A5024544931- Inflammatory Bowel Disease
- Fatty Acid Research and Health
- Microscopic Colitis
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Peptidase Inhibition and Analysis
- Clinical Nutrition and Gastroenterology
- Macrophage Migration Inhibitory Factor
- Lipid metabolism and disorders
- Pancreatitis Pathology and Treatment
- Cancer Research and Treatments
- Liver Disease and Transplantation
- Renin-Angiotensin System Studies
- Infant Nutrition and Health
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Hemoglobinopathies and Related Disorders
- Enhanced Recovery After Surgery
- Gut microbiota and health
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Probiotics and Fermented Foods
- Metabolism and Genetic Disorders
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2011-2022
Newbiotechnic (Spain)
2007
Hospital Universitari Germans Trias i Pujol
2002-2007
Intravenous ciclosporin is considered to be the only alternative avoid surgery in severe, steroid-refractory ulcerative colitis. In responders, some authors recommend a switch oral act as 'bridge' until therapeutic action of azathioprine achieved for maintenance treatment.To report short- and long-term outcome intravenous ciclosporin-responsive colitis patients treated with without ciclosporin.The records all were reviewed. Responders following treatment but therapy included. Patients...
<i>Background/Aims:</i> Glomerular kidney disease (GKD) is suspected in patients based on proteinuria, but its diagnosis relies primarily renal biopsy. We used urine peptide profiling as a noninvasive means to link GKD-associated changes each glomerular entity. <i>Methods:</i> Urinary profiles of 60 biopsy-proven and 14 controls were analyzed by combining magnetic bead enrichment, MALDI-TOF MS analysis, ClinProTools v2.0 select differential peptides. Tentative...
Several studies have suggested that the partially fermentable fibre Plantago ovata husk (PO) may a protective effect on colorectal cancer (CRC). We studied potentially pro-apoptotic of PO and implicated mechanisms in CRC cells with different molecular phenotypes (Caco-2, HCT116, LoVo, HT-29, SW480) after anaerobic fermentation colonic bacteria as it occurs human colon. The products induced apoptosis all primary tumour metastatic cell lines, independent p53, adenomatous polyposis coli,...
Gas chromatographic-mass spectrometric (GC/MS) tracking of stable-isotope-labeled substrates is useful in metabolic studies. However, GC/MS analysis long-chain fatty acid methyl esters yields results that mostly depend on their concentration the system. We describe a protocol aimed to obviate this and other drawbacks plasma [1-(13)C]palmitic [1-(13)C]oleic measurements.Lipoproteins were separated by sequential ultracentrifugation. Free or esterified heptadecanoic was used as internal...
This work illustrates the practical use of combined microbore reversed-phase high-performance liquid chromatography (RP-HPLC) with electrospray ionization mass spectrometry (ESI-MS) in protein identification. The approach consisted detection abnormal beta-globin chain by ESI-MS analysis mixtures intact globins, which simultaneously provided their molecular masses. Separation polypeptide globin chains was carried out using C4 RP-HPLC on-line ESI-MS. Direct peptide-mapping without previous...
Previous studies suggested that Interleukin-10 (IL-10) depletion in Crohn's disease (CD) could predict outcome. To determine IL-10 blood and at different intestinal locations patients with active CD to assess its potential prognostic capacity identify aggressive CD. Twenty-three were included. Ulcerative colitis (UC), infectious healthy individuals acted as controls. Serum mucosal samples taken baseline 1 month after steroid initiation patients. Patients classified according response....
Abstract Background The ω3 derivate resolvin D1 (RvD1), and the ω6 dihomo-γ-linolenic acid (DGLA) prevent collateral damage by overcoming inflammation enhancing microbial clearance. In Crohn's disease, mesenteric adipose tissue becomes inflamed hyperplasies due to transmural lesions bacterial translocation, developing so-called creeping fat. This is unable clear has been linked severer intestinal post-operative recurrence (POR). Recently, we have described presence of DGLA increase in ω6/ω3...
Rationale: Standard rodent diets mostly contain n6 fatty acids (n6), which are proinflammatory and may contribute to perpetuate the experimental colitis in these animals.We previously found that dietary MCT downregulate some inflammatory mediators rats.We aimed assess 1) effect of replacing 50% fat by on course IL10(-/-) mice, 2) molecular/cellular mechanisms involved.Methods: housed pathogen-free environment, were fed with 18% energy as fat, 100% sunflower oil (n6; n=12) or oil/50% coconut...