A5078626534- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Cancer therapeutics and mechanisms
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Acute Myeloid Leukemia Research
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Microtubule and mitosis dynamics
- Protein Degradation and Inhibitors
- Chromosomal and Genetic Variations
- DNA and Nucleic Acid Chemistry
- DNA and Biological Computing
- Immune Response and Inflammation
- bioluminescence and chemiluminescence research
- Diffusion and Search Dynamics
- Viral gastroenteritis research and epidemiology
- Protist diversity and phylogeny
- Synthetic Organic Chemistry Methods
- Bacterial biofilms and quorum sensing
- Immunotherapy and Immune Responses
- Molecular Communication and Nanonetworks
Collège de France
2016-2025
Centre Interdisciplinaire de Recherche en Biologie
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Inserm
2015-2024
Université Paris Sciences et Lettres
2017-2024
Université Paris 1 Panthéon-Sorbonne
2018
Universitat Pompeu Fabra
2013
Université Paris-Sud
2012
Centre de Génétique Moléculaire
2001-2012
Memorial Sloan Kettering Cancer Center
2003-2004
Abstract Transcription generates local topological and mechanical constraints on the DNA fiber, leading to generation of supercoiled chromosome domains in bacteria. However, global impact transcription organization remains elusive, as scale genes operons bacteria well below resolution chromosomal contact maps generated using Hi-C (~5–10 kb). Here we combined sub-kb engineering visualize individual transcriptional units. We show that units form discrete three-dimensional transcription-induced...
The organization of the Escherichia coli chromosome has been defined genetically as consisting four insulated macrodomains and two less constrained regions. Here we have examined movement chromosomal loci by tracking fluorescent markers in time-lapse microscopy during a complete cell cycle. Analysing positioning, segregation pattern motility allowed us to show that dynamic behaviour belonging various regions is radically different. In constraints on mobility are apparent whereas...
Chromosome organizations of related bacterial genera are well conserved despite a very long divergence period. We have assessed the forces limiting genome plasticity in Escherichia coli by measuring respective effect altering different parameters, including DNA replication, compositional skew replichores, coordination gene expression with replication-associated dosage, and chromosome organization into macrodomains. Chromosomes were rearranged large inversions. Changes replication or dosage...
Article16 July 2018Open Access Source DataTransparent process Characterizing meiotic chromosomes' structure and pairing using a designer sequence optimized for Hi-C Héloïse Muller Department Genomes Genetics, Groupe Régulation Spatiale des Génomes, Institut Pasteur, Paris, France CNRS, UMR 3525, Center of Bioinformatics, Biostatistics Integrative Biology (C3BI), Search more papers by this author Vittore F Scolari Nicolas Agier Laboratory Computational Quantitative Biology, de Biologie...
Abstract Acute myeloid leukemia (AML) pathogenesis often involves a mutation in the NPM1 nucleolar chaperone, but bases for its transforming properties and overall association with favorable therapeutic responses remain incompletely understood. Here we demonstrate that an oncogenic mutant form of (NPM1c) impairs mitochondrial function. NPM1c also hampers formation promyelocytic (PML) nuclear bodies (NB), which are regulators fitness key senescence effectors. Actinomycin D (ActD), antibiotic...
FtsK and topoisomerase (Topo) IV are both involved in chromosome segregation Escherichia coli. The former protein resides at the septal ring is required for resolution of dimers. latter chromosomal decatenase. We have demonstrated recently that Topo activity concentrated proximal regions nucleoids late cell cycle. Here we demonstrate physically functionally interact. was recovered immunoprecipitates FtsK. Two-hybrid analysis immunoblotting showed this interaction mediated by ParC subunit IV....
The mechanisms that control chromosome conformation and segregation in bacteria have not yet been elucidated. In Escherichia coli, the mere presence of an active process remains open question. Here, we investigate pattern E. coli genome by performing numerical simulations on a polymer model chromosome. We analyze roles intrinsic structuring chromosomes forced localization specific loci, which are observed vivo. Specifically, examine is divided into four structured macrodomains (MDs) two...
The organization of the Escherichia coli chromosome into a ring composed four macrodomains and two less-structured regions influences segregation sister chromatids mobility chromosomal DNA. structuring terminus region (Ter) macrodomain relies on interaction protein MatP with 13-bp target called matS repeated 23 times in 800-kb-long domain. Here, by using new method that allows transposition any segment at defined position genetic map, we reveal site-specific system restricts to Ter...
Catenation links between sister chromatids are formed progressively during DNA replication and involved in the establishment of chromatid cohesion. Topo IV is a bacterial type II topoisomerase removal catenation both behind forks after final separation chromosomes. We have investigated global DNA-binding catalytic activity E. coli using genomic molecular biology approaches. ChIP-seq revealed that interaction with chromosome controlled by replication. During replication, has access to most...
Abstract Patients with Crohn’s disease exhibit abnormal colonization of the intestine by adherent invasive E. coli (AIEC). They adhere to epithelial cells, colonize them and survive inside macrophages. It appeared recently that AIEC LF82 adaptation phagolysosomal stress involves a long lag phase in which many cells become antibiotic tolerant. Later during infection, they proliferate vacuoles form colonies harboring dozens bacteria. In present work, we investigated mechanism sustaining this...
Abstract In bacteria, chromosome segregation occurs progressively from the origin to terminus within minutes of replication each locus. Between and segregation, sister loci are held in an apparent cohesive state by topological links. The decatenation activity topoisomerase IV (Topo IV) is required for replicated loci, yet little known about structuring maintained a state. this work, we investigated folding cells with altered activities. Within after Topo inactivation, massive reorganization...
Abstract Aberrant DNA replication is a major source of the mutations and chromosomal rearrangements associated with pathological disorders. In bacteria, several different lesions are repaired by homologous recombination, process that involves sister chromatid pairing. Previous work in Escherichia coli has demonstrated interactions (SCIs) mediated topological links termed precatenanes, controlled topoisomerase IV. present work, we demonstrate during repair mitomycin C-induced lesions, rapidly...
Abstract Gene context can have significant impact on gene expression but is currently not integrated in quantitative models of regulation despite known biophysical principles and vitro measurements. Conceptually, the simplest consists a single framed by two topological barriers, as twin transcriptional-loop model, which illustrates interplay between transcription DNA supercoiling. In vivo, supercoiling additionally modulated topoisomerases, whose modus operandi remains to be quantified....
Summary setB was identified as a high‐copy suppressor of the partition defect mutation in parC , encoding one subunits topoisomerase IV. Deletion this integral inner membrane protein causes delay chromosome segregation, whereas its overproduction nucleoid disintegration and stretching, leading to cell division defect. deletion mutants also exhibit synthetic phenotype when combined with mutations that delete C‐terminal motor domain septal ring FtsK. SetB localizes helix interacts MreB,...