A5072079412- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Adipokines, Inflammation, and Metabolic Diseases
- Gut microbiota and health
- Adrenal Hormones and Disorders
- Diabetes Treatment and Management
- Vitamin C and Antioxidants Research
- Immune cells in cancer
- Metabolism, Diabetes, and Cancer
- Adenosine and Purinergic Signaling
- Renal Transplantation Outcomes and Treatments
- Diet and metabolism studies
- Digestive system and related health
- T-cell and B-cell Immunology
- COVID-19 Impact on Reproduction
- Corneal Surgery and Treatments
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Xenotransplantation and immune response
- Dietary Effects on Health
- Nutritional Studies and Diet
- Clostridium difficile and Clostridium perfringens research
Université Libre de Bruxelles
2021-2023
Universidade de São Paulo
2012-2019
KU Leuven
2009-2016
Catholic University of America
2014
Current interventions for arresting autoimmune diabetes have yet to strike the balance between sufficient efficacy, minimal side effects, and lack of generalized immunosuppression. Introduction antigen via gut represents an appealing method induction antigen-specific tolerance. Here, we developed a strategy tolerance restoration using mucosal delivery in mice biologically contained Lactococcus lactis genetically modified secrete whole proinsulin autoantigen along with immunomodulatory...
The vitamin D receptor (VDR) is a hormone nuclear regulating bone and calcium homeostasis. Studies revealing the expression of VDR on immune cells point toward role for VDR-dependent signaling pathways in immunity. Here we verified ability natural ligand, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to interfere inflammatory T cell stimulatory capacity macrophages, particular within chronic disease features experimental type 1 diabetes (T1D). We demonstrated that constitutively expressed...
Growing insight into the pathogenesis of type 1 diabetes (T1D) and numerous studies in preclinical models highlight potential antigen-specific approaches to restore tolerance efficiently safely. Oral administration protein antigens is a preferred method for induction, but degradation during gastrointestinal passage can impede such protein-based therapies, reducing their efficacy making them cost-ineffective. To overcome these limitations, we generated tolerogenic bacterial delivery...
High doses of the active form vitamin D3, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], prevent diabetes in NOD mouse but also elicit unwanted calcemic side effects. Because immune cells themselves can convert into 1,25(OH)2D3 locally, we hypothesized that dietary disease. Thus, evaluated whether administration high regular (800 IU/day) during different periods life (pregnancy and lactation, early [3-14 weeks age], or lifelong [3-35 age]) safely prevents mice. We found only treatment raised serum...
The introduction of β-cell autoantigens via the gut through Lactococcus lactis (L. lactis) has been demonstrated to be a promising approach for diabetes reversal in NOD mice. Here we show that combination therapy low-dose anti-CD3 with clinical-grade self-containing L. lactis, appropriate human application, secreting proinsulin and interleukin-10, cured 66% mice new-onset diabetes, which is comparable results plasmid-driven lactis. Initial blood glucose concentrations (<350 mg/dL)...
The short-chain fatty acids (SCFAs) are metabolites originated from the fermentation of dietary fibers and amino produced by bacteria intestinal microbiota. most abundant SCFAs, acetate, propionate, butyrate, have been proposed as a treatment for inflammatory bowel diseases (IBDs) due to their anti-inflammatory properties. This work aimed analyze effects three combined SCFAs in TNBS-induced inflammation zebrafish larvae. Here, we demonstrated that significantly increased survival...
Combining immune intervention with therapies that directly influence the functional state of β-cells is an interesting strategy in type 1 diabetes cure. Dipeptidyl peptidase-4 (DPP-4) inhibitors elevate circulating levels active incretins, which have been reported to enhance insulin secretion and synthesis, can support β-cell survival possibly stimulate proliferation neogenesis. In current study, we demonstrate DPP-4 inhibitor MK626, has appropriate pharmacokinetics mice, preceded by a...
Little is known about the diversity in immune profile of different wild type strains zebrafish (Danio rerio), despite its growing popularity as an animal model to study human diseases and drug testing. In case data resulting from modeling diseases, differences background Danio fishes have rarely been taken into consideration when interpreting results this potentially problematic, many studies not even mention source strain animals. study, we hypothesized that could present distinct traits....
Type 1 diabetes (T1D) is caused by progressive immune-mediated loss of insulin-producing β-cells. Inflammation detrimental to β-cell function and survival, moreover, both apoptosis necrosis have been implicated as mechanisms in T1D. The receptor interacting serine/threonine protein kinase (RIPK1) promotes inflammation serving a scaffold for NF-κB MAPK activation, or acting that triggers necroptosis. It unclear whether RIPK1 activity involved T1D pathology. In the present study, we...
The transcription factor nuclear factor-κB (NF-κB) has a key role in the pathogenesis of diabetes and its complications. Although activation canonical NF-κB pathway β-cells is generally deleterious, little known about non-canonical signalling main regulator, NF-κB-inducing kinase (NIK), on pancreatic β-cell survival function. Previous studies based models NIK overexpression islet cells showed that induced either spontaneous death due to inflammation or glucose intolerance during diet-induced...
We performed a comparative study and evaluated cellular infiltrates anti-inflammatory cytokine production at different time-points after syngeneic or allogeneic skin transplantation. observed an early IL-10 in grafts compared with allografts. This observation prompted us to investigate the role of isograft acceptance. For this, we used KO WT mice perform transplantation, where was absent graft recipient. The majority derived from donors did not engraft only partially accepted, whereas...
Organ transplantation is a life-saving procedure, however predicting graft survival still challenging.Understanding immune-cell pathobiology critical to the development of effective therapies prevent rejection.Over recent years it has become progressively evident that complex nature immune cell behavioral dynamics strongly dependent on cellular metabolism, which in turn, relies competition for nutrients, oxygen and metabolites with other cells microbiota.Furthermore, influence inflammatory...