Biochemical analysis of the kinetics assembly two cytoplasmic plaque proteins desmosome, desmoplakins I (250,000 Mr) and II (215,000 Mr), in Madin-Darby canine kidney (MDCK) epithelial cells, demonstrated that these exist a soluble insoluble pool, as defined by their extract ability Triton X-100 high salt buffer (CSK buffer). Upon cell-cell contact, there is rapid increase capacity pool at expense pool; subsequently, stabilized, while remaining continue to be degraded rapidly (Pasdar, M., W....

Desmosomes are composed of two morphologically and biochemically distinct domains, a cytoplasmic plaque membrane core. We have initiated study the synthesis assembly these domains in Madin-Darby canine kidney (MDCK) epithelial cells to understand mechanisms involved formation desmosomes. Previously, we reported kinetics components domain, Desmoplakin I/II (Pasdar, M., W. J. Nelson. 1988. Cell Biol. 106:677-685 106:687-699. now extended this analysis include major glycoprotein component core...

The functional interaction of cells in the formation tissues requires establishment and maintenance cell-cell contact by junctional complex. However, little is known biochemically about mechanism(s) that regulates complex assembly. To address this problem, we have initiated a study regulation assembly one component complex, desmosome, during induction cultures Madin-Darby canine kidney epithelial cells. Here analyzed two major protein components desmosomal plaque, desmoplakins I (Mr 250,000)...

Plakoglobin (γ-catenin) is a member of the Armadillo family proteins and homolog β-catenin. As component both adherens junctions desmosomes, plakoglobin plays pivotal role in regulation cell-cell adhesion. Furthermore, similar to β-catenin, capable participating cell signaling. However, unlike β-catenin that has well-documented oncogenic potential through its involvement Wnt signaling pathway, generally acts as tumor/metastasis suppressor. The exact roles during tumorigenesis metastasis are...

Plakoglobin is a cytoplasmic protein and homologue of beta-catenin Armadillo Drosophila with similar adhesive signaling functions. These proteins interact cadherins to mediate cell-cell adhesion associate transcription factors induce changes in the expression genes involved cell fate determination proliferation. Unlike relatively well characterized role proliferation via activation c-MYC cyclin D1 gene expression, function plakoglobin regulation growth undefined. Here, we show that high...

Desmosomes are major components of the intercellular junctional complex in epithelia. They consist at least eight different cytoplasmic and integral membrane proteins that organized into two biochemically structurally distinct domains: plaque core. We showed previously MDCK epithelial cells (desmoplakin I II; DPI/II) core domains (desmoglein I; DGI) initially enter a pool is soluble buffers containing Triton X-100, then titrate an insoluble before their arrival plasma (Pasdar, M., W. J....

Summary Endothelin receptors B (Ednrb) are involved in the development of enteric and melanocytic lineages, which originate from neural crest cells (NCCs). In mice, trunk NCCs their derivatives express only one Ednrb. quail, two Ednrb: Ednrb Ednrb2. Quail is expressed migrating along ventral pathway, gives rise to peripheral nervous system, including ganglia. Ednrb2 upregulated before these enter dorsolateral pathway. The pathway melanocyte precursors. We analyzed vitro differentiation ovo...

Plakoglobin (γ-catenin), a constituent of the adherens junction and desmosomes, has signaling capabilities typically associated with tumor/metastasis suppression through mechanisms that remain undefined. To determine role plakoglobin during tumorigenesis metastasis, we expressed in human tongue squamous cell carcinoma (SCC9) cells compared mRNA profiles parental SCC9 their plakoglobin-expressing transfectants (SCC9-PG). We detected several p53-target genes whose levels were altered upon...

// Tatiana M. Tilli 1 , Nicolas Carels Jack A. Tuszynski 2, 3 Manijeh Pasdar 2 Laboratory of Biological System Modeling, National Institute for Science and Technology on Innovation in Neglected Diseases (INCT/IDN), Center Technological Development Health (CDTS), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil Department Oncology, Faculty Medicine & Dentistry, University Alberta, Edmonton, Canada Physics, Correspondence to: Pasdar, email: mpasdar@ualberta.ca Tuszynski,...

Desmosomes are one component of the intercellular junctional complex in epithelia. In cultures epithelial cells, desmosome assembly can be regulated by modulating calcium concentrations growth media. At present, very little is known about intracellular signal transduction mechanisms that regulate and disassembly response to changing extracellular concentrations. We have used inhibitors protein kinases phosphatases a combined biochemical morphological approach analyze role phosphorylation...

Bcl-2, a member of the apoptosis-regulating family proteins confers survival advantage on cells by inhibiting apoptosis. Bcl-2 expression is estrogen-responsive and high in various tumors. Overexpression has been associated with loss contact inhibition, unregulated growth foci formation culture. In this study, we have examined effects bcl-2 overexpression cell-cell adhesion MCF-7 MDCK epithelial cell lines respectively. estrogen receptor-positive mammary carcinoma led to decreased surface...

Plakoglobin (γ-catenin) is a homolog of β-catenin with dual adhesive and signaling functions. participates in cell-cell adhesion as component the adherens junction desmosomes whereas its function mediated by interactions various intracellular protein partners. To determine role plakoglobin during tumorigenesis metastasis, we expressed human tongue squamous cell carcinoma (SCC9) cells compared mRNA profiles parental SCC9 their plakoglobin-expressing transfectants (SCC9-PG). We observed that...

Aberrant expression of cadherins and catenins plays pivotal roles in ovarian cancer development progression. Plakoglobin (PG, γ-catenin) is a paralog β-catenin with dual adhesive signaling functions. While has known oncogenic function, PG generally acts as tumor/metastasis suppressor. We recently showed that interacted p53 its growth/metastasis inhibitory function may be mediated by this interaction. Very little about the role cancer. Here, we investigated vitro suppressor effects cell lines...

Abstract We have analyzed the kinetics of synthesis, phosphorylation, and stability soluble insoluble plakoglobin (PG) their interactions with Dsg1 E‐cadherin in Madin‐Darby canine kidney (MDCK) epithelial cells absence cell adhesion after induction cell‐cell contact. Using a combination biochemical morphological approaches, we show that newly synthesized PG enters soluble:insoluble pool proteins 60:40 ratio regardles Following is increasingly found pool. Although contact does not effect...

A strategy based on isotope labeling of peptides and liquid chromatography matrix-assisted laser desorption ionization mass spectrometry (LC−MALDI MS) has been employed to accurately quantify confidently identify differentially expressed proteins between an E-cadherin-deficient human carcinoma cell line (SCC9) its transfectants expressing E-cadherin (SCC9-E). Proteins extracted from each were tryptically digested the resultant labeled individually with either d(0)- or d(2)-formaldehyde. The...

Abstract Plakoglobin (Pg) and β‐catenin are homologous proteins that function in cell–cell adhesion signaling. The cadherin‐associated form of these mediates adhesion, whereas the cytosolic/nuclear has a signaling role. Despite their interactions with common cellular partners, well‐documented oncogenic potential while Pg less characterized tumor suppressor activity. We showed previously overexpression Pg‐deficient SCC9 cells (SCC9‐Pg‐WT) induced Bcl‐2 expression inhibited apoptosis. To...

Nucleophosmin (NPM) is a nucleolar phosphoprotein that involved in many cellular processes and has both oncogenic growth suppressing activities. NPM localized primarily nucleoli but shuttles between the nucleus cytoplasm, sustained cytoplasmic distribution contributes to its tumor promoting Plakoglobin (PG, γ-catenin) homolog of β-catenin with dual adhesive signaling functions. These proteins interact cadherins mediate adhesion, while their activities are regulated by association various...

Cadherins are calcium‐dependent cell adhesion receptors with strong morphoregulatory functions. To mediate functional adhesion, cadherins must interact actin cytoskeleton. Catenins cytoplasmic proteins that the interactions between and In addition to their role in cell–cell catenins also participate signaling pathways regulate growth differentiation. appear be involved melanocyte development transformation. Here, we investigated function of cadherin–catenin complexes normal transformation...

Pg is a homologue of β-catenin and Armadillo, the product Drosophila segment polarity gene has been shown to have both adhesive signaling functions. It interacts with classic desmosomal cadherins. interaction cadherins essential for desmosome assembly. Its precise role in cadherin complexes unclear, although Pg-E–cadherin appears be necessary formation desmosomes. In addition adhesion complexes, number proteins involved regulation cell differentiation proliferation such as Lef-1/Tcf-1...

Tumor suppressor/transcription factor p53 is mutated in over 50% of all cancers. Some mutant proteins have not only lost tumor suppressor activities but they also gain oncogenic functions (GOF). One the most frequently expressed GOF mutants Arg175His (p53