Bipolar spindle formation is pivotal for accurate segregation of mitotic chromosomes during cell division. A growing body evidence suggests that, in addition to centrosome- and chromatin-based microtubule (MT) nucleation, MT-based MT nucleation plays an important role proper bipolar various eukaryotic organisms. Although a recently discovered Augmin complex appears play central this event, how regulated remains unknown. Here we provide that mammalian polo-like kinase 1 (Plk1) localizes...

Abstract Objective We evaluated the diagnostic performance of CA 125, HE 4, and ROMA for ovarian cancer in Koreans set optimal cutoffs. Method Serum levels 4 125 score were determined 762 patients with benign gynecological disease 70 cancer. Receiver operating characteristic curves constructed to calculate areas under curve ( AUC ). exhibiting maximum Youden index determined, respectively, as cutoffs, sensitivity specificity by applying those Results In diseases, significantly increased...

Approximately 50%-60% of secondary resistance to primary EGFR- tyrosine kinase inhibitors (TKI) therapy is caused by acquired p.Thr790Met (T790M) mutation; however, highly fragmented, low-quantity circulating tumor DNA an obstacle for detecting mutations. Therefore, more sensitive mutation detection techniques are required. Here, we report a new mutant enrichment technology, the CRISPR system combined with post-polymerase chain reaction (PCR) cell-free (cfDNA) (CRISPR-CPPC) detect T790M...

Exosomal nucleic acid (exoNA) is a feasible target to improve the sensitivity of EGFR mutation testing in non-small cell lung cancer patients with limited cell-free DNA (cfDNA) mutant copies. However, type and size exoNA related has not been explored extensively.The was evaluated using ddPCR. A total 47 plasma samples tested short-length exoTNA (exosomal RNA) cfDNA.The (76.5%) higher than that cfDNA (64.7%) for detecting mutations NSCLC patients. In EGFR-mutant intrathoracic disease (M0/M1a)...

Background: Molecular cancer profiling may lead to appropriate trials for molecularly targeted therapies.Cell-free DNA (cfDNA) is a promising diagnostic and/or prognostic biomarker in gastric (GC).We characterized somatic genomic alterations cfDNA of patients with GC.Methods: Medical records and data 81 diagnosed as having GC were reviewed.Forty-nine 32 tested using the Oncomine Pan-Cancer Cell-Free Assay on Ion Torrent platform AlphaLiquid 100 kit Illumina platform, respectively.Results:...

Advances in patient care and immunosuppressive drugs have improved graft survival, resulting an increase kidney transplantation (KT); however, persistent immunosuppression is thought to cause late occurrence of cancer. This population-based study consisted a total 14,842 patients whose data from the years 2002 2017 were collected National Health Information Database South Korea. Malignancies occurred 7.6% KT patients. Prostate thyroid cancers most common males females, respectively. From...

Abstract Apart from the incidence and mortality caused by it, Coronavirus disease (COVID-19) has had a significant impact on other diseases. This study aimed to estimate influences of COVID-19 pandemic tuberculosis (TB) number TB-associated deaths in Republic Korea. A dynamic compartment model incorporating age-structure was developed for studying TB transmission progression using Korean population data. After calibration with notification data South Korea, burden over 6 years (2020–2025)...

Abstract The transcription factor nuclear factor-κB (NF-κB) is activated in response to various stimuli including ionizing radiation. Disruption of NF-κB activation by mutant forms the inhibitor IκB-α or proteasome inhibitors enhances both sensitivity radiation and radiation-induced apoptosis. Human squamous carcinoma SCC-35 cells stably expressing a fragment (residues 1 84) human p65 have been shown exhibit down-regulation endogenous mRNA its protein. protein also inhibited preventing...

Venoms from venomous arthropods, including bees, typically induce an immediate local inflammatory response; however, how venoms acutely elicit response and which components remain unknown. Moreover, the presence of superoxide dismutase (SOD3) in venom its functional link to acute has not been determined date. Here, we confirmed that SOD3 bee (bvSOD3) acts as inducer H2O2 production promote responses. In mouse models, exogenous bvSOD3 rapidly induced overproduction through superoxides are...

Background: Intestinal protozoan infection is one of the main causes gastrointestinal diseases.Protozoa are usually detected by direct smear microscopy, concentration techniques, or special stains; however, these techniques labor-intensive and require well-trained technicians.Therefore, molecular involving polymerase chain reaction (PCR) have been developed to satisfy need for unbiased rapid analytical methods with high sensitivity specificity.In this study, BD MAX TM Enteric Parasite Panel...

Marfan syndrome (MFS) is caused by fibrillin-1 gene (

Detection of EGFR-SEPT14 fusion in cell-free DNA a patient with advanced gastric cancer: A case report

Appropriate reference intervals of serum insulin-like growth factor I (IGF-I) is important for diagnosing and monitoring patients with hormone-related diseases. To establish intervals, adult individuals (<i>n</i>=1,334, 680 men 654 women) were divided into six age groups (20–29, 30–39, 40–49, 50–59, 60–69, ≥70). Serum IGF-I was measured by chemiluminescence immunoassay (Liaison). Concordance patient classification based on manufacturer’s standard deviation score (SDS) evaluated....

Following success of the phase III PROfound trial, poly (ADP-ribose) polymerase (PARP) inhibitor olaparib was approved by US Food and Drug Administration in May 2020 for adult patients with deleterious homologous recombination repair (HRR) gene-mutated metastatic castration-resistant prostate cancer (mCRPC). As locally adopted multigene panel next-generation sequencing (NGS) assays selecting PARP candidates have not been thoroughly evaluated, we compared analytical performance FoundationOne...

오늘날, 가상현실의 대표적 구현장치인 HMD를 활발하게 활용하는 분야는 게임이다. HMD기반 가상현실 게임의 사용자 인터페이스에 메타포를 활용한 디자인이 빈번하게 활용되고 있다. 본 연구의 목적은 새로운 매체인 HMD디바이스의 FPS장르 게임 인터페이스에서 나타나는 메타포 유형을 도출함으로써 해당 인터페이스가 적절히 활용하여 사용자에게 올바른 심성모형을 생성하고 있는지 사례분석을 통해 알아보는데 연구 결과 다수 공통적으로 유형은 정보 지각 측면에서 조작, 예측, 친숙화, 물리적 세계 메타포로 나타났으며 조작 행동 측면에서는 예측성, 친숙화 나타났다. 이는 사용자의 일반적 경험과 지식을 통한 심성모형 구축이 가능하다고 판단된다. 그 외 실수를 방지하기 위한 안정성 메타포, 공간의 정체성을 나타내는 제시 메타포의 사용이 앞으로 필요할 것으로 보인다.

Abstract Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) have significantly improved on clinical outcomes in many non-small cell lung cancer (NSCLC) patients. However, therapeutic efficacy of EGFR TKIs is limited patients who gefitinib-sensitizing gene mutations (Exon 19del, L858R). Although, not as sensitive these mutations, some wild type responded to TKIs. Therefore, we hypothesized that there are underlying mechanisms cause most NSCLC be refractory. In this study,...

and guidelines for the interpretation of sequence variants [1].Criteria classifying pathogenic or benign have been developed.However, some evidence, such as PS3/BS3, is somewhat ambiguous, many laboratories dif culty applying criteria in variant interpretation.According to ACMG/AMP guideline, PS3 BS3 are de ned by "well-established vitro vivo functional studies supportive a damaging effect on gene product" show no protein function splicing," respectively [1].Although can provide evidence...

This article examines the invocation of international responsibility arising from activities Artificial Intelligence(AI). The AI has capabilities to recognize circumstances and determine beyond instruction or control humans, as a result it become important how regulate involving activities. question whether is possible appropriate apply Draft Articles on Responsibility States for Internationally Wrongful Acts adopted by International Law Commission wrongful acts through AI.
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A dynamic clustering method can reconstruct IoT nodes into logical group units when using several groups of nodes. However, massive control at a level does not guarantee optimal performance. To efficiently deliver and execute command within certain communication delay, it is necessary to cluster each subgroups commands by subgroup. This paper proposes the DBDC(Delay-Based Dynamic Clustering) method, based on DBSCAN that optimize delay group. Through simulations, parameters algorithm create...