A5113427765- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Vector-Borne Animal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Cytomegalovirus and herpesvirus research
- Salmonella and Campylobacter epidemiology
- Veterinary medicine and infectious diseases
- Autophagy in Disease and Therapy
- Synthesis and biological activity
- vaccines and immunoinformatics approaches
- Plant Virus Research Studies
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Immune Response and Inflammation
Peking University
2022-2025
Henan Agricultural University
2016-2025
China Animal Disease Control Center
2025
Ministry of Education of the People's Republic of China
2025
Zhengzhou University
2020-2024
Henan Academy of Agricultural Sciences
2018-2024
Northwest A&F University
2024
Yangzhou University
2018-2022
Jilin University
2022
Henan Science and Technology Department
2020-2021
ABSTRACT African swine fever (ASF) is a highly infectious and lethal disease, leading to enormous losses in the pig industry. K205R, non-structural protein of ASF virus (ASFV), abundantly expressed at early stages viral infection induces strong immune response. In our previous study, five strains K205R-specific nanobodies (Nbs) were screened through phage display technology, among which Nb1, Nb14, Nb35, Nb82 exhibited good affinity. present above four Nbs successfully HEK293T cells...
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to the global spread disease 2019 (COVID-19), creating an urgent need for updated methods evaluate immune responses vaccines and therapeutic strategies. In this study, we introduce a novel cell-free, virus-free SARS-CoV-2 neutralizing antibody ELISA (NAb-ELISA), which is based on competitive inhibition receptor binding domain (RBD) spike protein angiotensin-converting enzyme (ACE2) receptor. method,...
Hepatitis E virus (HEV) infects both humans and animals, with an overall human mortality rate generally less than 1%, but as high 20% among pregnant women. HEV strains fall into 4 major genotypes. Zoonotic genotypes 3 associate sporadic animal cases in many industrialized countries. To date, collective evidence implicates pigs the main reservoir, justifying importance of monitoring infection rates pig herds to prevent illness. Due lack a robust vitro cell culture system for viral...
Abstract Classical swine fever (CSF) caused by the classical virus (CSFV) is a highly contagious disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and E rns are major targets for eliciting antibodies against CSFV infected animals. In this report, were expressed using baculovirus system their protective immunity rabbits tested. Twenty seronegative randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-E , or...
Abstract Background H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. AIV has a high mortality rate after infection humans, most human cases close contacted with poultry the live market. Therefore, it is necessary to develop rapid point-of-care testing (POCT) technique for detection. Methods The was inactivated purified, used as antigen immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced through...
The H9N2 avian influenza virus (AIV) remains a serious threat to the global poultry industry and public health. hemagglutinin (HA) protein is an essential protective antigen of AIVs major target neutralizing antibodies vaccines. Therefore, in this study, we used rice-derived HA as immunogen generate monoclonal (mAbs) screened them using immunoperoxidase monolayer assay indirect enzyme-linked immunosorbent assay. Eight mAbs reacted well with recombinant AIV protein, four which exhibited...
Multivalent presentation of antigens using nanoparticles (NPs) as a platform is an effective strategy to enhance the immunogenicity subunit vaccines and thus induce high level organismal immune response. Our previous results showed that pre-existing porcine circovirus type 2 (PCV2) antibodies could increase antibody levels nanoparticle carried in PCV2 VLPs. Here, we have established generalized nanoantigen display platform, Cap-Cat virus-like particles (VLPs). By combining VLPs with modular...
Viral infection causes endoplasmic reticulum stress and protein metabolism disorder, influencing aggregates formation or degradation that originate from misfolded proteins. The mechanism by which host proteins are involved in the above process remains largely unknown. present study found porcine reproductive respiratory syndrome virus (PRRSV) promoted of intracellular ubiquitinated via activating autophagy. cell E3 ligase tripartite motif-containing (TRIM)25 recruitment aggregation...
Influenza A virus (IAV), a deadly zoonotic pathogen, occasionally cross-species transmission among humans, swine and avian. The ectodomain of matrix protein 2 (M2e) is highly conserved in IAV has been widely concerned the development universal influenza vaccines. Due to low immunogenicity, multi-copy M2e are usually displayed on surface nanoparticles constitute nanovaccines. Here, we report that permutation affects immune effect nanovaccine. Three derived from avian were inserted into...
Influenza A virus (IAV), a deadly zoonotic pathogen, occasionally cross-species transmission among humans, swine and avian. The ectodomain of matrix protein 2 (M2e) is highly conserved in IAV, multi-copy M2e from different species are usually displayed on the surface nanoparticles to improve immunogenicity constitute universal IAV nanovaccines. In our previous study, three were inserted into C-terminal Cap porcine circovirus type (PCV2) form nanovaccine that protects PCV2 subtypes IAV....
Abstract Background: H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. AIV has a high mortality rate after infection humans, most human cases close contacted with poultry the live market. Therefore, it is necessary to develop rapid point-of-care testing (POCT) technique for detection. Methods: The was inactivated purified, used as antigen immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced...
Classical swine fever (CSF) is caused by classical virus (CSFV) and has led to huge ecnomic losses for the pig industry worldwide. Although vaccination other control measures have been carried out, it essential establish a rapid valid method CSF monitoring clinical diagnosis. CSFV E2 protein well-known as major antigen antibody detection. It significant improve affinity between better performance of detection method. In this study, recombinant extracellular (aa 1-331), which native homodimer...
Abstract Background: H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. AIV has a high mortality rate after infection humans, most human cases close contacted with poultry the live market. Therefore, it is necessary to develop rapid point-of-care testing (POCT) technique for detection. Methods: The was inactivated purified, used as antigen immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced...