A5002449943- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant and animal studies
- Polysaccharides and Plant Cell Walls
- Genomics and Chromatin Dynamics
- Seed Germination and Physiology
- Mitochondrial Function and Pathology
Wuhan University
2016-2024
Xiamen University
2024
During angiosperm male gametogenesis, microspores divide to produce a vegetative cell (VC) and germline (MG), each with distinct fates. The mechanism underlying determination of the MG cell/VC fate remains an important area research, many unanswered questions. Here, we report that H3K27me3 is essential for VC commitment in Arabidopsis thaliana gametophytes; erasure contributes initiation. VC-targeted disturbed development shifted toward gamete destination, which suggests cells require...
Most pentatricopeptide repeat (PPR) proteins are involved in organelle post-transcriptional processes, including RNA editing. The PPR include the PLS subfamily, containing characteristic triplets of P, L, and S motifs; however, their editing mechanisms roles developmental processes not fully understood. In this study, we isolated Arabidopsis thaliana Growing slowly 1 ( AtGRS1 ) gene showed that it functions plant development. null mutants grs1 exhibit slow growth sterility. Further analysis...
Summary Rice fertility is critical for rice reproduction and thus a focus of interest. Most studies have addressed male sterility its relation to production. The mechanisms regulation embryogenesis endosperm development are essential reproduction, but remain largely unknown. Here, we report functional analysis the gene Os GCD 1 , which encodes highly conserved homolog Arabidopsis ( GAMETE CELLS DEFECTIVE 1). mutants were generated using CRISPR /Cas9 system subjected analysis. homozygote...
Abstract Plant embryos are generated and develop in a stable well-protected microenvironment surrounded by maternal tissue, which is vital for embryogenesis. However, the signaling mechanisms responsible tissue-to-proembryo communication not well understood. Here, we report pathway communication. We identify DELLA protein, NtCRF1 ( NtCYS regulative factor 1), regulates suspensor programmed cell death (PCD). can bind to promoter of regulate PCD-switch module NtCYS-NtCP14 response gibberellin...
Seed development is one of the extensively investigated fields plant developmental biology due to its great agricultural and economic value. <italic>Arabidopsis thaliana</italic> as a model has been widely employed in seed research over past 30 years these studies provide vast information involving various aspects development, which have greatly enhanced our understanding critical processes development. As it very difficult summarize all advances this field, present review we focussed on key...
Abstract Body axis establishment is one of the earliest patterning events in plant embryogenesis. Asymmetric zygote division critical for apical–basal formation Arabidopsis (Arabidopsis thaliana). However, how orientation cell plane regulated and its relation to proper position embryos grasses remain poorly understood. By characterizing mutants 3 rice (Oryza sativa) WUSCHEL HOMEOBOX9 (WOX9) genes, whose paralogs play essential roles zygotic asymmetric fate determination, we found 2 kinds...
Plant embryogenesis begins with an asymmetric division of the zygote, producing apical and basal cells distinct cell fates. The zygote is thought to be critical for embryo pattern formation; however, molecular mechanisms regulating this process, especially maintaining accurate position proper orientation plane, remain poorly understood. Here, we report that a dynamin-related protein in Nicotiana tabacum, NtDRP, plays role zygotic plane. Down-regulation NtDRP caused occur different, incorrect...
We previously reported that a novel motor protein belonging to the kinesin-12 family, NtKRP, displays critical roles in regulating embryo and seed size establishment. However, it remains unknown exactly how NtKRP contributes this developmental process. Here, we report 60S ribosomal NtRPL17 directly interacts with NtKRP. The phenotypes of RNAi lines show notable reduction. Structural observations NtRPL17-silenced embryos/seeds reveal reduction is due decrease cell number. In these embryos,...
Summary During male gametogenesis in cereals, the generative cell undergoes a positioning process that parallels dynamics of central vacuole, which is believed to be associated with movement gametophyte. However, impact and vacuole on has remained poorly understood. Here, we report Os GCD 1 ( GAMETE CELLS DEFECTIVE ) dysfunction influenced pollen development disrupted germination. Loss function altered was mispositioned. Nevertheless, twin sperm cells were generated normally, indicating does...
Abstract During angiosperm male gametogenesis, microspores divide to produce a vegetative cell (VC) and germline (MG), each with distinct fate. How the MG cell/VC fate is determined remains largely unknown. Here, we report that H3K27me3 essential for VC commitment erasure contributes initiation in gametophyte of Arabidopsis . The VC-targeted disturbed development resulted shifting towards gamete destination, which suggests cells require triggering Multi-omics cytologic analysis confirmed...