A5046819570- Biochemical effects in animals
- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Chemical Analysis and Environmental Impact
- Alcohol Consumption and Health Effects
- Spectroscopy and Chemometric Analyses
- Vitamin C and Antioxidants Research
- Autism Spectrum Disorder Research
- Planetary Science and Exploration
- Nerve injury and regeneration
- bioluminescence and chemiluminescence research
- Polyamine Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- Food Drying and Modeling
- Metabolomics and Mass Spectrometry Studies
- Drug-Induced Hepatotoxicity and Protection
- Carcinogens and Genotoxicity Assessment
- Biocrusts and Microbial Ecology
- Geochemistry and Elemental Analysis
- Glaucoma and retinal disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Meat and Animal Product Quality
- Sulfur Compounds in Biology
- Edible Oils Quality and Analysis
- Building Energy and Comfort Optimization
University of Patras
2010-2022
The combination of intense solar radiation and soil desiccation creates a short circuit in the biogeochemical carbon cycle, where soils release significant amounts CO2 reactive nitrogen oxides by abiotic oxidation. Here we show that desert accumulate metal superoxides peroxides at higher levels than non-desert soils. We also photogeneration equimolar superoxide hydroxyl radical desiccated aqueous soils, respectively, photo-induced electron transfer mechanism supported their mineralogical...
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by carbonyl-DNPH hydrazone formation at acidic pH presence denaturing urea, and subsequent solubilization SDS stabilization acid hydrolysis 7.0. At this neutral (ntr) pH, interfering unreacted DNPH uncharged its thus increased hydrophobicity permits 100% effective removal solubilizate with ethyl acetate/hexane wash. The ntrDNPH more...
Background. Intestinal mucosal barrier dysfunction in liver cirrhosis and its implicated mechanisms is of great clinical importance because it associated with the development serious complications from diverse organs through promotion systemic endotoxemia. Aim. The present study was designed to investigate whether enterocytes’ proliferation, apoptosis intestinal oxidative stress are altered mucosa patients compensated decompensated cirrhosis. Material methods. Twelve healthy controls (group...
The present study demonstrates that γ-radiolyzed perchlorate-containing Mars soil salt analogues (in a CO2 atmosphere) generate upon H2O wetting the reactive oxygen species (ROS) superoxide radical (O2•−), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). This also validates analogue radiolysis forms oxychlorine that, in turn, can UV-photolyze to •OH UV photolysis. investigation was made possible by development of new assay for inorganic-origin O2•− H2O2 determination modification...
A new fluorometric assay is presented for the ultrasensitive quantification of total protein carbonyls, and based on their specific reaction with rhodamine B hydrazide (RBH), production a carbonyl-RBH hydrazone fluorescence which (at ex/em 560/585 nm) greatly enhanced by guanidine-HCl. Compared to fluorescein-5-thiosemicarbazide (FTC)-based assay, RBH uses 24-fold shorter incubation time (1 h) at least 1000-fold lower quantity (2.5 µg), produces very reliable data that were verified...
This study presents an assay for the detection and quantification of soil metal superoxides peroxides in regolith soil. The O2 release (OR) is based on enzymatic conversion hydrolysis products oxides to their by electrode stoichiometry involved reactions. intermediate product O₂˙⁻ from converted cytochrome c superoxide dismutase (SOD) ½ mol H2O2, which then catalase (CAT) O2. H2O2 hydroperoxides CAT. method was validated a sealed sample chamber using liquid-phase Clark-type with known...
Objectives: This work, on low-cost controlled freeze methodology for stabilizing the phenolic content in Extra Virgin Olive Oil (EVOO), is evaluated by studying four Quality Indexes (QIs): free acidity, as % of oleic acid (1st-QI); UV-absorption values; K272, K232 and ΔK (2nd-QI); total (TPC), Gallic equivalence (3rd-QI); lipid peroxidation products, malondialdehyde (MDA) (4th-QI). Methods: The study QIs EVOO under storage, absence light, at 25, 4, -20 -80°C, with or without N2-purge showed...
Obstructive jaundice induces oxidative changes in the brain parenchyma and plays significant role clinical manifestations of hepatic encephalopathy. We aim to study progression status over time differences its pattern hemispheres, brainstem cerebellum. use an experimental model rats measuring stress (OS) specific biomarkers protein malondialdehyde (PrMDA) carbonyls (PrC = O). Hyperbilirubinemia has been confirmed all groups as result common bile duct obstruction. increase both PrMDA PrC O...