A5063329916- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Acute Myeloid Leukemia Research
- RNA Research and Splicing
- Bone health and treatments
- HER2/EGFR in Cancer Research
- PI3K/AKT/mTOR signaling in cancer
- Hedgehog Signaling Pathway Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Kruppel-like factors research
- Viral Infections and Immunology Research
- Prostate Cancer Treatment and Research
- Mesenchymal stem cell research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Virus-based gene therapy research
- Topic Modeling
- Radiology practices and education
- Thyroid Cancer Diagnosis and Treatment
- Bluetooth and Wireless Communication Technologies
Stanford University
2020-2022
University of California, Davis
2017
University of California, San Diego
2006-2016
Sanford Consortium for Regenerative Medicine
2012-2016
Moores Cancer Center
2006-2012
Stem Cell Institute
2012
La Jolla Alcohol Research
2012
SummaryLeukemia stem cells (LSCs) play a pivotal role in the resistance of chronic myeloid leukemia (CML) to tyrosine kinase inhibitors (TKIs) and its progression blast crisis (BC), part, through alternative splicing self-renewal survival genes. To elucidate splice-isoform regulators human BC LSC maintenance, we performed whole-transcriptome RNA sequencing, splice-isoform-specific quantitative RT-PCR (qRT-PCR), nanoproteomics, stromal coculture, xenotransplantation analyses. Cumulatively,...
The molecular etiology of human progenitor reprogramming into self-renewing leukemia stem cells (LSC) has remained elusive. Although DNA sequencing uncovered spliceosome gene mutations that promote alternative splicing and portend leukemic transformation, isoform diversity also may be generated by RNA editing mediated adenosine deaminase acting on (ADAR) enzymes regulate cell maintenance. In this study, whole-transcriptome normal, chronic phase, serially transplantable blast crisis myeloid...
Dormant leukemia stem cells (LSC) promote therapeutic resistance and leukemic progression as a result of unbridled activation cell gene expression programs. Thus, we hypothesized that 1) deregulation the hedgehog (Hh) self-renewal cycle regulatory pathway would dormant human LSC generation 2) PF-04449913, clinical antagonist GLI2 transcriptional activator, smoothened (SMO), enhance eradication. To test these postulates, whole transcriptome RNA sequencing (RNA-seq), microarray, qRT-PCR,...
Background Leukemia initiating cells (LIC) contribute to therapeutic resistance through acquisition of mutations in signaling pathways, such as NOTCH1, that promote self-renewal and survival within supportive niches. Activating NOTCH1 occur commonly T cell acute lymphoblastic leukemia (T-ALL) have been implicated resistance. However, the type context specific consequences activation, its role human LIC regeneration, sensitivity inhibition hematopoietic microenvironments had not elucidated....
Abstract Prostate cancer metastasizes to bone in the majority of patients with advanced disease leading painfully debilitating fractures, spinal compression and rapid decline. In addition, prostate metastases often become resistant standard therapies including androgen deprivation, radiation chemotherapy. There are currently few models elucidate mechanisms interaction between microenvironment cancer. It is, thus, essential develop new patient-derived, orthotopic models. Here we report...
To automatically identify a cohort of patients with pancreatic cystic lesions (PCLs) and extract PCL measurements from historical CT MRI reports using natural language processing (NLP) question answering system.Institutional review board approval was obtained for this retrospective Health Insurance Portability Accountability Act-compliant study, the requirement to obtain informed consent waived. A free-text generated between January 1991 July 2019 that covered region were identified....
To develop a deep learning-based risk stratification system for thyroid nodules using US cine images.In this retrospective study, 192 biopsy-confirmed (175 benign, 17 malignant) in 167 unique patients (mean age, 56 years ± 16 [SD], 137 women) undergoing between April 2017 and May 2018 with American College of Radiology (ACR) Thyroid Imaging Reporting Data System (TI-RADS)-structured radiology reports were evaluated. A that exploits the images obtained during three-dimensional volumetric...
In Ph-positive (Ph(+)) leukemia, the quiescent cell state is one of reasons for resistance to BCR-ABL-kinase inhibitor, imatinib. order examine mechanisms due quiescence and effect mammalian target rapamycin everolimus, such a resistant population, we used Ph(+) acute lymphoblastic leukemia patient cells serially xenotransplanted into NOD/SCID/IL2rγ(null) (NOG) mice. Spleen from leukemic mice showed higher percentage slow-cycling G(0) in CD34(+)CD38(-) population compared with CD34(+)CD38(+)...
The only therapeutic options that exist for squamous cell lung carcinoma (SCC) are standard radiation and cytotoxic chemotherapy. Cancer stem cells (CSCs) hypothesized to account resistance, suggesting CSCs must be specifically targeted. Here, we analyze the transcriptome of CSC non-CSC subpopulations by RNA-seq identify new potential strategies SCC.We sorted a SCC into CD133- CD133+ then examined both copy number analysis (CNA) whole genome sequencing. We analyzed Genome Atlas (TCGA) data...
Abstract The molecular etiology of human progenitor reprogramming into self-renewing leukemia stem cells (LSC) has remained elusive. While DNA sequencing uncovered spliceosome gene mutations that promote alternative splicing and portend leukemic transformation, isoform diversity may also be generated by RNA editing mediated adenosine deaminase acting on (ADAR) enzymes regulate cell maintenance. In this study, whole transcriptome normal, chronic phase (CP) serially transplantable blast crisis...