A5030643460- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Connective tissue disorders research
- Collagen: Extraction and Characterization
- Ocular Surface and Contact Lens
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spaceflight effects on biology
- Advanced Glycation End Products research
- Optical Imaging and Spectroscopy Techniques
- Ocular Infections and Treatments
- Skin Protection and Aging
- Proteoglycans and glycosaminoglycans research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Angiogenesis and VEGF in Cancer
- Medical and Biological Ozone Research
- Optical Coherence Tomography Applications
- Protease and Inhibitor Mechanisms
- Glaucoma and retinal disorders
Harvard University
2017-2021
Massachusetts Eye and Ear Infirmary
2017-2021
Smith-Kettlewell Eye Research Institute
2017-2021
Chelsea Hospital
2019
University of Massachusetts Boston
2018
Cardiff University
2015-2017
Human corneal endothelial cells (HCEnCs) are terminally differentiated that have limited regenerative potential. The large numbers of mitochondria in HCEnCs critical for pump and barrier function required hydration transparency. Fuchs Endothelial Corneal Dystrophy (FECD) is a highly prevalent late-onset oxidative stress disorder characterized by progressive loss HCEnCs. We previously reported increased mitochondrial fragmentation reduced ATP mtDNA copy number FECD. Herein, carbonyl cyanide...
The cornea is the main refracting lens in eye. As part of outer tunic it has to be resilient, a property conferred by organisation constituent collagen. It also sufficiently elastic regain its exact shape when deformed, order not distort retinal image. basis this elasticity fully understood. purpose study was characterise three dimensions arrangement and distribution fibers human corneal stroma, using serial block face scanning electron microscopy. We have demonstrated that there exists...
The optical and biomechanical properties of the cornea are largely governed by collagen-rich stroma, a layer that represents approximately 90% total thickness. Within specific arrangement superimposed lamellae provides tissue with tensile strength, whilst spatial individual collagen fibrils within confers transparency. In keratoconus, this precise stromal is lost, resulting in ectasia visual impairment. normal cornea, we previously characterised three-dimensional an elastic fiber network...
The structural and optical properties of clinically biocompatible, cell-free hydrogels comprised synthetically cross-linked moulded recombinant human collagen type III (RHCIII) with without the incorporation 2-methacryloyloxyethyl phosphorylcholine (MPC) were assessed using transmission electron microscopy (TEM), X-ray scattering, spectroscopy refractometry. These findings examined alongside similarly obtained data from 21 donor corneas. TEM demonstrated presence loosely bundled aggregates...
Purpose: The presence of fibrillin-rich elastic fibers in the cornea has been overlooked recent years. aim current study was to elucidate their functional role using a mouse model for Marfan syndrome, defective fibrillin-1, major structural component microfibril bundles that constitute most fibers. Methods: Mouse corneas were obtained from animals with heterozygous fibrillin-1 mutation (Fbn1+/−) and compared wild type controls. Corneal thickness radius curvature calculated optical coherence...
The primary aim of this study was to quantify the relationship between corneal structure and hydration in humans pigs. X-ray scattering data were collected from human porcine corneas equilibrated with polyethylene glycol (PEG) varying levels hydration, obtain measurements collagen fibril diameter, interfibrillar spacing (IFS) intermolecular spacing. Both species showed a strong positive linear correlation IFS2 nonlinear, bi-phasic whereby diameter increased up approximately physiological H =...