To examine the possible involvement of IL-6 in bone metabolism, a mouse osteoblastic cell line (MC3T3-E1) and primary osteoblast-like cells from fetal calvaria were cultured with several systemic local bone-resorbing agents their expression mRNA was determined. Local such as IL-1 alpha, beta, TNF-alpha, LPS greatly induced both MC3T3-E1 cells. Parathyroid hormone slightly increased but not Neither nor 1 alpha,25-dihydroxyvitamin D3 either In agreement mRNA, biologically active produced...

Background— C-reactive protein (CRP) and interleukin (IL)-6 are important risk factors for atherosclerosis coronary heart disease. In the present study, we examined serum levels of CRP IL-6, IL-6 production by monocytes, effect nasal continuous positive airway pressure (nCPAP) in patients with obstructive sleep apnea syndrome (OSAS). Methods Results— After polysomnography, venous blood was collected at 5 am from 30 OSAS 14 obese control subjects. Serum spontaneous monocytes were...

Several lines of evidence suggest that incretin-based therapies suppress the development cardiovascular disease in type 2 diabetes. We investigated possibility glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) can prevent atherosclerosis Apoe −/− mice. mice (17 weeks old) were administered GLP-1(7–36)amide, GLP-1(9–36)amide, GIP(1–42) or GIP(3–42) for 4 weeks. Aortic atherosclerosis, oxidised LDL-induced foam cell formation related gene expression exudate...

Three measures--heart rate, a global index of the influence autonomic nervous system on heart; circulating concentrations adiponectin, an adipose-specific protein; and C-reactive protein (CRP), sensitive marker inflammation--have been reported to be closely associated with insulin resistance. Patients borderline hypertension are known more resistant dyslipidemic than those normal blood pressure (BP). BP can classified into three categories: optimal, normal, high-normal. The present study...

A preponderance of small, dense (sd) LDL is strongly associated with the development coronary heart disease, but method for measurement sd too laborious clinical use. We report a simple quantification that applicable to an autoanalyzer. This consists two steps: first, precipitate lipoprotein density (d) <1.044 g/ml using heparin-magnesium; and second, measure LDL-cholesterol in supernatant by homogenous or apolipoprotein B (apoB) immunoturbidometric assay. The cholesterol apoB values...

Abstract Background The sodium-glucose co-transporter-2 (SGLT-2) inhibitors have been reported to increase both low-density lipoprotein (LDL) and high-density (HDL)-cholesterol (C). This study aimed determine how SGLT-2 affect LDL HDL-C subspecies. Methods single center, open-label, randomized, prospective included 80 patients with type 2 diabetes taking prescribed oral hypoglycemic agents. Patients were allocated receive dapagliflozin (n = 40) or sitagliptin as add-on treatment. Fasting...

Plasma concentrations of small dense (sd)-LDL are associated with the prevalence cardiovascular events. However, special equipment and long assay times required for sd-LDL measurement have hindered its clinical application. Herein, we report development a simple homogeneous sd-LDL-cholesterol (C) adaptable to autoanalyzers.We identified suitable surfactants phospholipases by screening those selective fraction (d 1.044-1.063 kg/L) dissociation other lipoproteins, including large buoyant LDL...

Omentin-1, a novel adipocytokine expressed in visceral fat tissue, is negatively correlated with obesity, insulin resistance, and stable coronary artery disease (CAD). However, there have been no previous reports regarding the effects of omentin-1 on atherogenesis. This study was performed to evaluate atheroprotective human monocyte-derived macrophages, aortic smooth muscle cells (HASMCs) vitro, lesions Apoe−/− mice vivo. The histological expression epicardial adipose tissues its plasma...

Direct associations between hyperglycemia and atherosclerosis remain unclear. We investigated the association amelioration of glycemia by sodium-glucose cotransporter 2 inhibitors (SGLT2is) macrophage-driven in diabetic mice. administered dapagliflozin or ipragliflozin (1.0 mg/kg/day) for 4-weeks to apolipoprotein E-null (Apoe−/−) mice, streptozotocin-induced Apoe−/− db/db then determined aortic atherosclerosis, oxidized low-density lipoprotein (LDL)-induced foam cell formation, related gene...

Adropin, a peptide hormone expressed in liver and brain, is known to improve insulin resistance endothelial dysfunction. Serum levels of adropin are negatively associated with the severity coronary artery disease. However, it remains unknown whether could modulate atherogenesis. We assessed effects on inflammatory molecule expression human THP1 monocyte adhesion umbilical vein cells (HUVECs), foam cell formation monocyte-derived macrophages, migration proliferation aortic smooth muscle...

To determine the association of daily and day-to-day glucose variability with oxidative stress.This was a cross-sectional analysis 68 patients type 2 diabetes mellitus (T2DM) over 72h continuous monitoring. Fasting plasma (FPG) hemoglobin A1c (HbA1c) were measured before breakfast on day 1. Glucose variability, mean level (MGL), amplitude glycemic excursions (MAGE), differences (MODD) in levels area under postprandial curve (AUCPP) days 3. Plasma oxidant capacity against...

Recently, we established a simple method for the quantification of small dense LDL cholesterol (C) using heparin-magnesium precipitation. The LDL-C level was identical to in denser fraction with density 1.044 1.063 g/mL. aim this study examine clinical significance precipitation LDL-C.Small measured by direct homogenous assay supernatant that remained after <1.044 lipoproteins. In 313 normolipidemic subjects, mean value 31+/-13 mg/dL. 462 healthy levels were positively correlated serum...

In the present study, authors examined relationship between lipid peroxidation and inflammation in patients with obstructive sleep apnoea (OSA). A total of 40 obese OSA were studied, along 18 12 lean subjects without OSA. Overnight excretion 8-isoprostane urine serum levels high-sensitivity C-reactive protein (hsCRP) measured. addition, effects 3 months’ treatment nasal continuous positive airway pressure (nCPAP) studied 20 moderate-to-severe urinary hsCRP significantly higher compared mild...

Recent evidence suggests that small dense low-density lipoprotein (sd-LDL) particles are more atherogenic than large-LDL in spite of their lower cholesterol content. This study aimed to determine whether sd-LDL-cholesterol (sd-LDL-C) is superior LDL-C as a biomarker coronary heart disease (CHD).LDL particle size determined by gradient gel electrophoresis and sd-LDL-C concentrations quantified heparin-magnesium precipitation were compared between 482 stable CHD patients 389 non-diabetic...

Although glucose-dependent insulinotropic polypeptide (GIP) levels have been characterized previously, GLP-1 in Asians remain unclear. Here, we investigate total and intact of GLP-1, as well GIP during oral glucose meal tolerance tests (OGTT MTT) Japanese patients with or without type 2 diabetes (T2DM). Seventeen healthy controls 18 age-matched untreated T2DM short duration participated the present study. Fasting GPL-1 were similar between two groups (approximately 15 pM), considerably low...

We recently reported that glucose-dependent insulinotropic polypeptide (GIP) prevents the development of atherosclerosis in apolipoprotein E-null (Apoe(-/-)) mice. GIP receptors (GIPRs) are found to be severely down-regulated diabetic animals. examined whether can exert anti-atherogenic effects diabetes.Nondiabetic Apoe(-/-) mice, streptozotocin-induced and db/db mice were administered (25 nmol/kg/day) or saline (vehicle) through osmotic mini-pumps for 4 weeks. The animals assessed aortic...

Aim: The aim of the present study was to investigate how small dense low-density lipoprotein cholesterol (sdLDL-C) compared with LDL-C affect long-term prognosis in patients stable coronary artery disease (CAD). Methods: sdLDL-C measured by heparin magnesium precipitation and LDL particle size non-denatured gradient-gel electrophoresis were 190 consecutive CAD who underwent arteriography between 2003 2004 did or not develop cardiovascular events during a seven-year follow-up period....

Abstract Catestatin, a catecholamine-release inhibitory peptide, has multiple cardiovascular activities. Conflicting results have been recently reported by increased or decreased plasma levels of catestatin in patients with coronary artery disease (CAD). However, there no previous reports regarding the effects on arteriosclerosis. This study evaluated vasoprotective human macrophages, aortic smooth muscle cells (HASMCs) and umbilical vein endothelial (HUVECs) vitro, atherosclerosis wire...