Enterobacteriaceae 12 Pseudomonas spp. comprising 100 each KPC, NDM, VIM or OXA-48-like enzymes, two producing both an NDM enzyme, 24 IMP...
We assessed the activity of ceftolozane/tazobactam against consecutive isolates collected in BSAC Bacteraemia Surveillance from 2011 to 2015 and 'problem' sent UK national reference laboratory July 2015, when routine testing began.Susceptibility was by agar dilution with resistance mechanisms identified PCR interpretive reading.Data were reviewed for 6080 surveillance 5473 referred organisms. Ceftolozane/tazobactam had good unselected ESBL producers series, but reduced ertapenem-resistant...
Salmonella genomic island 1 (SGI1) contains an antibiotic resistance gene cluster and has been previously identified in multidrug-resistant enterica serovars Typhimurium DT104, Agona, Paratyphi B. We a variant SGI1 antibiotic-resistance strain of S. serovar Albany isolated from food fish Thailand imported to France. In this strain, the streptomycin aadA2 cassette one integrons was replaced by dfrA1 cassette, conferring trimethoprim open reading frame unknown function. Thus, represents fourth...
OXA-48-like carbapenemases have spread worldwide since 2001. We analysed patient and microbiological data for UK isolates with these enzymes as confirmed by the national reference laboratory from November 2007 to December 2014.MICs were determined using BSAC agar dilution. Isolates reduced susceptibility or resistance at least one carbapenem high-level both piperacillin/tazobactam (MICs ≥64 mg/L) temocillin ≥128 screened PCR bla genes. The genomes of about half sequenced, MLST types, genes...
Gram-negative bacteria with diverse carbapenemases, including New Delhi metallo-β-lactamase (NDM) enzymes, have been increasingly recorded in the UK since 2007. We analysed patient data for NDM-positive isolates confirmed by national reference laboratory from laboratories February 2008 to July 2013.Isolates resistant carbapenems and imipenem MICs reduced ≥8-fold EDTA were tested PCR genes encoding acquired class B carbapenemases. determined BSAC agar dilution methodology. When requested...
Ceftazidime/avibactam combines an established oxyimino-cephalosporin with the first diazabicyclooctane β-lactamase inhibitor to enter clinical use. We reviewed its activity against Gram-negative isolates, predominantly from UK, referred for resistance investigation in year of routine testing, beginning July 2015.Isolates were as received referring laboratories; there is a bias submit those suspected carbapenem resistance. Identification was by MALDI-TOF mass spectroscopy, and susceptibility...
Many isolates of Escherichia coli carrying bla OXA-48 referred to Public Health England's national reference laboratory during 2014 and 2015 shared similar pulsed-field gel electrophoresis (PFGE) profiles, despite coming from patients in multiple different hospitals regions. Whole genome sequencing on an Illumina platform revealed that these belonged sequence type (ST) 38. The gene is usually carried a 62 kb IncL/M plasmid (pOXA48a), but those belonging this ST appeared either lack elements...
Abstract Carbapenem resistance in Gram-negative bacteria is a public health concern. Consequently, numerous government and agency reports discuss carbapenem-resistant Enterobacterales (CRE) organisms (CROs). Unfortunately, these terms are fuzzy. Do they include (1) Proteeae with inherent imipenem resistance; (2) porin-deficient resistant to ertapenem but not other carbapenems; (3) OXA-48–like enzymes that remain “carbapenem susceptible” at breakpoint; (4) Pseudomonas aeruginosa merely lack...
Background Ceftazidime/avibactam, launched in the United Kingdom (UK) March 2017, is an antibiotic against multidrug-resistant Gram-negative pathogens. It was selected for government’s subscription model pilot, incentivising new development, which began December 2020. Aim Ahead of we assessed ceftazidime/avibactam testing, resistance (2016−2020) and usage (2017−2020) levels England, as baselines future surveillance. Methods From routine surveillance samples, retrieved reported...
A total of 1,197 diarrheic children less than 15 years old were investigated for parasitic, bacterial, and viral enteropathogens from March 1981 through February 1982 in the Central African Republic. One or more pathogens identified 49.4% patients. Rotavirus was most frequently pathogen among 18 months old. Enteropathogenic Escherichia coli second isolated (12.1%) 2 age. Campylobacter jejuni also 5 age (10.9%). Entamoeba histolytica very young found to be frequent enteropathogen associated...
To identify the mechanism(s) underlying cefotaxime resistance in 118 of 21 641 (0.55%) non-typhoidal Salmonella enterica isolates collected from humans throughout England and Wales January 2010 to September 2012. Non-duplicate (n = 118) resistant (MICs >1 mg/L) were screened by PCR for genes encoding CTX-M extended-spectrum β-lactamases (ESBLs) associated ISEcp1-like elements, acquired AmpC, SHV, TEM, VEB, PER GES β-lactamases. Sequencing was used specific alleles selected isolates....
To estimate UK prevalence and incidence of clinically significant carbapenemase-producing Enterobacteriaceae (CPE), to determine epidemiological characteristics, laboratory methods infection prevention control (IPC) measures in acute care facilities.A 6 month survey was undertaken November 2013-April 2014 21 sentinel laboratories as part the European Survey on Carbapenemase-Producing (EuSCAPE) project. Up 10 consecutive, non-duplicate, carbapenem-non-susceptible isolates Escherichia coli or...
We evaluated the RAPIDEC(®) CARBA NP assay (bioMérieux SA, Marcy-l'Étoile, France), a colorimetric test for rapid detection of carbapenemases, at two sites: Karolinska University Laboratory and PHE's national reference laboratory.A total 138 bacterial isolates previously characterized as positive class A, B and/or D carbapenemase genes supposed non-carbapenemase producers were tested with according to manufacturer's protocol. Two carbapenemase-producing carried both NDM OXA-48-like genes....
Abstract Background ESBL- and carbapenemase-producing Pseudomonas aeruginosa are prevalent in, for example, the Middle East, Eastern Europe Latin America, though rarer elsewhere. Because P. readily mutate to become carbapenem resistant via loss of OprD, isolates producing ESBLs often as broadly those carbapenemases. We hypothesized that: (i) relebactam might overcome class A carbapenemases directly in aeruginosa; (ii) relebactam’s inhibition AmpC, which gives a generalized potentiation...
To establish the prevalence of mobile colistin resistance (mcr) genes amongst Salmonella enterica isolates obtained through public health surveillance in England (April 2014 to September 2017), 33 205 S. genome sequences from human, food, animal and environmental were screened for presence mcr variants 1 8. The mcr-positive genomes assembled, annotated characterized according plasmid type. Nanopore sequencing was performed on six selected with putative novel plasmids, phylogenetic analysis...