A5072017992- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Acute Lymphoblastic Leukemia research
- Transgenic Plants and Applications
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Protein purification and stability
- Enzyme Catalysis and Immobilization
- Infant Nutrition and Health
- Protein Hydrolysis and Bioactive Peptides
- Biosimilars and Bioanalytical Methods
- Cancer Research and Treatments
- Light effects on plants
- Advanced Drug Delivery Systems
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- biodegradable polymer synthesis and properties
- Skin Protection and Aging
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Biochemical and Structural Characterization
- Biocrusts and Microbial Ecology
- Aquaculture Nutrition and Growth
- Protein Structure and Dynamics
Universidade de São Paulo
2018-2024
University of Genoa
2024
L-asparaginase (ASNase) from Escherichia coli is currently used in some countries its PEGylated form (ONCASPAR, pegaspargase) to treat acute lymphoblastic leukemia (ALL). PEGylation refers the covalent attachment of poly(ethylene) glycol protein drug and it not only reduces immune system activation but also decreases degradation by plasmatic proteases. However, pegaspargase randomly and, consequently, with a high degree polydispersity final formulation. In this work we developed...
The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes the protein, reduce degradation by proteolytic enzymes, enhance long-term stability maintain or even improve pharmacokinetic pharmacodynamics characteristics protein drug. Nonetheless, correct information in terms PEGylation process from reaction downstream processing...
L-Asparaginase (ASNase) is used in the treatment of acute lymphoblastic leukemia, being produced and commercialized only from bacterial sources. Alternative Saccharomyces cerevisiae ASNase II coded by ASP3 gene was biosynthesized recombinant Pichia pastoris MUT s under control AOX1 promoter, using different cultivation strategies. In particular, we applied multistage fed-batch divided four distinct phases to produce determine fermentation parameters, namely specific growth rate, biomass...
L-asparaginase (ASNase) is an important biological drug used to treat Acute Lymphoblastic Leukemia (ALL). It catalyzes the hydrolysis of L-asparagine (Asn) in bloodstream and, since ALL cells cannot synthesize Asn, protein synthesis impaired leading apoptosis. Despite its therapeutic importance, ASNase treatment associated side effects, mainly hypersensitivity and immunogenicity. Furthermore, degradation by plasma proteases immunogenicity shortens enzyme half-life. Encapsulation liposomes,...
Abstract Crisantaspase is an asparaginase enzyme produced by Erwinia chrysanthemi and used to treat acute lymphoblastic leukemia (ALL) in case of hypersensitivity Escherichia coli l ‐asparaginase (ASNase). The main disadvantages crisantaspase are the short half‐life (10 H) immunogenicity. In this sense, its PEGylated form (PEG‐crisantaspase) could not only reduce immunogenicity but also improve plasma half‐life. work, we developed a process obtain site‐specific N‐terminal...
Abstract Skin is the ultimate barrier between body and environment prevents water loss penetration of pathogens toxins. Internal external stressors, such as ultraviolet radiation (UVR), can damage skin integrity lead to disorders. Therefore, health ageing are important concerns increased research from cosmetic pharmaceutical sectors aims improve conditions provide new anti‐ageing treatments. Biomolecules, compared low molecular weight drugs ingredients, offer high levels specificity....
Phage display links the phenotype of displayed polypeptides with DNA sequence in phage genome and offers a universal method for discovery proteins novel properties. However, large multisubunit on phages remains challenge. A majority protein systems are based monovalent phagemid constructs, but methods robust multiple copies scarce. Here, we describe DNA-encoded ∼ 200 kDa tetrameric l-asparaginase M13 fd produced by ligation SpyCatcher-Asparaginase fusion (ScA) PEGylated-ScA (PEG-ScA) to...
Abstract Ultraviolet (UV) radiation from sunlight can damage DNA, inducing mutagenesis and eventually leading to skin cancer. Topical sunscreens are used avoid the effect of UV irradiation, but topical application DNA repair enzymes, such as photolyase, provide active photoprotection by recovery. Here we produced a recombinant Thermus thermophilus photolyase expressed in Escherichia coli , evaluated kinetic parameters bacterial growth kinetics stability enzyme. The maximum biomass (𝑋 𝑚𝑎𝑥 )...
The development of fish oral vaccines is great interest to the aquaculture industry due possibility rapid vaccination a large number animals at reduced cost. In previous study, we evaluated effect alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing immune response Atlantic salmon (Salmo salar). this seek characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, optimize microencapsulation...
Thermostability is an important and desired feature of therapeutic proteins critical for the success or failure protein drugs development. It can be increased by PEGylation—binding poly(ethylene glycol) moieties—or glycosylation—post-translational modification to add glycans. Here, thermostability thermodynamic parameters native, PEGylated, glycosylated versions antileukemic enzyme crisantaspase were investigated. First-order kinetics was found describe irreversible deactivation process....