A5064318684- Advanced biosensing and bioanalysis techniques
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Mycotoxins in Agriculture and Food
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Cervical Cancer and HPV Research
- Electrochemical Analysis and Applications
- Microbial infections and disease research
- Synthesis and Biological Evaluation
- Agricultural Productivity and Crop Improvement
- Plant and fungal interactions
- Antibiotics Pharmacokinetics and Efficacy
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Herpesvirus Infections and Treatments
- T-cell and Retrovirus Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Pharmacological Effects and Assays
Zhengzhou University
2016-2025
Peking University
2024
Ping An (China)
2024
Henan Science and Technology Department
2021-2022
Henan Academy of Sciences
2020
Jinling Institute of Technology
2020
Animal Science Research Institute
2008-2019
Heilongjiang Provincial Academy of Agricultural Sciences
2019
East China Normal University
2005-2008
African swine fever (ASF) is a devastating disease, which causing huge economic losses in China. Therefore, it urgent to provide rapid, highly specific and sensitive diagnostic method for the detection of virus (ASFV), ASF infectious agent. In this study, novel quantitative real-time polymerase chain reaction (qPCR) assay with lyophilized powder reagents (LPR), targeting major structural protein p72 gene, was established ASFV. This had many advantages, such as saving time money, good...
A controllable solid-state electrochemiluminescence (ECL) film based on efficient and stable quenching of ECL ruthenium(II) tris-(bipyridine) (Ru(bpy)32+) by oxidizing ferrocene (Fc) at the electrode is developed. The intensity correlated to distance which controlled conformation ferrocene-labeled DNA molecular beacon (Fc-MB) between Fc Ru(bpy)32+ immobilized electrode. adjustment conducted via complementary hybridizing with bases in loop Fc-MB changing temperature resultant double-stranded...
The CD2-like (CD2V) protein is a crucial antigen of African swine fever virus (ASFV). CD2V interacts with the cellular AP-1 protein, participates in intracellular transport virus, and induces neutralizing antibodies to partly protect from attack. In this study, specific dimeric was designed enhance antigenicity immunogenicity, expressed Bac-to-Bac baculovirus expression vector system purified by Ni-affinity chromatography. After animal immunization, five monoclonal (mAbs) (7E12, 22B3, 18A3,...
Hepatitis C virus (HCV) infection is a global public health threat. Reaching the World Health Organization’s objective for eliminating viral hepatitis by 2030 will require precise disease diagnosis. While immunoassays and qPCR play significant role in detecting HCV, rapid accurate point-of-care testing important pathogen identification. This study establishes reverse transcription recombinase-aided amplification–lateral flow dipstick (RT-RAA-LFD) assay to detect HCV. The intact workflow was...
African swine fever (ASF) is a viral disease caused by the virus that can be highly transmitted and lethal in domestic pigs. In absence of vaccine, effective diagnosis critical for minimizing virus’s spread. recent years, with decline (ASFV) virulence, antibody detection has become an important means detection. ASFV nucleocapsid protein p34 mature hydrolytic product pp220, which conserved high content structural virus. Prokaryotic cells were chosen to generate active high-yield protein, was...
ABSTRACT Human papillomavirus type 16 (HPV16) is a high-risk virus linked to cervical cancer, primarily through its oncogenic proteins E6 and E7. The HPV16-L1 protein, the major capsid component, plays key role in formation immune response. Monitoring anti-HPV16-L1 antibodies serum crucial for understanding infection dynamics vaccine efficacy. This study aimed develop novel quantum dot-labeled blocking enzyme-linked immunosorbent assay (QDs-B-ELISA) quantitative detection of antibodies....
To explore the relationship among level of fiber, gut microbiota, and nutritional substances, we applied next generation sequencing technology for identification composition structure microbiota in gastrointestinal tract. In this study, a total 25 phyla 298 genera were identified from tract; Firmicutes, Bacteroidetes, Proteobacteria predominant phyla. The ability cecum carbohydrate metabolism was significantly higher than that gizzard ileum (P < 0.05). bacterial community various stages...
To compensate for the seasonal imbalance between livestock and forage yield in cold region of Northeast China, alfalfa (Medicago sativa L.) continuous cropping has been widely employed animal husbandry. However, effects on soil properties, including physical, chemical biological are poorly understood. In this study, we investigated properties fungal community composition fields under different time periods (i.e., 1, 2, 6, 9, 12, 13 35 years). The results showed that moisture, total C, N,...