A5054194316- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Environmental Toxicology and Ecotoxicology
- Bacteriophages and microbial interactions
- Marine and coastal ecosystems
- Mercury impact and mitigation studies
- Marine Bivalve and Aquaculture Studies
- Coral and Marine Ecosystems Studies
- Antibiotic Resistance in Bacteria
- Marine and coastal plant biology
- Aquaculture disease management and microbiota
- Protist diversity and phylogeny
- Vibrio bacteria research studies
- Glutathione Transferases and Polymorphisms
- Cholinesterase and Neurodegenerative Diseases
- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Drug Transport and Resistance Mechanisms
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine animal studies overview
- Methane Hydrates and Related Phenomena
- Healthcare cost, quality, practices
- Bacterial biofilms and quorum sensing
- Marine Toxins and Detection Methods
- Innovative Microfluidic and Catalytic Techniques Innovation
Haverford College
2018-2025
Tulane University
2019
Woods Hole Oceanographic Institution
2007-2016
University of Virginia Health System
2014
Scripps Institution of Oceanography
2010-2012
University of California, San Diego
2010
College of Charleston
2009
Boise State University
2009
UNSW Sydney
2009
University of California, Santa Barbara
2009
Significance The majority of pharmaceuticals are inspired by natural product scaffolds that functionalized tailoring enzymes, such as halogenases. degree halogenation is an important determinant bioactivity, yet little known regarding the molecular basis for exquisite control exhibited Known pyrrole halogenases commonly perform up to two halogenations on pyrrole. Our study tetrabromopyrrole biosynthesis revealed a uniquely adapted halogenase–thioesterase enzyme pair catalyzes unprecedented...
Acute kidney injury (AKI) is a common problem in hospitalized patients that enhances morbidity and mortality promotes the development of chronic end-stage renal disease. Ischemia reperfusion (IRI) one major causes AKI characterized by uncontrolled inflammation tubular epithelial cell death. Our recent studies demonstrated regulatory T cells (Tregs) protect from ischemia reperfusion-induced injury. Blockade programmed death-1 (PD-1) on surface Tregs, prior to adoptive transfer, negates their...
Members of the resistance nodulation cell division (RND) efflux pumps play essential roles in multidrug (MDR) Gram-negative bacteria. Here, we describe search for new small molecules from marine microbial extracts to block and thus restore antibiotic susceptibility MDR bacterial strains. We report isolation 3,4-dibromopyrrole-2,5-dione (1), an inhibitor RND transporters, Enterobacteriaceae Pseudomonas aeruginosa, bacterium Pseudoalteromonas piscicida. 3,4-Dibromopyrrole-2,5-dione decreased...
Interactions between phytoplankton and bacteria play a central role in mediating biogeochemical cycling food web structure the ocean. However, deciphering chemical drivers of these interspecies interactions remains challenging. Here, we report isolation 2-heptyl-4-quinolone (HHQ), released by Pseudoalteromonas piscicida, marine gamma-proteobacteria previously reported to induce mortality through hitherto unknown algicidal mechanism. HHQ functions as both an antibiotic bacterial signaling...
Within our lakes, streams, estuaries, and oceans, there is an astounding chemodiversity of secondary metabolites produced by microbes, algae, invertebrates. Nearly 30 years study have yielded hundreds examples in which alter the foraging behavior or fitness aquatic consumers, both. However, understanding mechanisms that mediate fate consequences these consumers remains its infancy. Interactions between at molecular biochemical level are purview modern pharmacology, rooted long history...
Bacteria and phytoplankton form close associations in the ocean that are driven by exchange of chemical compounds. The bacterial signal 2-heptyl-4-quinolone (HHQ) slows growth; however, mechanism responsible remains unknown.
Abstract Eukaryotic phytoplankton contribute to the flow of elements through marine food webs, biogeochemical cycles, and Earth’s climate. Therefore, how die is a critical determinate fate nutrients. While heterotroph grazing viral infection mortality, recent evidence suggests that bacteria-derived cues also control lysis. Here, we report exposure nanomolar concentrations 2,3,4,5-tetrabromopyrrole (TBP), brominated chemical cue synthesized by γ-proteobacteria, resulted in mortality seven...
Marine bacteria form complex relationships with eukaryotic hosts, from obligate symbioses to pathogenic interactions. These interactions can be tightly regulated by bioactive molecules, creating a system of chemical through which these species chemically communicate thereby directly altering the host's physiology and community composition. Quorum sensing (QS) signals were first described in marine bacterium four decades ago, since then, we have come discover that QS mediates processes within...
Abstract Background Intense consumer pressure strongly affects the structural organization and function of marine ecosystems, while also having a profound effect on phenotype both predator prey. Allelochemicals produced by prey often render their tissues unpalatable or toxic to majority potential consumers, yet some consumers have evolved resistance host chemical defenses. A key challenge facing ecologists seeking explain vast differences in tolerance dietary allelochemicals is understanding...
Biofilm-forming bacteria have the potential to contribute health, physiology, behavior and ecology of host serve as its first line defense against adverse conditions in environment. While metabarcoding metagenomic information furthers our understanding microbiome composition, fewer studies use cultured samples study diverse interactions among microbiome, representatives are often lacking. This examines surface microbiomes from three shallow-water coral species two whale species. These unique...
The emergence of multi-drug resistant pathogenic bacteria represents a serious and growing threat to national healthcare systems. Most pressing is an immediate need for the development novel antibacterial agents treat Gram-negative infections, including opportunistic, hospital-derived pathogen, Acinetobacter baumannii. Herein we report naturally occurring 1,2-benzisoxazole with minimum inhibitory concentrations as low 6.25 μg ml
Background Despite the profound variation among marine consumers in tolerance for allelochemically-rich foods, few studies have examined biochemical adaptations underlying diet choice. Here we examine role of glutathione S-transferases (GSTs) detoxification dietary allelochemicals digestive gland predatory gastropod Cyphoma gibbosum, a generalist consumer gorgonian corals. Controlled laboratory feeding experiments were used to investigate influence on GST activity and isoform expression....
Fertilization changes the structure and function of cell surface. In sea urchins, these include polymerization cortical actin a coincident, switch-like increase in activity multidrug efflux transporter ABCB1a. However, it is not clear how reorganization leads to membrane transport physiology. this study, we used three-dimensional superresolution fluorescence microscopy resolve fine-scale movements along polymerizing filaments, show that established after ABCB1a translocates tips microvilli....
Synopsis The cumulative outcome of bacteria-phytoplankton cell-cell interactions has global-scale consequences that necessitate a more comprehensive understanding the species form these relationships, chemical exchanges govern them, and cues trigger them. However, diffuse liquid environment supporting is inherently difficult to interrogate, which moved researchers combine multi-omics analyses, genome mining tools, genetic probes, mathematical models gain insight into networks existing around...
In disciplines like toxicology and pharmacology, oxygen (O 2 ) respiration is a universal metric for evaluating the effects of chemicals across various model systems, including mammalian microalgal cells.
Interactions between marine phytoplankton, viruses, and bacteria drive biogeochemical cycling, shape trophic structures, impact global climate. Microbially produced compounds have emerged as key players in influencing eukaryotic organismal physiology, turn, remodel microbial community structure. This work aimed to reveal the molecular mechanism by which bacterial quorum sensing molecule 2-heptyl-4-quinolone (HHQ), gammaproteobacterium Pseudoalteromonas spp., arrests cell division confers...
With the lack of new antibiotics in drug discovery pipeline, coupled with accelerated evolution antibiotic resistance, sources that target pathogens clinical importance are paramount. Here, we use bacterial cytological profiling to identify mechanism action monounsaturated fatty acid (