A5005767836- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Neurological disorders and treatments
- Cellular transport and secretion
- Nerve injury and regeneration
- Innovative Microfluidic and Catalytic Techniques Innovation
- Neuroscience and Neuropharmacology Research
- Inhalation and Respiratory Drug Delivery
- Gut microbiota and health
- Genetic Neurodegenerative Diseases
- Lysosomal Storage Disorders Research
- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Cancer Cells and Metastasis
Jackson Laboratory
2023-2025
University of Pennsylvania
2020-2024
Institute on Aging
2023-2024
UConn Health
2023
Abstract Amyloid-forming proteins such α-synuclein and tau, which are implicated in Alzheimer’s Parkinson’s disease, can form different fibril structures or strains with distinct toxic properties, seeding activities pathology. Understanding the determinants contributing to formation of amyloid features could open new avenues for developing disease-specific diagnostics therapies. Here we report that O-GlcNAc modification monomers results core structure, as revealed by cryogenic electron...
The microbiome regulates the respiratory epithelium's immunomodulatory functions. To explore how microbiome's biodiversity affects microbe-epithelial interactions, we screened 58 phylogenetically diverse microbes for their transcriptomic effect on human primary bronchial air–liquid interface (ALI) cell cultures. We found distinct species- and strain-level differences in host innate immunity epithelial barrier response. Strikingly, that interferon, an antiviral response, was one of most...
Abstract We have developed a high-throughput drug discovery platform, measuring fluorescence resonance energy transfer (FRET) with fluorescent alpha-synuclein (αSN) biosensors, to detect spontaneous pre-fibrillar oligomers in living cells. Our two αSN FRET biosensors provide complementary insight into oligomerization and conformation order improve the success of campaigns for treatment Parkinson’s disease. measure by lifetime, rather than traditional intensity, providing structural readout...
Progenitor human nasal epithelial cells (hNECs) are an essential cell source for the reconstruction of respiratory pseudostratified columnar epithelium composed multiple types in context infection studies and disease modeling. Hitherto, manual seeding has been dominant method creating tissue models through biofabrication. However, this approach limitations terms achieving intricate three-dimensional (3D) structure natural epithelium. 3D bioprinting utilized to reconstruct various models,...
ABSTRACT The process of amyloid fibril formation remains one the primary targets for developing diagnostics and treatments several neurodegenerative diseases (NDDs). Amyloid-forming proteins such α-Synuclein Tau, which are implicated in pathogenesis Alzheimer’s Parkinson’s disease, can form different types structure, or strains, that exhibit distinct structures, toxic properties, seeding activities, pathology spreading patterns brain. Therefore, understanding molecular structural...
Abstract α-Synuclein (aSyn) participates in synaptic vesicle trafficking and transmission but its misfolding is also strongly implicated Parkinson’s disease (PD) other neurodegenerative synucleinopathies which misfolded aSyn accumulates different regions of the central peripheral nervous systems. Although increased expression levels or altered aggregation propensities likely underlie familial PD with SNCA amplification mutations, majority arise sporadically, indicating that can develop under...
ABSTRACT Preventing or reversing the pathological misfolding and self-association of alpha-synuclein (aSyn) can rescue a broad spectrum cellular insults that manifest in Parkinson’s Disease (PD), Dementia with Lewy bodies (DLB), other alpha-synucleinopathies. We have developed high-throughput, FRET-based drug discovery platform combines high-resolution protein structural detection living cells an array functional biophysical assays to identify novel lead compounds protect SH-SY5Y from aSyn...
Abstract Alpha-synuclein (aSyn) participates in synaptic vesicle trafficking and transmission, but its misfolding is also strongly implicated Parkinson’s disease (PD) other neurodegenerative disorders known as synucleinopathies where misfolded aSyn accumulates different regions of the central peripheral nervous systems. Although increased expression levels or altered aggregation propensities likely underlie familial PD with SNCA amplification mutations, majority arise sporadically,...
Human nasal epithelial cells (hNECs) are an essential cell source for the reconstruction of respiratory pseudostratified columnar epithelium composed multiple types in context infection studies and disease modeling. Hitherto, manual seeding has been dominant method creating tissue models. However, approach is slow, low-throughput limitations terms achieving intricate 3D structure natural a uniform manner. Bioprinting utilized to reconstruct various models, such as cutaneous, intestinal,...
Abstract The microbiome modulates the respiratory epithelium’s immunomodulatory functions. To explore how microbiome’s biodiversity affects microbe-epithelial interactions, we screened 58 phylogenetically diverse microbes for their transcriptomic effect on tracheobronchial air-liquid interface cell (ALI) cultures. We found distinct species- and strain-level differences in host innate immunity, epithelial barrier response. Strikingly, that interferon, an antiviral immune response, was one of...