A5081075069- Parasitic infections in humans and animals
- Congenital Anomalies and Fetal Surgery
- Parasitic Infections and Diagnostics
- Parasite Biology and Host Interactions
- Toxoplasma gondii Research Studies
- Amoebic Infections and Treatments
- Climate Change, Adaptation, Migration
- Urological Disorders and Treatments
University of Zurich
2020-2025
ZHAW Zurich University of Applied Sciences
2024-2025
University of Bern
2019-2023
Human cystic and alveolar echinococcosis are among the priority neglected zoonotic diseases for which WHO advocates control. The incidence of both has increased substantially in past 30 years Kyrgyzstan. Given scarcity adequate data on local geographical variation these focal diseases, we aimed to investigate within-country at a high spatial resolution Kyrgyzstan.We mapped all confirmed surgical cases reported through national surveillance system Kyrgyzstan between Jan 1, 2014, Dec 31, 2016,...
Infection of humans by the larval stage tapeworms Echinococcus granulosus sensu lato or multilocularis causes life-threatening zoonoses cystic echinococcosis (CE) and alveolar (AE). Although liver lesions are a hallmark both diseases, course, prognosis, patients’ management decisively differ between two. The wide overlapping spectrum morphologies limited availability ancillary tools challenges for pathologists to reliably diagnose subtype echinococcosis. Here, we systematically...
Aims Infections by the larval stage of tape worms Echinococcus multilocularis and granulosus s.l . are potentially fatal zoonoses affecting humans as dead‐end hosts. Histopathological evaluation hepatic echinococcosis is an integral part patient management, including distinction between alveolar (AE) cystic (CE), which associated with different disease courses treatments. To improve histopathological assessment lesions, we aimed to develop robust criteria evaluate their viability decay....
Alveolar echinococcosis (AE) caused by Echinococcus multilocularis , is a severe zoonotic disease in humans. One of the major metacestode antigens E. Em2 or Em2(G11) native purified antigen. The antigen used for serological and histopathological diagnosis AE humans plays an important role parasite–host interactions. As mucin-type glycosylated protein, protein backbone has not been identified yet. We have targeted identification through mass spectrometry (LC-MS/MS) analysis result, we...
Alveolar and cystic echinococcosis (AE, CE) caused by E. multilocularis granulosus s.l., respectively, are considered emerging zoonotic diseases in Kyrgyzstan with some of the world highest regional incidences. Little is known regarding molecular variability both species Kyrgyzstan. In this study we provide data from a total 72 parasite isolates derived humans (52 AE 20 CE patients) 43 samples dogs (23 infected s.l.).Genetic was studied using concatenated complete sequences cob, nad2 cox1...
Both alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE 64 CE patients that were diagnosed in Switzerland or Kyrgyzstan. Overall, 10 native Echinococcus spp. antigens, 3 recombinant 4 commercial assays comparatively evaluated. All E. multilocularis antigens produced duplicates with a European Kyrgyz isolate showed...
Alveolar (AE) and cystic echinococcosis (CE) are severe parasitic zoonoses caused by the larval stages of Echinococcus multilocularis E. granulosus sensu lato , respectively. A panel 7 monoclonal antibodies (mAbs) was selected against major diagnostic epitopes both species. The binding capacity mAbs to spp. excretory/secretory products (ESP) analyzed sandwich-ELISA, where mAb Em2G11 EmG3 detected in vitro extravesicular ESP s.s. These findings were subsequently confirmed detection...
Different helminths and protozoa are transmitted to humans by oral uptake of environmentally resistant parasite stages after hand-to-mouth contact or contaminated food water. The aim this study was develop validate a method for the simultaneous detection from fresh produce (lettuce) one-way isolation test kit followed genetic identification (PCR, sequencing). Three sentinel zoonotic agents (eggs Toxocara canis, Echinococcus multilocularis oocysts Toxoplasma gondii) were used investigate...
Objectives Alveolar echinococcosis (AE) is an orphan zoonosis of increasing concern in endemic areas, including Europe. It frequently presents advanced, inoperable stage, that requires life-long parasitostatic benzimidazole therapy. In some patients, long-term therapy leads to negative anti-Em18 antibody ELISA and PET. disputed, whether these patients are truly cured treatment can be safely discontinued. Our aim was retrospectively assess outcome 34 with AE who participated a previous study...
Abstract The metacestode of Echinococcus multilocularis is the etiological agent alveolar echinococcosis. stage used for research maintained in rodents by serial passages. In order to determine whether cryopreservation E. metacestodes would be suitable long-term maintenance and replace passages, isolates different geographic origin were cryopreserved 1984–1986. aim current study was test viability following (up 35 years) phylogenetic clades these belonged to. Cryopreserved tested vitro vivo...
The detection of Echinococcus multilocularis in infected canids and the environment is pivotal for a better understanding epidemiology alveolar echinococcosis endemic areas. Necropsy/sedimentation counting technique remain gold standard canid infection. PCR-based methods have shown high sensitivity specificity, but they been hardly used large scale prevalence studies. Loop-mediated isothermal amplification (LAMP) fast simple method to detect DNA with having potential field-application. A...
Cystic Echinococcosis (CE) is a zoonotic disease caused by the larval stage of tapeworm Echinococcus granulosus sensu lato (s.l.). This study aims to investigate use two monoclonal antibodies (mAbEmG3 and mAbEm2G11) immunohistochemistry (IHC) confirm diagnosis CE in human patients, particular those cases which other techniques fail provide correct or conclusive diagnosis. For this purpose, survey on 13 patients was performed. These subjects were referred Sardinian hospitals (Italy) from 2017...
Abstract Recently, there have been epidemics of human cystic echinococcosis (CE) and alveolar (AE) in Kyrgyzstan. This study investigated 2 districts for the presence Echinococcus granulosus s.l. multilocularis eggs; species identity was confirmed by polymerase chain reaction dog feces level environmental contamination with parasite eggs 2017–2018 also investigated. In Alay district 5 villages a high reported annual incidence AE 162 cases per 100 000 Kochkor which had much lower 21 were...
Abstract Susceptibility to Echinococcus multilocularis infection considerably varies among intermediate (mostly rodents) and dead-end host species (e.g. humans pig), in particular regarding intestinal oncosphere invasion subsequent hepatic metacestode development. Wistar rats are highly resistant diseases upon oral inoculation with E. eggs, however, after immunosuppressive treatment dexamethasone, become susceptible. To address the role of cellular innate immunity, were individually or...