We used cell-free culture filtrate of Pseudomonas aeruginosa as a reducing mediator AgNO3 to silvernanoparticles (AgNPs) and possibly potential anticancer agent against thyroid cancer cells (TPC1). The bio-generation AgNPs was firmly established by taking UV spectrum at 380–500 nm wavelength. Fourier transform analysis reveals the association alcohol, phenol aromatic functional groups with P. synthesized (Ps-AgNPs). By observing under transmission electron microscopy (TEM), size structure...

Context Lappaol F (LAF), a natural lignan from Arctium lappa Linné (Asteraceae), inhibits tumor cell growth in vitro and vivo. The underlying mechanism involves the suppression of Yes-associated protein. However, specific role LAF cycle regulation remains unknown.Objective This study determined molecular by which regulates progression.Materials methods Various colon cancer lines (SW480, HCT15, HCT116) were treated with (25, 50, 75 μmol/L) for 48 h. effects on proliferation using...

AKT1E17K is a gain-of-function mutation that constitutively activates the PI3K-AKT pathway. However, how regulated in cancer pathogenesis remains elusive. Here, RNA immunoprecipitation sequencing (RIP-seq) performed to interrogate AKT1E17K-interacting lncRNAs and identify SVIL-AS1 preferentially binds rather than AKT1WT proteins. It found enhances AKT1 phosphorylation downstream signaling. knockdown dramatically inhibits growth of cells vitro vivo. Notably, interaction...

To dissect and penetrate complexicity regarding the tissue-specific environment-induced expression modes of cytosolic plastidial terpene biosynthetic genes in A. annua, corresponding mRNAs relevant to biosynthesis were quantitatively compared among distinctive organs during different growth stages. Although all examined gradually elevate from June August tested organs, a putative artemisinin responsible DBR2 mRNA represents most abundant transcript anyplace anytime. Apart others, senescent...

Abstract Although it has been reported that arctigenin (ARG) can reduce the body weight and inhibit adipogenic differentiation by activating AMP‐activated protein kinase (AMPK), exact signals responsible for ARG‐mediated antiobesity mechanism through AMPK are not well understood. In this study, we investigated potential improvement of AGR on lipid metabolism using a high‐fat diet (HFD)‐induced hyperlipidemia rats 3T3‐L1 mature adipocytes. The levels its downstream factors were examined...

To investigate the dynamic fluctuation of terpenoid relevant transcriptomics in transgenic Artemisia annua plants that express genomic integrated antisense squalene synthase gene (asSS), we have quantified transcript levels sterol anabolic SS as well artemisinin biogenetic amorphadiene (ADS), cytochrome P450 monooxygenase (CYP71AV1) and reductase (CPR) genes by real-time fluorescent quantitative polymerase chain reaction (RFQ-PCR). The mRNA level sharply droped to 7.4 % – 55.3 (i. e., 44.7...

Background: Garcinia kola is recommended in African folk medicine for the treatment of diabetes and its associated complications. In this study, we investigated effects biflavonoid 1 (GB1), an active chemical component kola, on blood glucose lipid db/db mice with type 2 diabetes. Methods: Db/db were divided into 4 groups: vehicle, low-dose GB1 (50 mg/kg), high-dose (100 rosiglitazone, treated weeks. Body weight, fasting (FBG), insulin, oral tolerance test (OGTT), insulin (ITT), hepatic...

The present study investigated the effects of cajanonic acid A (CAA), extracted from leaves Cajanus cajan (L.) Millsp with a purity 98.22%, on regulatory mechanisms glucose and lipid metabolism. HepG2 cells transfected protein‑tyrosine phosphatase 1B (PTP1B) overexpression plasmid were established. cells, induced insulin resistance by dexamethasone (Dex) treatment, together type 2 diabetes mellitus (T2DM) model rats ob/ob mice, used in study. CAA treatment differentiation 3T3‑L1 adipocytes...

Aryl hydrocarbon receptor (AhR) agonists are potential therapeutic agents for ulcerative colitis (UC). Indirubin (IDR), which is a natural AhR ligand approved leukemia treatment, ameliorates dextran sulfate sodium (DSS)-induced in mice. However, the mechanisms of IDR unknown, limiting its application.This study explores DSS-induced using transcriptomic analysis.Male BALB/c mice were categorized to six groups: normal, DSS model (2% DSS), treatment (10, 20 and 40 mg/kg), sulfasalazine (520...

Lappaol F (LAF), a natural lignan from Arctium lappa Linné (Asteraceae), inhibits tumour cell growth by inducing cycle arrest. However, its underlying anticancer mechanism remains unclear.The effects of LAF on the Hippo-Yes-associated protein (YAP) signalling pathway, which plays an important role in cancer progression, were explored this study.Cervical (HeLa), colorectal (SW480), breast (MDA-MB-231) and prostate (PC3) lines treated with at different concentrations durations. BALB/c nude...

Abstract Rationale: Multiple syphilitic gummas involving both the brain and spinal cord are quite rare. Central nervous system (CNS) commonly misdiagnosed as CNS tumors, clinical suspicion diagnosis of a gumma by physicians vital to avoiding unnecessary surgeries. Our case emphasizes importance routine serologic syphilis tests standard therapy with penicillin in patients mass. Patient concerns: A 22-year-old previously healthy man presented 9-day history progressive right lower limb...

Focusing on the situation of low helium content in natural gas resource China and high cost extraction, OPEX prediction model extraction that based Response Surface Methodology (RSM) is proposed. This method applies ASPEN-HYSYS software to simulate process flow for a given product composition, pressure, temperature; Applying Design Expert module Methodology(RSM) parameter design, combined with existing projects, determine key influencing factors upper lower limits OPEX, obtaining...

The investment estimation of the helium extraction project from natural gas is a crucial step in economically obtaining both domestic and international projects. This article employs Aspen HYSYS to simulate process estimate levels Linde Exxon Mobil integrated processes. We investigate influence feed composition processing capacity on costs product returns. results indicate that higher content correlates with increased equipment total capital cost (CAPEX), significantly more sensitive changes...

Although crosstalk between cytosolic and plastidic terpenoid pathways has been validated in many plant species, we report here for the first time a striking elevation of nucleus-encoded artemisinin biosynthesis relevant DBR2 mRNA following incubation plants with fosmidomycin (FM). FM decreased singlet oxygen (1O2) scavengers such as β-carotene α-tocopherol subsequently invoked 1O2 burst. The treatment fluridone (FD) neither content nor triggered emission. In conclusion, can up-regulate...

Production of artemisinin in genetically modified microorganisms is an attractive option to enable sufficient supply the effective antimalarial agent. Although a sundry precursors are available from engineered bacteria or yeast, no has been manufactured by engineering any microbial platforms due inaccessibility unidentified steps. To this end, it essential consider how convert artemisinin, either biochemically chemically. establish novel procedure production, we incubate mixture Sacchromyces...