A5102831191- CRISPR and Genetic Engineering
- Cancer Research and Treatments
- Evolutionary Game Theory and Cooperation
- Slime Mold and Myxomycetes Research
- Vibrio bacteria research studies
- Evolution and Genetic Dynamics
- Ecosystem dynamics and resilience
- Insect symbiosis and bacterial influences
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Innovation and Socioeconomic Development
- Bacterial biofilms and quorum sensing
- Comparative and International Law Studies
- Crystallization and Solubility Studies
- Malaria Research and Control
- Silk-based biomaterials and applications
- Vietnamese History and Culture Studies
- Biocrusts and Microbial Ecology
- Korean Peninsula Historical and Political Studies
- Gene Regulatory Network Analysis
- Advanced Fluorescence Microscopy Techniques
- Invertebrate Immune Response Mechanisms
- Manufacturing Process and Optimization
University of California, San Diego
2017-2025
Center for Systems Biology
2020
University of California, Los Angeles
2020
Australian Defence Force Academy
2020
Sightsavers
2015
Princeton University
2012
Williams College
2009
University of Southern California
1999
Children's Hospital of Los Angeles
1999
Foreign and Commonwealth Office
1991
Horizontal gene transfer (HGT) plays a major role in the spread of antibiotic resistance. Of particular concern are Acinetobacter baumannii bacteria, which recently emerged as global pathogens, with nosocomial mortality rates reaching 19–54% (Centers for Disease Control and Prevention, 2013; Joly Guillou, 2005; Talbot et al., 2006). gains resistance remarkably rapidly (Antunes 2014; 2005), multi drug-resistance (MDR) exceeding 60% Centers 2013). Despite growing 2006), mechanisms underlying...
Synthetic biology has developed sophisticated cellular biosensors to detect and respond human disease. However, have not yet been engineered specific extracellular DNA sequences mutations. Here, we naturally competent Acinetobacter baylyi donor from the genomes of colorectal cancer (CRC) cells, organoids, tumors. We characterized functionality in vitro with coculture assays then validated them vivo sensor bacteria delivered mice harboring observed horizontal gene transfer tumor our mouse...
Diverse interactions among species within bacterial colonies lead to intricate spatiotemporal dynamics, which can affect their growth and survival. Here, we describe the emergence of complex structures in a colony grown from mixtures motile non-motile on soft agar surface. Time-lapse imaging shows that bacteria 'hitchhike' as latter migrate outward. The accumulate at boundary trigger an instability leaves behind striking flower-like patterns. mechanism front governing this pattern formation...
Motile eukaryotic cells migrate with directional persistence by alternating left and right turns, even in the absence of external cues. For example, Dictyostelium discoideum crawl extending distinct pseudopods an right-left pattern. The mechanisms underlying this zig-zag behavior, however, remain unknown. Here we propose a new Excitable Cortex Memory (EC&M) model for understanding alternating, extension pseudopods. Incorporating elements previous models, consider cell cortex as excitable...
ABSTRACT The gastrointestinal (GI) tract is a site of replication severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and GI symptoms are often reported by patients. SARS-CoV-2 cell entry depends upon heparan sulfate (HS) proteoglycans, which commensal bacteria that bathe the human mucosa known to modify. To explore gut HS-modifying bacterial abundances how their presence may impact infection, we developed task-based analysis proteoglycan degradation on large-scale shotgun...
CRISPR-Cas systems are prokaryotic immune that have proliferated widely not only in bacteria and archaea, but also much more recently, human biological research applications. Much work to date has utilized synthetic sgRNAs along with the CRISPR nuclease Cas9, discovery of array-processing nucleases now allows use compact, natural arrays heterologous hosts, addition organisms endogenous systems. Unfortunately, construction multiplex remains technically challenging, expensive, and/or...
While most of the morphological features hair, both animal and human, can be distinguished by an experienced microscopist without preparation either transverse or longitudinal sections, it is some, times necessary usually desirable to observe hair at least in cross section. This becomes especially true when dealing with hairs, aspect which often highly diagnostic a family modifications genera. Many techniques have been described for this operation, but practice much difficulty encountered...
Bacteria in nature live complex communities with multiple cell types and spatially-dependent interactions. Studying cells well-mixed environments such as shaking culture tubes or flasks cannot capture these spatial dynamics, but growing full-fledged biofilms are difficult to observe real time. We present here a protocol for observing time-resolved, multi-species interactions at single-cell resolution. The involves bacterial near monolayer microfluidic device. As demonstration, we describe...
Summary Advances in bacterial engineering have catalysed the development of living cell diagnostics and therapeutics 1–3 , including microbes that respond to gut inflammation 4 intestinal bleeding 5 pathogens 6 hypoxic tumors 7 . Bacteria can access entire gastrointestinal tract 8 produce outputs measured stool or urine Cellular memory, such as bistable switches 4,9,10 genomic rearrangements 11 allows bacteria store information over time. However, biosensors not yet been engineered detect...
Abstract Diverse interactions among species within bacterial biofilms often lead to intricate spatiotemporal dynamics. The spatial structure of can determine growth and survival different species, but the mechanisms driving formation this are not fully understood. Here, we describe emergence complex structures in a biofilm grown from mixtures motile non-motile on soft agar surface. Time-lapse imaging shows that bacteria “hitchhike” as latter migrate outward. accumulate at boundary colony...
There is increasing evidence to show that the circumstances of people with visual impairment in UK have worsened recent years and statutory services are increasingly under pressure. As a voluntary sector provider, changes provision health social care meant an assessment new model service delivery needed be investigated. This article reviews changing landscape for sight loss, outlines findings current research into needs our client group discusses implications these findings, which ultimately...
Cell-free gene expression offers the power of biology without complications a living organism. Although many such systems exist, most are quite expensive to buy and/or require special equipment and finely honed expertise produce effectively. This protocol describes method bacterial cell-free lysate that supports high levels expression, using only standard laboratory requiring minimal processing. The uses an Escherichia coli strain producing endolysin does not affect growth, but which...
Abstract A source inversion technique for chemical constituents is presented that uses assimilated constituent observations rather than directly using the observations. The method tested with a simple model problem, which two-dimensional Fourier–Galerkin transport combined Kalman filter data assimilation. Inversion carried out Green’s function and are simulated from true state added Gaussian noise. forecast same spectral but differs by an unbiased error emissions models constant errors....
Abstract CRISPR-Cas systems are prokaryotic immune that have proliferated widely not only in bacteria and archaea, but also much more recently, human biological research applications. Much work to date has utilized synthetic sgRNAs along with the CRISPR nuclease Cas9, discovery of array-processing nucleases now allows use compact, natural arrays heterologous hosts, addition organisms endogenous systems. Unfortunately, construction multiplex remains technically challenging, expensive, and/or...
Sexual armaments are usually structures or other traits used during agonistic displays that evolve by intrasexual competition. The role of parasites in their evolution remains obscure. We investigated the relation infection three with behavior males Sceloporus occidentalis male contests staged lab. rivals' both and blue patch chroma best predicted aggression intensity focal males. Infections haemococcidians ticks also contributed to explain Lizards fewer engaged more intense fights, while...