A5102005425- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- RNA modifications and cancer
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- Bacterial Genetics and Biotechnology
- Lymphoma Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- Plant Genetic and Mutation Studies
- Pluripotent Stem Cells Research
- Nematode management and characterization studies
- Plant Gene Expression Analysis
- PI3K/AKT/mTOR signaling in cancer
- Thyroid Disorders and Treatments
- Gene expression and cancer classification
Hormel (United States)
2020-2025
University of Minnesota
2020-2025
Mayo Clinic in Arizona
2024
City University of Hong Kong
2024
Shenzhen Institutes of Advanced Technology
2024
Chinese Academy of Sciences
2024
South China Agricultural University
2024
University of Macau
2024
University of Minnesota System
2024
Mayo Clinic
2014-2023
Recent studies have identified a Lys 27-to-methionine (K27M) mutation at one allele of H3F3A , the two genes encoding histone H3 variant H3.3, in 60% high-grade pediatric glioma cases. The median survival this group patients after diagnosis is ∼1 yr. Here we show that levels H3K27 di- and trimethylation (H3K27me2 H3K27me3) are reduced globally H3.3K27M patient samples due to expression mutant allele. Remarkably, also observed H3K27me3 Ezh2 (the catalytic subunit methyltransferase) chromatin...
How parental histone (H3-H4)2 tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands DNA replication forks for inheritance remains elusive. Here we show that tetramers assembled into nucleosomes both strands, with a slight preference strands. The lagging-strand increases markedly in budding yeast cells lacking Dpb3 Dpb4, two subunits strand polymerase, Pol ε, owing to impairment transfer Dpb3-Dpb4 binds H3-H4 vitro participates...
Faithful inheritance of parental histones is essential to maintain epigenetic information and cellular identity during cell division. Parental are evenly deposited onto the replicating DNA sister chromatids in a process dependent on MCM2 subunit helicase. However, impact aberrant histone partition human disease such as cancer largely unknown. In this study, we construct model impaired by introducing MCM2-2A mutation (defective binding) MCF-7 breast cells. The resulting reprograms...
Chromatin replication is intricately intertwined with the recycling of parental histones to newly duplicated DNA strands for faithful genetic and epigenetic inheritance. The transfer occurs through two distinct pathways: leading strand deposition, mediated by polymerase ε subunits Dpb3/Dpb4, lagging facilitated MCM helicase subunit Mcm2. However, mechanism facilitation Mcm2 transferring while moving along remains unclear. Here, we show that deletion Pol32, a nonessential major lagging-strand...
Abstract Recycling of parental histones is an important step in epigenetic inheritance. During DNA replication, polymerase epsilon subunit DPB3/DPB4 and replication helicase MCM2 are involved the transfer to leading lagging strands, respectively. Single Dpb3 deletion (dpb3Δ) or Mcm2 mutation (mcm2-3A), which each disrupts one histone pathway, leads other's predominance. However, biological impact two pathways on chromatin structure repair remains elusive. In this study, we used budding yeast...
Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcriptional factors that regulate growth, differentiation, and development. The molecular mechanisms by which TRs mediate these effects unclear. One prevailing hypothesis suggests may cooperate with other to their biological effects. In this study, we tested examining whether the activity of is modulated tumor suppressor p53. p53 a protein regulates gene expression via sequence-specific DNA binding and/or direct protein-protein...
Barbara McClintock reported that the Ac/Ds transposable element system can generate major chromosomal rearrangements (MCRs), but underlying mechanism has not been determined. Here, we identified a series of chromosome derived from maize lines containing pairs closely linked Ac termini. Molecular and cytogenetic analyses showed MCRs in these comprised 17 reciprocal translocations two large inversions. The breakpoints all 19 are delineated by termini characteristic 8-base-pair target site...
Alternative transposition can induce genome rearrangements, including deletions, inverted duplications, inversions, and translocations. To investigate the types frequency of rearrangements elicited by a pair reversed Ac/Ds termini, we isolated analyzed 100 new mutant alleles derived from two parental that both contain an intact Ac fractured (fAc) structure at maize p1 locus. Mutants were characterized PCR sequencing; results show nearly 90% (89/100) represent structural translocations, or...
During DNA replication, parental H3-H4 marked by H3K4me3 are transferred almost equally onto leading and lagging strands of replication forks. Mutations in replicative helicase subunit, Mcm2 (Mcm2-3A), strand polymerase Dpb3 (dpb3∆), result asymmetric distributions at replicating immediately following replication. Here, we show that mcm2-3A dpb3∆ mutant cells markedly reduce the distribution during cell cycle progression before mitosis. Furthermore, restoration a more symmetric these is...
Abstract A key question in developmental biology addresses the mechanism of asymmetric cell division. Asymmetry is crucial for generating cellular diversity required development multicellular organisms. As one potential mechanisms, chromosomally borne epigenetic difference between sister cells that changes mating/cell type has been demonstrated only Schizosaccharomyces pombe fission yeast. For technical reasons, it nearly impossible to determine existence such a operating during embryonic...
Composite or closely linked maize (Zea mays) Ac/Ds transposable elements can induce chromosome breakage, but the precise configurations of that lead to breakage are not completely defined. Here, we determined structures and properties 15 p1 alleles: each allele contains a fixed fractured Ac (fAc) element full-length at various flanking sites. Our results show pairs Ac/fAc in which termini different direct reverse orientation breakage. By contrast, no is observed with alleles containing...
The maize Activator (Ac)/Dissociation (Ds) transposable element system has been used in a variety of plants for insertional mutagenesis. Ac/Ds elements can also generate genome rearrangements via alternative transposition reactions which involve the termini closely linked transposons. Here, we introduced transgene containing reverse-oriented together with an Ac transposase gene into rice (Oryza sativa ssp. japonica cv. Nipponbare). Among transgenic progeny, identified and characterized 25...
Schizosaccharomyces pombe, the fission yeast, cells alternate between P- and M-mating type, controlled by alleles of mating-type locus (mat1). The mat1 switching occurs replacing with a copy derived from silenced "donor locus," mat2P or mat3M. mechanism donor choice ensuring that primarily productively to opposite called directionality, is largely unknown. Here we identified mat1-Mc gene, mammalian sex-determination gene (SRY) homolog, as primary dictates directionality in M cells. A...
Significance During the S phase of cell cycle, DNA replication machinery accurately duplicates genome in spite numerous hurdles that cause stress. The checkpoint pathway deals with stress, which, if left undealt with, can lead to fork collapse and instability, a process remains poorly understood. Here, we show couples leading- lagging-strand synthesis prevent generation deleterious single-stranded under stress by attenuating function Mrc1, protein involved both checkpoint, providing...
Abstract The maize Ac/Ds transposon family was the first transposable element system identified and characterized by Barbara McClintock. transposons belong to hAT of class II DNA transposons. We others have shown that elements can undergo a process alternative transposition in which transposase acts on termini two separate, nearby Because these are present different elements, generate variety genome alterations such as inversions, duplications, deletions, translocations. Moreover, transpose...
The clinical manifestations of patients with resistance to thyroid hormone result from inhibition the functions wild-type receptors (wTRs) by dominant negative effect mutant TR beta 1 (mTR 1). One proposed mechanisms which mTR exerts its action is via formation putative inactive wTR 1/mTR heterodimer. However, nature heterodimer poorly understood. present study characterizes electrophoretic mobility shift assay. used was PV, contains a frame mutation in C-terminal part and has less than 1%...