A5011623107- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- DNA and Nucleic Acid Chemistry
- Conferences and Exhibitions Management
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Ionosphere and magnetosphere dynamics
- Sport and Mega-Event Impacts
- Geophysics and Gravity Measurements
- Mycobacterium research and diagnosis
- Cultural Industries and Urban Development
- Biosensors and Analytical Detection
- Leptospirosis research and findings
- Photosynthetic Processes and Mechanisms
- Cancer therapeutics and mechanisms
- Educational Games and Gamification
- Team Dynamics and Performance
- Parasitic Infections and Diagnostics
Polar Scientific
2023-2024
Centre Interdisciplinaire de Recherche en Biologie
2017-2024
Centre National de la Recherche Scientifique
2017-2023
Institut Pasteur
2020-2023
Université Paris Cité
2022-2023
Université Paris Sciences et Lettres
2017-2022
Collège de France
2017-2022
Inserm
2017-2022
Structure et Instabilité des Génomes
2020-2021
Department of Genomes & Genetics
2020-2021
Here, we report the design, construction, and characterization of a tRNA neochromosome, designer chromosome that functions as an additional, de novo counterpart to native complement Saccharomyces cerevisiae. Intending address one central design principles Sc2.0 project, ∼190-kb neochromosome houses all 275 relocated nuclear genes. To maximize stability, incorporates orthogonal genetic elements from non-S. cerevisiae yeast species. Furthermore, presence 283 rox recombination sites enables...
Abstract Transcription generates local topological and mechanical constraints on the DNA fiber, leading to generation of supercoiled chromosome domains in bacteria. However, global impact transcription organization remains elusive, as scale genes operons bacteria well below resolution chromosomal contact maps generated using Hi-C (~5–10 kb). Here we combined sub-kb engineering visualize individual transcriptional units. We show that units form discrete three-dimensional transcription-induced...
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces an antibody response targeting multiple antigens that changes over time. This study aims to take advantage of this complexity develop more accurate serological diagnostics.A multiplex assay was developed measure IgG and IgM responses seven SARS-CoV-2 spike or nucleoprotein antigens, two for the nucleoproteins 229E NL63 seasonal coronaviruses, three non-coronavirus antigens. Antibodies were measured in serum...
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time.
Homologous recombination provides a mechanism of DNA double-strand break repair (DSBR) that requires an intact, homologous template for synthesis. When synthesis associated with DSBR is convergent, the broken strands are replaced and accurate. However, if divergent established, over-replication flanking may occur deleterious consequences. The RecG protein Escherichia coli helicase translocase can re-model 3-way 4-way structures such as replication forks Holliday junctions. primary role in...
Significance Maintaining genomic integrity is crucial for cell survival. In Escherichia coli , RecA-mediated homologous recombination plays an essential role in the repair of DNA double-strand breaks (DSBs). A greater understanding mechanism requires quantitative analysis studies live cells. We have developed a novel method that able to capture these interactions on genome-wide scale by combining ChIP-seq and mathematical modeling interpret patterns RecA–DNA interaction during DSB (DSBR)....
Abstract In bacteria, chromosome segregation occurs progressively from the origin to terminus within minutes of replication each locus. Between and segregation, sister loci are held in an apparent cohesive state by topological links. The decatenation activity topoisomerase IV (Topo IV) is required for replicated loci, yet little known about structuring maintained a state. this work, we investigated folding cells with altered activities. Within after Topo inactivation, massive reorganization...
Abstract Aberrant DNA replication is a major source of the mutations and chromosomal rearrangements associated with pathological disorders. In bacteria, several different lesions are repaired by homologous recombination, process that involves sister chromatid pairing. Previous work in Escherichia coli has demonstrated interactions (SCIs) mediated topological links termed precatenanes, controlled topoisomerase IV. present work, we demonstrate during repair mitomycin C-induced lesions, rapidly...
Abstract Background Infection with SARS-CoV-2 induces an antibody response targeting multiple antigens that changes over time. This complexity presents challenges and opportunities for serological diagnostics. Methods A multiplex assay was developed to measure IgG IgM responses seven spike or nucleoprotein antigens, two the nucleoproteins of 229E NL63 seasonal coronaviruses, three non-coronavirus antigens. Antibodies were measured in serum samples from patients French hospitals RT-qPCR...
In bacteria, DNA methylation can be facilitated by 'orphan' methyltransferases lacking cognate restriction endonucleases, but whether and how these enzymes control key cellular processes are poorly understood. The effects of a specific modification, 4-methylcytosine (4mC), even less clear, as this epigenetic marker is unique to bacteria archaea, whereas the bulk research currently performed on eukaryotes. Here, we characterize 4mC methyltransferase from understudied pathogen Leptospira spp....
Abstract Here we report the design, construction and characterization of a tRNA neochromosome, designer chromosome that functions as an additional, de novo counterpart to native complement Saccharomyces cerevisiae . Intending address one central design principles Sc2.0 project, ∼190 kb neochromosome houses all 275 relocated nuclear genes. To maximize stability, incorporated orthogonal genetic elements from non- S. yeast species. Furthermore, presence 283 rox recombination sites enable...
Abstract Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time. Waning levels lead to reduced sensitivity serological diagnostic tests This undermines the utility surveillance as SARS-CoV-2 pandemic progresses into its second year. Here we develop multiplex test for measuring antibodies three isotypes (IgG, IgM, IgA) five antigens (Spike (S), receptor binding domain...
Chromosome conformation capture (Hi-C) has become a routine method for probing the 3D organization of genomes. However, when applied to bacteria and archaea, current protocols are expensive limited in their resolution. By dissecting different steps published eukaryotic prokaryotic Hi-C protocols, we have developed cost- time-effective approach generate high-resolution (down 500 bp – 1 kb) contact matrices both archaea For complete details on use execution this protocol, please refer Cockram...
Transcription generates local topological and mechanical constraints along the DNA fiber, driving for instance generation of supercoiled chromosomal domains in bacteria. However, global impact transcription-based regulation chromosome organization remains elusive. Notably, scale genes operons bacteria well below resolution contact maps generated using Hi-C (~ 5 – 10 kb), preventing to resolve transcription on genomic at fine-scale. Here, we combined sub-kb engineering visualize individual...
Abstract. The past decade has seen a global transformation in how we communicate and connect with one another, making it easier to network collaborate colleagues worldwide. COVID-19 pandemic led rapid unplanned shift toward virtual platforms, resulting several accessibility challenges that have excluded many people during events. Virtual hybrid conferences the potential present opportunities collaborations groups previously from purely in-person conference formats. This can only be achieved...
Accurate repair of DNA double-strand breaks (DSBs) is crucial for cell survival and genome integrity. In Escherichia coli , DSBs are repaired by homologous recombination (HR), using an undamaged sister chromosome as template. The intermediates this pathway expected to be branched molecules that may include 4-way structures termed Holliday junctions (HJs), 3-way such D-loops forks. Using a tool creating site-specific, repairable DSB on only one pair replicating chromosomes, we have determined...
Over the last decade, way we communicate and engage with one another has changed on a global scale. It is now easier than ever to network collaborate colleagues worldwide. But, COVID-19 pandemic led rapid unplanned move virtual platforms, resulting in several accessibility challenges inadvertent exclusion of people during online events. While virtual/hybrid events have strong potential facilitate new opportunities networks for everyone, they are also greatly positioned increase inclusion...