A5006841368- Slime Mold and Myxomycetes Research
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Medawar Building for Pathogen Research
2024-2025
University of Oxford
1965-2025
University of the West of England
2014-2023
Bristol Robotics Laboratory
2017-2018
At Bristol
2015
University of Alabama at Birmingham
1999-2012
Matrix Research (United States)
1997-1999
John Wiley & Sons (United States)
1995
Harvard University
1984-1994
University of Turku
1994
Clones of embryonic chick chondrocytes have been isolated and collagen biosynthesis has followed as the clones grow eventually lose division capacity. Analysis type at each successive subculture until time cellular senescence shown that a change in synthesis occurs from cartilage-specific Type II (chain composition [alpha1(II)]3) to mixture I [alpha1(I)2alpha2) trimer composition[alpha1(I)]3). The results demonstrate unequivocally expression chondrocyte phenotype is unstable vitro, previous...
Type IX collagen is a recently described component of cartilage. This nonfibril-forming molecule composed three helical (COL1-COL3) and four nonhelical domains (NC1-NC4) also serves as core protein for single glycosaminoglycan side chain. To understand its spatial relationship with other matrix constituents, peptide containing lysine-derived cross-link was isolated subjected to amino acid sequencing. The results show that type linked II by hydroxypyridinium cross-link. connects the central...
Integrins α<sub>1</sub>β<sub>1</sub> and α<sub>2</sub>β<sub>1</sub> are two major collagen receptors on the surface of eukaryotic cells. Binding to is primarily due an A-domain near N terminus α chains. Previously, we reported that recombinant α<sub>1</sub>β<sub>1</sub>(α<sub>1</sub>A) had at least affinity classes binding sites in type I (Rich, R. L., <i>et al.</i> (1999)<i>J. Biol. Chem.</i> 274, 24906–24913). Here, compared (α<sub>2</sub>A) with α<sub>1</sub>A using plasmon resonance...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMinor collagens of chicken hyaline cartilageCharles A. Reese and Richard MayneCite this: Biochemistry 1981, 20, 19, 5443–5448Publication Date (Print):September 15, 1981Publication History Published online1 May 2002Published inissue 15 September 1981https://pubs.acs.org/doi/10.1021/bi00522a014https://doi.org/10.1021/bi00522a014research-articleACS PublicationsRequest reuse permissionsArticle Views128Altmetric-Citations161LEARN ABOUT THESE...
Previous studies show that the collagen fibrils of mammalian vitreous humor are assembled largely from type II with smaller amounts IX and either V or XI collagen. In this paper, we report separation two chains V/type by heparin-Sepharose chromatography. These were characterized sequencing selected cyanogen bromide tryptic peptides subsequent comparison these sequences cDNA-derived amino acid alpha 1(V), 1(XI), 2(V), 2(XI) chains. The results molecules containing 1(XI) 2(V) results, together...
We present a detailed analysis both of tryptic peptides and amino-terminal sequences the subunits two collagenous fragments (HMW LMW) previously isolated from pepsin extracts chicken cartilage (Reese, C.A., Mayne, R. (1981) Biochemistry 20, 5443-5448). This comparison with nucleotide sequence cDNApYN1738 (Ninomiya, Y., Olsen, B.R. (1984) Proc. Natl. Acad. Sci. U. S. A. 81, 3014-3018) shows that HMW LMW are pepsin-resistant unique collagen composed molecules three different polypeptide chains...
Chick embryo chondrocytes, in primary culture, initially synthesize only Type ii collagen (chain composition, [alpha1(II)13), as judged by two criteria: (i) carbosymethyl-cellulose chromatography of the denatured collagen, and (ii) cyanogen bromide peptides derived from isolated chains. After a period growth 5-bromo-2'-deoxyuridine, however, synthesis different types could be detected after differential salt precipitation newly synthesized native collagens neutral solutions at 2.2 M NaCl...
Two monoclonal antibodies have been produced against chick type V collagen and shown to be highly specific for separate, conformational dependent determinants within this molecule. When used immunocytochemical tissue localization, these show that a major site the in situ deposition of is extracellular matrices many dense connective tissues. In these, however, it largely form unavailable antibodies, thus requiring "unmasking" treatment obtain successful staining. The specificity two IgG was...
We have discovered a new member of the class I small leucine-rich repeat proteoglycan (SLRP) family which is distinct from other SLRPs since it possesses unique stretch aspartate residues at its N terminus. For this reason, we called molecule asporin. The deduced amino acid sequence about 50% identical (and 70% similar) to decorin and biglycan. However, asporin does not contain serine/glycine dipeptide required for assembly O-linked glycosaminoglycans probably proteoglycan. tissue expression...
We have generated transgenic mice harboring a glycine-to-cysteine mutation in residue 85 of the triple helical domain mouse type II collagen. The offspring different founders displayed phenotype severe chondrodysplasia characterized by short limbs and trunk, cranio-facial deformities, cleft palate. affected pups died acute respiratory distress caused an inability to inflate lungs at birth. Staining skeleton showed retardation growth for practically all bones. Light microscopic examination...
The C-terminal G domains of laminin α chains have been implicated in various cellular and other interactions. domain the α4 chain was now produced transfected mammalian cells as two tandem arrays LG modules, α4LG1–3 α4LG4–5. recombinant fragments were shown to fold into globular structures could be distinguished by specific antibodies. Both able bind heparin, sulfatides, microfibrillar fibulin-1 fibulin-2. They were, however, poor substrates for cell adhesion had only a low affinity...
Five different collagen chains and one smaller collagenous fragment have been isolated from the collagens found in combined cell layer medium of rhesus monkey aortic smooth muscle cultures. The which can be identified are alpha1 (III), alpha1(I), alpha2, A B. peptide exhibits an apparent molecular weight 45 000 has designated CP45 (Mayne, R., et al. (1977), Arch. Biochem. Biophys. 181, 462). Smooth cells continue to synthesize these components derived for at least eight passages culture. At...
ABSTRACT To examine the regulation of collagen types EX and X during hypertrophic phase endochondral cartilage development, we have employed in situ hybridization immunofluorescence histochemistry on selected stages embryonic chick tibiotarsi. The data show that mRNA for type appears at or about time detect first appearance protein. This result is incompatible with translational regulation, which would require accumulation to occur an appreciably earlier time. Data later-stage embryos...