The most primitive engrafting hematopoietic stem cell has been assumed to have a fixed phenotype, with changes in engraftment and renewal potential occurring stepwise irreversible fashion linked differentiation. Recent work shows that vitro cytokine stimulation of murine marrow cells induces cycle transit cells, taking 40 h for progression from G0 mitosis 12 subsequent doublings. At 48 culture, progenitors are expanded, but is markedly diminished. We investigated whether this effect on was...

This retrospective study identified prognostic factors associated with survival; and compared survival data in 94 canine mammary carcinoma (MCA) dogs treated surgery (n = 58), or adjunct chemotherapy 36), a subset of poor factors. On multivariate analysis independent predictors median time (MST) were clinical stage, lymphatic invasion (LI; present 179 days; none 1098 days), ulceration (present 118 443 days) surgical margins (incomplete 70 complete 872 days). Complete MST stages 1-3 MCA 68 LI...

Forty‐three dogs with lymphoma that had relapsed or failed to achieve complete remission previous chemotherapy were treated lomustine (1‐(2‐chloroethyl)‐3‐cyclohexyl‐l‐nitrosourea [CCNU]) at a dosage of 90–100 mg/m 2 body surface area PO every 3 weeks. Durable partial responses occurred in 11 for median 86 days. The acutely dose‐limiting toxicosis was neutropenia 7 days after administration, resulting recommended 90 . Cumulative thrombocy‐topenia receiving continued CCNU treatment, and dose...

Forty-three dogs with lymphoma that had relapsed or failed to achieve complete remission previous chemotherapy were treated lomustine (1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea [CCNU]) at a dosage of 90-100 mg/m2 body surface area p.o. every 3 weeks. Durable partial responses occurred in 11 for median 86 days. The acutely dose-limiting toxicosis was neutropenia 7 days after administration, resulting recommended 90 mg/m2. Cumulative thrombocytopenia receiving continued CCNU treatment, and...

Eighty-two dogs with lymphoma received a single 15-week course of chemotherapy, after which treatment was ceased until relapse. Fifty-six (68%) achieved complete remission for median 1st duration 20 weeks. Forty-eight relapsed, 30 repeated the induction cycle. In 22 these dogs, had been short, and they maintenance chemotherapy; other 8 2 or 3 cycles chemotherapy. Second rate 87% (26 dogs). Overall disease control 38 that remained on protocol 44 weeks, not markedly shorter than treated...

Ninety-eight dogs with lymphoma treated a 5-drug combination chemotherapy regimen (vincristine, L-asparaginase, cyclophosphamide, doxorubicin, prednisone [VELCAP-L]) were evaluated for pretreatment characteristics predictive response and remission duration. The complete rate was 69%, median duration of 55 weeks. Dogs advanced stage disease, constitutional signs, that older, dyspneic less likely to achieve remission. Once in remission, small without thrombocytopenia have longer Toxicoses...

Thirty‐eight cats with lymphoma were treated vincristine, cyclophosphamide, and prednisone (COP). They randomized at entry to receive maintenance chemotherapy consisting of either single‐agent doxorubicin or continued COP therapy, starting on week 4 treatment continuing for 6 months until relapse. Eighteen achieved complete clinical remission after induction chemotherapy. The median duration 11 was 83 days, which significantly shorter than 7 that received (281 days). Thus, should be...

Thirty-seven dogs with moderately differentiated, cutaneous mast cell tumors had incomplete surgical excisions as determined by histopathology, but no gross evidence of tumor. All were irradiated to a total dose between 46.2 and 48.0 Gy using either an orthovoltage source (n = 20) or linear accelerator (megavoltage) 17). Radiation was delivered area bordered margins 3 cm greater around the scar. The not recurred in 97% one year after radiation therapy 93% three years radiation. Both...

To determine the prognostic factors for survival and tumor recurrence in dogs with cutaneous mast cell tumors (MCTs) perineal inguinal regions treated surgically or without adjunctive radiation therapy, chemotherapy, both.Retrospective study.68 dogs.Medical records of histologically confirmed MCTs region, both were reviewed.Mean tumor-free interval was 1,635 days (median not reached), 1- 2-year rates 79% 71%, respectively. Median time 1,111 (mean, 1,223 days), 61%, Factors that negatively...

Five Rottweiler puppies from 3 unrelated litters developed inspiratory stridor at 11–13 weeks of age. Physical examination disclosed tetraparesis in all dogs, and bilateral lenticular cataracts 4 dogs. Laryngeal under light anesthesia showed laryngeal paralysis Electrodiagnostic testing revealed denervation potentials the distal appendicular muscles dogs tested intrinsic 2 tested. Motor nerve conduction velocity was slightly low 1 dog. Neurogenic muscular atrophy found (n = 3) 2),...

OBJECTIVE To determine histologic and clinical factors associated with survival time in dogs stage II splenic hemangiosarcoma treated by splenectomy a chemotherapy protocol which an anthracycline was alternated lomustine. DESIGN Retrospective case series. ANIMALS 30 hemangiosarcoma. PROCEDURES Medical records of 3 facilities were reviewed to identify for between June 2011 October 2014. Information collected included signalment, disease staging data, whether anemia present, date splenectomy,...

Abstract Published outcomes for dogs with specifically high‐grade mast cell tumours (MCTs), controlled clinical stage, are few. Clinical 49 Kiupel high‐grade, stage I, cutaneous MCTs were evaluated. Median survival time (MST) was 1046 days; 1 and 2‐year rates 79.3% 72.9%, respectively. At study end 24 had died, 23 alive (median follow‐up 980 days) 2 lost to follow‐up. Death considered MCT‐related in 14 of 20 a known cause death. Local tumour recurrence developed nine (18.4%); regional lymph...

Twenty‐eight dogs with lymphoma were treated a 12‐week, 5‐drug chemotherapy protocol concluding high‐dose cyclophosphamide supported by autologous bone marrow transplants. A dose escalation design was used to determine the maximum tolerated (MTD) in this setting. Three levels given: 300 mg/ m 2 IV (group1, 3 dogs), 400 mg/m (group 2, 12 and 500 3, 13 dogs); MTD IV. Toxicity common but mild, dose‐limiting toxicity myelosuppression, specifically neutropenia. No dog died as result of...

Twenty-eight dogs with lymphoma were treated a 12-week, 5-drug chemotherapy protocol concluding high-dose cyclophosphamide supported by autologous bone marrow transplants. A dose escalation design was used to determine the maximum tolerated (MTD) in this setting. Three levels given: 300 mg/ m2 IV (groupl, 3 dogs), 400 mg/m2 (group 2, 12 and 500 3, 13 dogs); MTD IV. Toxicity common but mild, dose-limiting toxicity myelosuppression, specifically neutropenia. No dog died as result of...

Canine appendicular osteosarcoma is an important clinical problem in veterinary medicine. Current standard therapy includes amputation followed by chemotherapy, which improves outcomes; however the percentage of long-term survival still relatively low at 15-20%. Established prognostic factors include serum alkaline phosphatase level, histologic grade, and lymphocyte monocyte counts. We used a protocol with shorter inter-treatment intervals than standard, but we expected to be well-tolerated,...

Haematopoietic stem cells (HSCs) have been extensively characterized regarding in vivo engraftment, surface epitopes and genetic regulation. However, little is known about the homing of these rare cells, their intrinsic motility membrane deformation capacity. We used high‐speed optical‐sectioning microscopy inverted fluorescent videomicroscopy to study highly purified murine lineage‐negative, rhodamine‐low, Hoechst‐low HSCs over time under various vitro conditions. discovered extremely rapid...

Splenic fibrohistiocytic nodules (SFHN) are commonly diagnosed. It is suspected that these represent a heterogeneous group of malignant and nonmalignant diseases, separation which could improve the ability clinicians to prognosticate for dogs.Immunohistochemistry will differentiate histologic diagnoses within SFHN; survival after splenectomy associated with those types.Thirty-two dogs SFHN treated by or under direction from veterinary oncologists.Retrospective case record analysis followed...

Background Prognosis associated with lymphoma in horses is poorly characterized, and treatment often palliative. Long‐term outcome after chemotherapy for not well documented. Objective To report long‐term of treated chemotherapy. Animals Fifteen equids. Methods Retrospective case series. Medical record search call cases on the ACVIM listserv lymphoma. Results adequate data were identified. Complete remission was achieved 5 (33.3%), partial response 9 equids (60%), stable disease 1 horse....

OBJECTIVE To determine the incidence of sterile hemorrhagic cystitis (SHC) in tumor-bearing dogs concurrently treated with oral metronomic cyclophosphamide chemotherapy and furosemide. DESIGN Retrospective case series. ANIMALS 55 dogs. PROCEDURES Record databases 2 specialty practices were searched to identify conjunction furosemide for a minimum 28 days between January 2009 December 2015. Information extracted from records included signalment, tumor diagnosis, dosages, concurrent...

Ifosfamide (3‐[2‐chloroethyl]‐2[(2 chloroethyl)amino]tetrahydro‐2H‐1,3,2‐oxazaphosphorine 2‐oxide) is an alkylating agent with a broad spectrum of antitumor activity. The efficacy and toxicity ifosfamide were evaluated in 72 dogs spontaneously occurring tumors. Forty (56%) had lymphoma, 31 (43%) sarcomas, 1 metastatic carcinoma. Five received at dosages <350 mg/m 2 IV. Neither nor response observed, the remaining 350 (n = 18) 375 body surface area IV 49). Saline diuresis thiol compound...

Tasmanian Devil Facial Tumor Disease (DFTD) is a transmissible cancer threatening to cause the extinction of Devils in wild. The aim this study was determine susceptibility DFTD vincristine. Escalating dosage rates vincristine (0.05 0.136 mg/kg) were given devils early stages (n = 8). None these impacted outcome disease. A rate 0.105 mg/kg, significantly higher than that humans or domestic animals, found highest could be administered safely. Signs toxicity included anorexia, vomiting,...