A5035816498- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Cystic Fibrosis Research Advances
- Asthma and respiratory diseases
- Regulation of Appetite and Obesity
- Fish Ecology and Management Studies
- Aquaculture disease management and microbiota
- Neonatal Respiratory Health Research
- Neurobiology and Insect Physiology Research
- Ocean Acidification Effects and Responses
- Fish biology, ecology, and behavior
- Biochemical Analysis and Sensing Techniques
- Reproductive biology and impacts on aquatic species
- Eosinophilic Esophagitis
- Receptor Mechanisms and Signaling
- Tracheal and airway disorders
- IL-33, ST2, and ILC Pathways
- Legume Nitrogen Fixing Symbiosis
- Pediatric health and respiratory diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Behavior and Reproduction
- melanin and skin pigmentation
- Coral and Marine Ecosystems Studies
- Respiratory and Cough-Related Research
- Crustacean biology and ecology
University of Massachusetts Amherst
2018-2025
United States Geological Survey
2018-2025
Eastern Ecological Science Center
2021-2025
Oklahoma State University
2023-2025
University of Denver
2022-2023
DePaul University
2015-2022
National Jewish Health
2020-2022
University of Colorado Denver
2020-2022
Denver School of Nursing
2022
Abstract Cystic fibrosis (CF) is caused by mutations in the cystic transmembrane conductance regulator (CFTR), which lead to early death due progressive lung disease. The development of small-molecule modulators that directly interact with CFTR aid protein folding (“correctors”) and/or increase channel function (“potentiators”) have proven be highly effective therapeutic treatment CF. Notably, incorporation next-generation corrector, elexacaftor, into a triple combination (marketed as...
All ionoregulating marine fishes examined to date utilize seawater-type ionocytes expressing the apical Cl- channel, cystic fibrosis transmembrane conductance regulator (Cftr) secrete Cl−. We performed transcriptomic, molecular, and functional studies identify Cl− transporters in of sea lamprey (Petromyzon marinus). Gill cftr expression was minimal or undetectable larvae post-metamorphic juveniles. identified other highly expressed gills and/or upregulated following metamorphosis further...
Chronic type 2 (T2) inflammatory diseases of the respiratory tract are characterized by mucus overproduction and disordered mucociliary function, which largely attributed to effects IL-13 on common epithelial cell types (mucus secretory ciliated cells). The role rare cells in airway T2 inflammation is less clear, though tuft have been shown be critical initiation immunity intestine. Using bulk single-cell RNA sequencing epithelium mouse modeling, we found that expanded programmed toward...
The hypothalamic-pituitary-interrenal (HPI) axis is a highly conserved neuroendocrine system in vertebrates, but many details of its physiological role jawless vertebrates remain unclear. Unlike the proopiomelanocortin (Pomc) gene jawed lampreys have two divergent melanocortin prohormones, proopiocortin (Poc) encoding adrenocorticotropic hormone (Acth) and promelanotropin (Pom) melanocyte-stimulate hormones (Msh-a Msh-b). This study investigated HPI sea lamprey (Petromyzon marinus) by...
Abstract It is unknown whether and how osmoregulation controlled by corticosteroid signaling in the phylogenetically basal vertebrate group Agnatha, including lampreys hagfishes. known that a truncated steroid biosynthetic pathway produces two predominant circulating corticosteroids, 11-deoxycortisol (S) 11-deoxycorticosterone (DOC). Furthermore, express only single, ancestral receptor (CR). Whether S and/or DOC interact with CR to control still unknown. We examined role of endogenous...
The present study provides molecular and functional characterization of Na+-K+-2Cl- cotransporter (NKCC1/Slc12a2) in the gills sea lamprey (Petromyzon marinus), most basal extant vertebrate with an osmoregulatory strategy. We report full-length peptide sequence for Na-K-Cl 1 (NKCC1), which we show groups strongly occupies a position among other NKCC1 sequences. In postmetamorphic juvenile lamprey, nkcc1 mRNA was many tissues but fivefold higher gill than any examined tissue, protein only...
Lampreys are the most basal vertebrates with an osmoregulatory strategy. Previous research has established that salinity tolerance of sea lamprey increases dramatically during metamorphosis, but underlying changes in gut have not been examined. In present work, we examined intestinal function metamorphosis and seawater exposure ( Petromyzon marinus). Fully metamorphosed juvenile had 100% survival after direct to 35 parts per thousand (SW) only slight elevations plasma chloride (Cl − )...
Abstract With remarkably few exceptions, aquatic vertebrates maintain internal Cl − homeostasis despite strong and sometimes fluctuating concentration gradients between extracellular fluids external environments. In this “Perspective,” we discuss recent advances in the understanding of epithelial transport at molecular level within key osmoregulatory organs fishes. New insights into mechanisms for basal lineages are highlighted to provide an evolutionary context. We describe processes that...
Although corticosteroid-mediated hepatic gluconeogenic activity in response to stress has been extensively studied fishes and other vertebrates, there is little information on the basal vertebrates. In sea lamprey (Petromyzon marinus), a representative member of most extant vertebrate group Agnatha, 11-deoxycortisol deoxycorticosterone are major circulating corticosteroids. The present study examined changes glucose concentrations physical stressor. Furthermore, actions were examined. Within...
Two orthologues of the gene encoding Na+-Cl- cotransporter (NCC), termed ncca and nccb, were found in sea lamprey genome. No Na+-K+-2Cl- 2 (nkcc2) was identified. In a phylogenetic comparison among other vertebrate NCC NKCC sequences, NCCs occupied basal positions within clades. freshwater, mRNA only gill nccb intestine, whereas both kidney. Intestinal levels increased during late metamorphosis coincident with salinity tolerance. Acclimation to seawater intestine Electrophysiological...
Cystic fibrosis (CF) is a genetic disease caused by mutations of the gene encoding cAMP-activated Cl- channel, cystic transmembrane conductance regulator (CFTR). CFTR modulator therapies consist small-molecule drugs that rescue mutant CFTR. Regimens single or combinations modulators still rely on endogenous levels cAMP to regulate activity. We investigated activation natural mediator prostaglandin E2 (PGE2) and lubiprostone (a Food Drug Administration-approved drug known target receptors)...
ABSTRACT Migratory fishes encounter a variety of environmental conditions, including changes in salinity, temperature and dissolved gases, it is important to understand how these are able acclimate multiple stressors. The gill the primary site both acid–base balance ion regulation fishes. Many transport mechanisms involved with compensation also required for plasma Na+ Cl+, predominant extracellular ions, potentially resulting strong interaction between ionoregulation regulation. present...
The aims of this study were to assess whether thermal tolerance brook trout (Salvelinus fontinalis) is affected during seawater (SW) acclimation and investigate the role cortisol in osmoregulation SW acclimation. Freshwater (FW)-acclimated at 18 °C (Tacc) exposed for 16 d, whilst maintaining a FW control. Fish examined critical maximum (CTmax) 0 (before), 2, 5, d after exposure, sampled Tacc CTmax analysis plasma cortisol, glucose, Cl−, gill Na+/K+-ATPase (NKA) activity heat shock protein 70...
To maintain internal ion balance in marine environments, teleost fishes leverage seawater (SW)-type ionocytes to actively secrete Na + and Cl - into the environment. It is well established that SW-type utilize apically expressed cystic fibrosis transmembrane conductance regulator 1 (Cftr1) as a conduit for exit gill. Here, we investigated whether Ca 2+ -activated channel, anoctamin (Ano1), provides an additional path -secretion euryhaline mummichogs ( Fundulus heteroclitus). Two ano1 gene...
Abstract Species range shifts due to climate alterations have been increasingly well‐documented. Although amphibians are one of the most sensitive groups animals environmental perturbations change, almost no studies offered evidence poleward distribution in this taxon response warming. Range would be facilitated by variation traits associated with ability species persist and/or shift their face but extent and consequences intraspecific these is unclear. We studied role ongoing green...
Introduction One method for assessing the in vitro response to CFTR-modulating compounds is by analysis of epithelial monolayers an Ussing chamber, where apical and basolateral surfaces are isolated potential difference, short-circuit current, transepithelial resistance can be monitored. The effect a chloride ion gradient across airway epithelia on transport magnitude CFTR modulator efficacy were examined. Methods CFTR-mediated changes difference currents primary human nasal cell cultures...
Human melanocortin-2 receptor (hMC2R) co-expressed with the accessory protein mouse (m)MRAP1 in Chinese Hamster Ovary (CHO) cells has been used as a model system to investigate activation and trafficking of hMC2R. A previous study had shown that N-terminal domain mMRAP1 makes contact one extracellular domains hMC2R facilitate chimeric paradigm was which were replaced corresponding from Xenopus tropicalis MC1R, does not interact MRAP1, reveal EC2 (Extracellular 2) is most likely site for...
In derived bony vertebrates, activation of the melanocortin-2 receptor (Mc2r) by its ACTH ligand requires chaperoning Mc2r accessory protein (Mrap1). The N-terminal domain non-mammalian tetrapod MRAP1 from chicken (c; Gallus gallus) has putative motif, W18D19Y20I21, and in neopterygian ray-finned fish Mrap1 bowfin (bf; Amia calva) Y18D19Y20I21. current study used an alanine-substitution paradigm to test hypothesis that only Y20 position ortholog these vertebrates is required for respective...