Increasing worldwide demand for food, feed and fuel presents a challenge in light of limited resources climatic challenges. Breeding stress tolerance drought tolerance, particular, is one the most challenging tasks facing breeders. The comparative screening immense numbers plant gene candidates their interactions with environment represents major bottleneck this process. We suggest four key components to be considered pre-field screens (phenotyping) complex traits under conditions: (i)...

Abscisic acid (ABA) levels increase significantly in plants under stress conditions, and ABA is thought to serve as a key stress-response regulator. However, the direct effect of on photosynthesis mesophyll yield both well-watered drought conditions are still subject debate. Here, we examined this issue using transgenic Arabidopsis (

Summary Despite decades of extensive study, the role cuticular lipids in sustaining plant fitness is far from being understood. We utilized genome‐edited tree tobacco ( Nicotiana glauca ) to investigate significance different classes epicuticular wax abiotic stress such as water loss, drought, and light response. generated mutants displaying a range compositions. Four one cutin mutant were extensively investigated for alterations their response factors. Although mutations led elevated did...

The WUSCHEL homeobox transcription factor is required to specify stem-cell identity at the shoot apical meristem and its ectopic expression sufficient induce de novo formation. Yet, manner by which WUS promotes fate not yet fully understood. In present research we address this question inducing function outside of domain. We show that activation in root inhibits responses exogenous auxin suppresses initiation growth lateral roots. Using time lapse movies follow cell-cycle marker...

Abstract Despite decades of research resulting in a comprehensive understanding epicuticular wax metabolism, the function these almost ubiquitous metabolites plant–herbivore interactions remains unresolved. In this study, we examined effects CRISPR‐induced knockout mutations four Nicotiana glauca (tree tobacco) metabolism genes. These cause wide range changes composition, leading to altered with insects and snails. Three interaction classes were examined: chewing herbivory by seven...

ABSTRACT Plants produce an immense diversity of defensive specialized metabolites. However, despite extensive functional characterization, the relative importance different compounds is rarely examined in natural settings. Here, we compare efficacy three Nicotiana benthamiana compounds, nicotine, acylsugars and a serine protease inhibitor, by growing plants with combinations knockout mutations setting, quantifying invertebrate interactions comparing plant performance. Among tested had...

Summary Plants produce an immense diversity of defensive specialized metabolites. However, despite extensive functional characterization, the relative importance different compounds is rarely examined in natural settings. Here, we compare efficacy three Nicotiana benthamiana compounds, nicotine, acylsugars, and a serine protease inhibitor, by growing plants with combinations knockout mutations setting, quantifying invertebrate interactions, comparing plant performance. Among tested...

Abstract An enormous diversity of specialized metabolites is produced in the plant kingdom, with each individual synthesizing thousands these compounds. Previous research showed that benzoxazinoids, most abundant class maize, also function as signaling molecules by regulating production callose a defense response. In this study, we identified catechol acetylglucose (CAG) benzoxazinoid-regulated metabolite from salicylic acid via and catechol-glucoside. Genome wide association studies CAG...

An enormous diversity of specialized metabolites is produced in the plant kingdom, with each individual synthesizing thousands these compounds. Previous research showed that benzoxazinoids, most abundant class maize, also function as signaling molecules by regulating production callose a defense response. We searched for additional benzoxazinoid-regulated metabolites, characterized them, examined whether they too herbivore protection, and determined how Spodoptera frugiperda (fall armyworm),...

In a recent paper in Nature, Edith Heard from the European Molecular Biology Laboratory (EMBL) suggested that molecular biologists should 'reconnect with nature' by diversifying sampling locations. Although this approach has its own benefits, we suggest advanced methods rather be used to take hypothesis-based experiments nature, thereby supplying much-needed context for experimentation under controlled conditions. Following CRISPR (clustered regularly interspaced short palindromic repeats)...

Abstract Abscisic acid (ABA) is known to be involved in stomatal closure. However, its role response rapid increases the vapor pressure deficit (VPD) unclear. To study this issue, we generated guard cell (GC)-specific ABA-insensitive Arabidopsis plants (GC-specific abi1-1 ; GCabi). Under normal conditions, conductance (g s ) and apertures of GCabi were greater than those control plants. This supports GC ABA as limiting maximal aperture under non-stressful conditions. When there was a...

Abstract Despite decades of extensive study, the role cuticular lipids in sustaining plant fitness is far from being understood. To answer this fundamental question, we employed genome editing tree tobacco ( Nicotiana glauca ) plants and generated mutations 16 different lipids-related genes. We chose due to abundant, yet simply composed epicuticular waxes deposited on its surface. Five out 9 mutants that displayed a phenotype were selected for depth analysis. They had either reduced total...

Abstract Despite decades of research resulting in a comprehensive understanding epicuticular wax biosynthesis and metabolism, the function these almost ubiquitous metabolites plant-herbivore interactions remains unresolved. To develop better this role, we investigated four Nicotiana glauca (tree tobacco) genome edited mutants. This included [ eceriferum1 ( cer1) , eceriferum3 cer3), β-ketoacyl-coA synthase6 kcs6 ), fatty acyl-coA reductase far )] displaying wide range alkane alcohol...