A5004163453- Gut microbiota and health
- T-cell and B-cell Immunology
- Celiac Disease Research and Management
- Immune Cell Function and Interaction
- Microscopic Colitis
- Diet and metabolism studies
- Gastrointestinal motility and disorders
- Parkinson's Disease Mechanisms and Treatments
- Heat shock proteins research
- Tryptophan and brain disorders
- Alzheimer's disease research and treatments
- Bipolar Disorder and Treatment
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Birth, Development, and Health
- Metabolomics and Mass Spectrometry Studies
- Antimicrobial Resistance in Staphylococcus
- Neurological diseases and metabolism
- Fatty Acid Research and Health
- Immunodeficiency and Autoimmune Disorders
- Biosimilars and Bioanalytical Methods
Stellenbosch University
2023-2025
South African Medical Research Council
2023-2025
Leiden University
1992-1993
De Nederlandsche Bank
1992
Leiden University Medical Center
1991
Schizophrenia (SCZ) is a chronic and severe mental disorder with complex molecular aetiology. Emerging evidence indicates potential association between the gut microbiome development of SCZ. Considering under-representation African populations in SCZ research, this study aimed to explore within South cohort. Gut microbial DNA was obtained from 89 participants (n = 41 cases; n 48 controls) underwent 16S rRNA (V4) sequencing. Data preparation taxa classification were performed DADA2 pipeline R...
Increased numbers of TcR gamma delta + T cells are present in the small intestinal epithelium patients with coeliac disease (CoD). Their function, however, is unknown. In order to facilitate detailed functional studies, have been isolated from biopsies CoD (n = 18) and controls 14). As expected, increased V 1+ were detected freshly intraepithelial cell suspensions (IEL) patients. Also, vitro expanded IEL T-cell populations compared similar cultures control From derived six patients, 107...
Abstract The molecular mechanisms underlying posttraumatic stress disorder (PTSD) are yet to be fully elucidated, especially in underrepresented population groups. Expression quantitative trait loci (eQTLs) DNA sequence variants that influence gene expression, a local ( cis‐ ) or distal trans‐ manner, and subsequently impact cellular, tissue, system physiology. This study aims identify genetic associated with expression changes South African PTSD cohort. Genome‐wide genotype RNA‐sequencing...
Staphylococcal enterotoxins (SE) are known to stimulate a large proportion of T cells. SE bind MHC-class II molecules on APC and particular segment certain TCR V beta gamma gene products. Resting human cells do not express HLA class Ag therefore cannot present Activated cells, however, HLA-DR, -DP, -DQ could consequently serve as for SE. As such, local immune responses might be regulated and/or abrogated by SE-mediated T-T cell interactions leading destruction. We have investigated if such...
Staphylococcal enterotoxins (SEs) are known superantigens for T cells expressing the αβ T‐cell receptor (TCR). They bind to MHC class II molecules on antigen‐presenting and can subsequently trigger responses by binding Vβ‐gene products. The reactivity of γδ with is less well defined although both proliferative cytotoxic have been described. In present study we tested a panel 41 clones against target coated SEA, SEB, SEC1, SEC2, SEC3, SED, SEE or TSST. Three reaction patterns were observed...
<title>Abstract</title> Background Schizophrenia (SCZ) is a chronic and severe mental disorder with complex molecular aetiology. Emerging evidence indicates potential association between the gut microbiome development of SCZ. Considering under-representation African populations in SCZ research, this study aimed to explore within South cohort. Methods Gut microbial DNA was obtained from 89 participants (n = 41 cases; n 48 controls) underwent 16S rRNA (V4) sequencing. Data preparation taxa...
Posttraumatic stress disorder (PTSD) may develop following trauma exposure; however, not all trauma-exposed individuals PTSD, suggesting the presence of susceptibility and resilience factors. The gut microbiome host genome, which are interconnected, have been implicated in aetiology PTSD. However, their interaction has yet to be investigated a South African population. Using genome-wide genotype data 16S rRNA gene V4 sequencing from 53 controls 74 PTSD cases, we observed no significant...
Introduction Studies have reported differences in the gut microbiome of neuropsychiatric disorder cases associated with increased intestinal permeability and inflammatory conditions. In support conditions, cytokine concentration between controls also been Parkinson's disease (PD), schizophrenia (SCZ), posttraumatic stress (PTSD). This study correlated existing data within each cohort including PD, SCZ, PTSD. Methods Utilising 16S rRNA (V4) microbial available for PTSD, associations...
Depts. of Immunohaematology, Gastroenterology, Pediatrics and Pathology, Academic Hospital, Leiden, The Netherlands.