A5014986772- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- interferon and immune responses
- Viral Infections and Vectors
- Immune Response and Inflammation
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Chemical synthesis and alkaloids
- Cancer-related molecular mechanisms research
- Alkaloids: synthesis and pharmacology
- Virology and Viral Diseases
Key Laboratory of Guangdong Province
2013-2024
South China Agricultural University
2015-2024
Ministry of Agriculture and Rural Affairs
2020-2024
Guangzhou Experimental Station
2022-2024
National Development and Reform Commission
2020-2022
South China Biological Medicine (China)
2020
National Influenza Center
2020
China Agricultural University
2020
National Institute for Public Health and the Environment
2016
Joint Research Center
2016
Abstract Human infections with avian influenza H7N9 or H10N8 viruses have been reported in China, raising concerns that they might cause human epidemics and pandemics. However, how these adapt to mammalian hosts is unclear. Here we show besides the commonly recognized viral polymerase subunit PB2 residue 627 K, other residues including 87E, 292 V, 340 588 648 V 676 M also play critical roles adaptation of virus. The avian-origin H10N8, H9N2 harboring PB2-588 exhibited higher activity, more...
ABSTRACT Since its emergence in 2013, the H7N9 low-pathogenic avian influenza virus (LPAIV) has been circulating domestic poultry China, causing five waves of human infections. A novel highly pathogenic (HPAIV) variant possessing multiple basic amino acids at cleavage site hemagglutinin (HA) protein was first reported two cases infection January 2017. More seriously, those HPAIV variants have transmitted and caused outbreaks on farms eight provinces China. Herein, we demonstrate presence...
Senecavirus A (SVA)-induced porcine idiopathic vesicular disease has caused huge economic losses worldwide. Glucose metabolism in the host cell is essential for SVA proliferation; however, impact of virus on glucose cells and subsequent effects are still unknown. Here, glycolysis induced by shown to facilitate replication promoting lactate production, which then attenuates interaction between mitochondrial antiviral-signaling protein (MAVS) retinoic acid-inducible gene I (RIG-I). induces...
Human infection with a novel influenza A(H10N8) virus was first described in China December 2013. However, the origin and genetic diversity of this is still poorly understood. We performed phylogenetic analysis coalescent two viruses from case (A/Jiangxi-Donghu/346-1/2013 A/Jiangxi-Donghu/346-2/2013 (A/chicken/Jiangxi/102/2013) isolated live poultry market that patient had visited. The haemagglutinin (HA), neuraminidase (NA), PA subunit polymerase complex, nucleoprotein (NP), M nonstructural...
To discover group-1-specific neuraminidase (NA) inhibitors that are especially involved in combating the H5N1 virus, two series of oseltamivir derivatives were designed and synthesized by targeting 150-cavity. Among these, compound 20l was most potent N1-selective inhibitor, with IC50 values 0.0019, 0.0038, 0.0067 μM against NAs from three viruses. These better than those carboxylate. Compound 32 another inhibitor exhibited a 12-fold increase activity H274Y mutant relative to Molecular...
Significance The emergence and transmission of avian influenza viruses (AIVs) pose a threat to public health result in enormous economic losses. Here we discover an association between the community structure poultry trade network AIV China by combining virus genomes statistical modeling trade. Importantly, are able “replicate” this finding comparing dynamics three strains (H5N1, H7N9, H5N6) that currently cocirculate China. Given detection continuous process geographic spread among poultry,...
H9N2 influenza viruses are globally endemic in birds, and a sharp increase human infections with occurred during 2021 to 2022. In this study, we assess the antigenic pathogenic impact of 23 hemagglutinin (HA) amino acid mutations. Our study reveals that three specific mutations, labeled R164Q, N166D, I220T, responsible for binding antibodies escape Variants containing R164Q I220T mutations viral replication avian mammalian cells. Furthermore, T150A found enhance mice, indicating these may...
The H10 subtype avian influenza virus (AIV) poses an ongoing threat to both birds and humans. Notably, fatal human cases of H10N3 H10N8 infections have drawn public attention. In 2022, we isolated two viruses (A/chicken/Shandong/0101/2022 A/chicken/Shandong/0603/2022) from diseased chickens in China. Genome analysis revealed that these were genetically associated with human-origin virus, internal genes originating local H9N2 viruses. Compared the (A/chicken/Jiangxi/102/2013), exhibited...
H9N2 subtype avian influenza viruses (AIVs) have shown expanded host range and can infect mammals, such as humans swine. To date the mechanisms of mammalian adaptation interspecies transmission AIVs remain poorly understood. explore molecular basis determining AIVs, we compared two field isolates in a mouse model: one (A/chicken/Guangdong/TS/2004, TS) is nonpathogenic, another (A/chicken/Guangdong/V/2008, V) lethal with efficient replication brains. In order to determine differences...
Both H9N2 avian influenza and 2009 pandemic H1N1 viruses (pH1N1) are able to infect humans swine, which has raised concerns that novel reassortant H9 with pH1N1 genes might be generated in these hosts by reassortment. Although previous studies have demonstrated show increased virulence mice transmissibility ferrets, the of natural such as chickens swine remain unknown. This study two (H9N2/CA09 H9N1/CA09) background A/California/04/2009 (CA09) virus replacing either both haemagglutinin (HA)...
The 2009 pandemic H1N1 virus (pH1N1) contains neuraminidase (NA) and matrix (M) genes from Eurasian avian-like swine influenza viruses (SIVs), with the remaining six North American triple-reassortant SIVs. To characterize role of pH1N1 NA M in pathogenesis transmission, their impact was evaluated background an (tr1930) SIV which HA (H3) (N2) A/swine/Texas/4199-2/98 were replaced those classical A/swine/Iowa/15/30 (1930) virus. laboratory-adapted 1930 did not shed nor transmit pigs, but...
ABSTRACT At least 10 different genotypes of novel reassortant H3N2 influenza viruses with 2009 pandemic H1N1 [A(H1N1)pdm09] gene(s) have been identified in U.S. pigs, including the variant a single A(H1N1)pdm09 M gene, which has infected more than 300 people. To date, only three these evaluated animal models, and pathogenicity transmissibility other seven genotype remain unknown. Here, we show that contain 3 (NP, M, NS) or 5 (PA, PB2, NP, genes from were pathogenic similar to endemic swine...
Abstract From 2013 onwards, the spread of novel H5N6 highly pathogenic avian influenza (HPAI) viruses in China has posed great threats to not only poultry industry but also human health. Since late-2016 particular, frequent outbreaks clade 2.3.4.4 HPAI among wild birds have promoted viral dissemination South Korea, Japan, and European countries. In response those trends, we conducted molecular genetic analysis global order characterize spatio-temporal patterns diffusion diversity poultry....
Abstract Low pathogenic avian influenza A(H9N2) virus is endemic worldwide and continually recruit internal genes to generate human-infecting H5N1, H5N6, H7N9, H10N8 variants. Here we show that hemagglutinin cleavage sites (HACS) of H9N2 viruses tended mutate towards hydrophilic via evolutionary transition, the tribasic HACS were found at high prevalence in Asia Middle East. Our finding suggested increased viral replication, stability, pathogenicity transmission chickens virulence mice...
Abstract Multiple recent outbreaks of highly pathogenic H5N8 viruses originating in aquatic birds frequently occurred most European countries, Russia, South Korea, and Japan during the winter 2020–21, one zoonotic event poultry workers infected with novel were reported Russia. Strikingly, these had emerged been co-circulating wild multiple provinces China 2020–21. In China, population has risen significantly past twenty years, is regarded as largest reservoir for influenza carried across...
While repeated infection of humans and enhanced replication transmission in mice has attracted more attention to it, the pathogenesis H9N2 virus was less known mice. PB2 residue 627 as virulent determinant H5N1 is associated with systemic impaired TCR activation, but impact this position on host immune response not been evaluated. In study, we quantified cellular mouse lung demonstrate that VK627 rTsE627K caused a significant reduction numbers T cells inflammatory (Macrophage, Neutrophils,...