A5016819566- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Mast cells and histamine
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Diabetes and associated disorders
- Toxin Mechanisms and Immunotoxins
- Platelet Disorders and Treatments
- Aortic aneurysm repair treatments
- Vascular Procedures and Complications
- Innovations in Medical Education
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Computational Drug Discovery Methods
- Receptor Mechanisms and Signaling
- Genomics and Rare Diseases
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Neuroscience and Neural Engineering
- Liver physiology and pathology
- Takotsubo Cardiomyopathy and Associated Phenomena
- Allergic Rhinitis and Sensitization
- Herpesvirus Infections and Treatments
- Pigment Synthesis and Properties
- Colorectal Cancer Treatments and Studies
- Cardiovascular and Diving-Related Complications
Cleveland Clinic
2023-2024
National Institute of Pharmaceutical Education and Research
2022
University of Arkansas for Medical Sciences
2013-2021
Johns Hopkins Medicine
2019
Johns Hopkins University
2018-2019
VA Boston Healthcare System
2009-2016
Crawley Hospital
2014
Boston University
2009
VA NY Harbor Healthcare System
2000-2005
New York University
1982-2000
Osteoid osteoma is a benign, self-limited tumor of bone that usually requires surgical excision for relief pain and to prevent long-term consequences. Radio-frequency electrodes have been successfully safely used ablate small areas the central nervous system perform ablation elsewhere in body. The authors this technique four patients with proved or presumptive osteoid osteoma, completely relieving symptoms three.
Background: Transthyretin (TTR) V122I (rs76992529) is one of 111 variants caused by point mutations in the coding sequence human TTR gene that are associated with systemic amyloidosis. It results from a G to A transition at CG dinucleotide codon 142(122 mature protein) and has been described almost exclusively people African descent. Several series have reported allele frequencies 0.015 0.020 African-Americans.Objective: To define more accurately frequency variant African-American...
The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via DNA sequence encoding short collagen linker, and tripartite fusion protein expressed in Escherichia coli. Site-specific mutagenesis used change glutamic acid residue 177 aspartic or alanine. When mutant proteins were expressed, purified, tested quantitatively enzymatic activity, carboxylate function at position found not be absolutely essential A-chain catalysis.
Human gamma heavy chain disease (HCD) is characterized by the presence in serum of a short monoclonal Ig unattached to light chains. Although most HCD proteins have internal deletions, some defect NH2-terminal. The OMM 3 protein latter type, having undergone an extensive NH2-terminal deletion with sequence starting within hinge. A cell line synthesizing has enabled us study biogenesis abnormal molecule. In vitro translation isolated mRNA yields containing hydrophobic leader sequence. intact...
Abstract Histamine release from the leukocytes of ragweed sensitive donors, which also well to Fc-specific anti-IgE, was studied. In one type experiment effect increasing concentrations monomer anti-IgE (Fab) upon induced by observed. for a suboptimum concentration inhibited histamine release. However, supraoptimum enhanced Evidence obtained shows that render cells unresponsive near optimum antigen E. As were increased into inhibitory region due E not maintained but dropped in parallel...
Background Transthyretin (TTR) pV142I (rs76992529-A) is one of the 113 variants in human TTR gene associated with systemic amyloidosis. It results from a G to A transition at CG dinucleotide codon for amino acid 122 mature protein (TTR V122I). The allele frequency 0.0173 African Americans. Methods PCR-based assays genotype 2767 DNA samples obtained participants genetic studies various populations supplemented sequencing data 529 within 1000 Genomes Project. Results rs76992529-A variant was...
A genomic clone was isolated from a human lymphoid cell line which synthesized an NH2-terminally deleted gamma 3 heavy chain disease protein. Nucleotide sequence analysis revealed normal 310 bp 5' to the initiator ATG through codon for VH amino acid 14. Amino 15 derived last J4 acid. Thus, contained deletion of codons region beyond 14, as well those entire D and most J coding region. Some abnormalities were observed in 400 after deletion. Beyond this, there excellent homology published...
The stable expression of the human cytochrome CYP2E1 (P450 alcohol) was performed in mammalian cell line PC-12. This expressed b5 (58 +/- 12 pmol/mg microsomal protein vs 528 80 liver) and a high level NADPH: P450 reductase (140 20 nmol.min-1.mg protein-1 68 48 nmol.min-1.mg-1 liver). An plasmid constructed using cDNA for mRNA Rous sarcoma virus (RSV) promoter. co-transfected with RSVneo into PC-12 cells. Clones were selected resistance to neomycin analog, G418, then screened isozyme by...
Lymph node (LN) involvement predicts recurrence in patients who have undergone resection of apparently localized nonsmall cell lung carcinoma (NSCLC). Standard detection methods for LN disease a low sensitivity, and many with apparent N0 status develop recurrent disease. Molecular techniques can improve the micrometastases, whereas sentinel lymph (SLN) mapping indicate which may contain micrometastases. These methods, although potentially complementary, not, to authors' knowledge, been used...
The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed disaccharide repeating units<mml:math...
A high density of binding sites for the ligands 3H-SCH-23390 and 3H-SKF-83566 has been found in choroid plexus. Although these have similar pharmacology to D1 dopamine receptors, high-affinity component plexus identified as 5-HT1c subtype serotonin receptor. We investigated possible role receptors modulating production cerebrospinal fluid (CSF) rats. (R) SCH-23390 produced up a 50% decrease net CSF production, compared saline. This compound is partial agonist at an antagonist receptors. The...
Abstract The in vitro histamine release by washed leukocytes from rabbits immunized to produce reagin specific for the benzyl penicillolyl (BPO) and 2,4 dinitrophenyl (DNP) haptenic groups was studied. animals were one of (DNP or BPO) both BPO). divalent univalent haptens used initiate bis-DNP-octamethylenediamine (di-DNP), DNP-n-octylamine (uniDNP), bis-BPO-octamethylenediamine (diBPO), BPO-n-octylamine (uniBPO) N-DNP,N-BPO, octamethylenediamine (mixed BPO-DNP). data indicate that 1)...
Lymphoid cells obtained from the peripheral blood of a patient with heavy chain disease have been established in long-term culture. They continue to produce protein antigenically identical deleted gamma3 found patient's serum. The availability cell line has made it possible analyze mRNA coding for this protein. primary vitro translation product is 1500-2000 daltons larger than polypeptide portion cytoplasmic or secreted and methionine at amino terminus. sediments 15.5 S on sucrose gradients...
Abstract Allergic histamine release from rabbit platelets can be initiated through the alternate pathway of C activation. The reaction proceeds in absence Fc portion antibody molecule. time course due to this is slower than that after activation classical sequence. Platelet injury immune complexes both pathways requires sixth component.
Double-stranded (ds)RNA in the infected cells is a trait shared by most if not all viruses. While humans have developed variable immune responses, viruses also counter-measures to defeat dsRNA-induced antiviral strategies. Thus, we proposed broad strategy antagonize countermeasures of and bypass signals that are readily defeated By rewiring dsRNA-binding proteins dsRNA complex reconnecting them apoptosis signaling, created several dsRNA-dependent caspase recruiters, termed dsCAREs, would...
Cellular and molecular mechanisms regulating the activity of sodium pump or Na,K-ATPase during proliferation hepatocytes following 70% liver resection have not been defined. may be regulated by synthesis its α- β-subunits, sorting to either sinusoidal apical plasma membrane domains, increasing lipid fluidity. This study investigated time course changes hepatic regeneration for activity, composition fluidity, protein content subfractions. As early as 4 h after resection, was increased...
Sterilizing properties of formaldehyde in anhydrous methanol and water were compared. Formaldehyde achieved sterilization 24 hr. was found to sterilize shorter periods time have a longer shelf life than methanol.
TBL enables a group of learners to engage in independent preparation through prereadings, check their knowledge with readiness assessments, and both share build on personal experience each topic robust small-group discussions. This exercise uses case-based questions enable make treatment plan for challenging cases hypertension manage chronic kidney disease (CKD) the primary care setting.This module serves as part series team-based learning (TBL) modules an internal medicine residency...