A5083594296- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Bacterial biofilms and quorum sensing
- Cassava research and cyanide
- Antibiotic Resistance in Bacteria
- Pediatric Pain Management Techniques
- Anesthesia and Pain Management
- Aquatic Ecosystems and Biodiversity
- Anesthesia and Sedative Agents
- Child Nutrition and Water Access
- Salmonella and Campylobacter epidemiology
- Cephalopods and Marine Biology
United States Food and Drug Administration
2021
University of Pennsylvania
2017-2020
Pennsylvania State University
2016-2018
Bacterial pathogens are highly adaptable organisms, a quality that enables them to overcome changing hostile environments. For example, Vibrio cholerae, the causative agent of cholera, is able colonize host small intestines and combat host-produced reactive oxygen species (ROS) during infection. To dissect molecular mechanisms utilized by V. cholerae ROS in vivo, we performed whole-genome transposon sequencing analysis (Tn-seq) comparing gene requirements for colonization using adult mice...
Animal development and physiology depend on beneficial interactions with microbial symbionts. In many cases, the symbionts are horizontally transmitted among hosts, thereby making acquisition of these microbes from environment an important event within life history each host. The light organ symbiosis established between Hawaiian squid Euprymna scolopes bioluminescent bacterium Vibrio fischeri is a model system for examining how hosts acquire Recent studies have revealed that wild-caught E....
Vibrio cholerae is the causative agent of cholera, a dehydrating diarrheal disease. This Gram-negative pathogen able to modulate its gene expression in order combat stresses encountered both aquatic and host environments, including stress posed by reactive oxygen species (ROS). In further understanding V. cholerae's transcriptional response ROS, we performed an RNA sequencing analysis determine profile when exposed hydrogen hydroperoxide. Of 135 differentially expressed genes, VC0139 was...
Vibrio cholerae, the aetiological agent of cholera, possesses multiple iron acquisition systems, including those for transport siderophores. How these systems benefit V. cholerae in low-iron, polymicrobial communities environmental settings or during infection remains poorly understood. Here, we demonstrate that iron-limiting conditions, co-culture with a number individual siderophore-producing microbes significantly promoted growth vitro . We further show host environment low iron,...
Summary The fitness of host‐associated microbes depends on their ability to access nutrients in vivo . Identifying these mechanisms is significant for understanding how have evolved fill specific ecological niches within a host. Vibrio fischeri bioluminescent bacterium that colonizes and proliferates the light organ Hawaiian bobtail squid, which provides an opportunity study bacteria grow Here, transcription factor CysB shown be necessary V. both several sulfur sources vitro establish...
Like many other pathogens, Vibrio cholerae , the causative agent of cholera, can modulate its gene expression to combat stresses encountered in both aquatic and host environments, including stress posed by reactive oxygen species (ROS). We previously reported that virulence activator AphB V. is involved ROS resistance. In this study, we found another key regulator, ToxR, was important for resistance hydrogen peroxide.
Purpose Pain is a common side effect of intravenous injection propofol. We conducted randomized, prospective, single-blinded controlled trial to assess the efficacy vibration analgesia on pain during propofol infusion in ambulatory surgery. Methods After institutional review board approval, 100 patients undergoing elective surgery with general anesthesia were randomized into 2 groups. A control group (n = 50) consisted who received without analgesia. treatment using Buzzy device. was...