A5069474358- Silicon Effects in Agriculture
- Biofuel production and bioconversion
- Aluminum toxicity and tolerance in plants and animals
- Geochemistry and Elemental Analysis
- Advanced Cellulose Research Studies
- Enzyme-mediated dye degradation
- Lignin and Wood Chemistry
- Parkinson's Disease Mechanisms and Treatments
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Interconnection Networks and Systems
- Glycosylation and Glycoproteins Research
Pennsylvania State University
2023-2024
Hebrew University of Jerusalem
2020-2022
Abstract Background Cellulose degradation by cellulases has been studied for decades due to the potential of using lignocellulosic biomass as a sustainable source bioethanol. In plant cell walls, cellulose is bonded together and strengthened polyphenolic polymer, lignin. Because lignin tightly linked not digestible cellulases, thought play dominant role in limiting efficient enzymatic biomass. Removal via pretreatments currently limits cost-efficient production ethanol from cellulose,...
Abstract Silicon dioxide in the form of hydrated silica is a component plant tissues that can constitute several percent by dry weight certain taxa. Nonetheless, mechanism formation mostly unknown. (Si) taken up from soil roots monosilicic acid molecules. The silicic carried xylem and subsequently polymerizes target sites to silica. In sorghum (Sorghum bicolor), aggregates an orderly pattern along inner tangential cell walls endodermis cells. Using Raman microspectroscopy, autofluorescence,...
Degrading cellulose is a key step in the processing of lignocellulosic biomass into bioethanol. Cellobiose, disaccharide product degradation, has been shown to inhibit cellulase activity, but mechanisms underlying inhibition are not clear. We combined single-molecule imaging and biochemical investigations with goal revealing mechanism by which cellobiose inhibits activity Trichoderma reesei Cel7A, well-characterized exo-cellulase. find that slows processive velocity Cel7A shortens distance...
Silica aggregates at the endodermis of sorghum roots. Aggregation follows a spotted pattern locally deposited lignin inner tangential cell walls. Autofluorescence microscopy suggests that non-silicified (-Si) spots are composed two distinct concentric regions varied composition. To highlight variations in chemistry, we used Raman microspectroscopy to map endodermal wall and silica aggregation sites roots grown hydroponically with or without Si amendment. In +Si samples, aggregate center was...
Abstract Hydrated silica (SiO2·nH2O) aggregates in the root endodermis of grasses. Application soluble silicates (Si) to roots is associated with variations balance reactive oxygen species (ROS), increased tolerance a broad range stresses affecting ROS concentrations, and early lignin deposition. In sorghum (Sorghum bicolor L.), aggregation patterned an active silicification zone (ASZ) by special type aromatic material forming spotted pattern. The deposition has signature typical lignin....
Efficient cellulose degradation by cellulase enzymes is crucial for using lignocellulosic biomass in bioenergy production. Single-molecule microscopy showed that xylan hinders the efficiency of inhibiting its binding to and impeding processivity bound enzyme molecules.
Abstract Efficient cellulose degradation by cellulase enzymes is crucial for using lignocellulosic biomass in bioenergy production. In the cell wall of plants, bound lignin and hemicellulose, which are key factors contributing to recalcitrance plant biomass. These non-cellulosic components known interfere with function cellulolytic enzymes. While effects have been studied extensively, contribution xylan, major hemicellulose secondary walls often overlooked. To study those effects, we...
Abstract Degrading cellulose is a key step in the processing of lignocellulosic biomass into bioethanol. Cellobiose, disaccharide product degradation, has been shown to inhibit cellulase activity, but mechanisms underlying inhibition are not clear. We combined single-molecule imaging and biochemical investigations with goal revealing mechanism by which cellobiose inhibits activity Trichoderma reesei Cel7A, well-characterized exo-cellulase. find that slows processive velocity Cel7A shortens...
<p>Silicon oxides are the most abundant mineral group in soils. Therefore, plant roots always exposed to some silicic acid (Si(OH)<sub>4</sub>), which is soluble form of silicates. Monosilicic molecules taken up by roots, carried xylem, and subsequently polymerize silica varied silicifying target sites. This biogenic...
Abstract Background Cellulose degradation by cellulases has been studied for decades due to the potential of using lignocellulosic biomass as a sustainable source bioethanol. In plant cell walls, cellulose is bonded together and strengthened polyphenolic polymer, lignin. Because lignin tightly linked not digestible cellulases, thought play dominant role in limiting efficient enzymatic biomass. Removal via pretreatments currently limits cost-efficient production ethanol from cellulose,...
Abstract Hydrated silica (SiO 2 ·nH O) aggregates in the root endodermis of grasses. Application soluble silicates (Si) to roots is associated with variations balance reactive oxygen species (ROS), increased tolerance a broad range stresses affecting ROS levels, and early lignin deposition. In sorghum ( Sorghum bicolor L.), aggregation patterned an active silicification zone (ASZ) by special type lignin. Since polymerization mediated ROS, we studied formation under varied conditions...