A5061766234- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Radiation Therapy and Dosimetry
- Gene expression and cancer classification
- Plant Taxonomy and Phylogenetics
- Plant Stress Responses and Tolerance
- Genetics and Plant Breeding
- Carcinogens and Genotoxicity Assessment
- Photosynthetic Processes and Mechanisms
- Tree Root and Stability Studies
Cornell University
2018-2023
Brigham Young University
2018
West Virginia University
2018
University of California, Davis
2014-2017
Institute of Genetics
2009
The Arabidopsis sog1-1 (suppressor of gamma response) mutant was originally isolated as a second-site suppressor the radiosensitive phenotype seeds defective in repair endonuclease XPF. Here, we report that SOG1 encodes putative transcription factor. This gene is member NAC domain [petunia NAM (no apical meristem) and ATAF1, 2 CUC2] family (a proteins unique to land plants). Hundreds genes are normally up-regulated within an hour treatment with ionizing radiation; induction these requires...
Abstract Whereas our knowledge about the diverse pathways aiding DNA repair upon genome damage is steadily increasing, little known molecular players that adjust plant cell cycle in response to stress. By a meta-analysis of stress microarray data sets, three family members SIAMESE/SIAMESE-RELATED (SIM/SMR) class cyclin-dependent kinase inhibitors were discovered react strongly genotoxicity. Transcriptional reporter constructs corroborated specific and strong activation SIM/SMR genes...
Grass leaves develop from a ring of primordial initial cells within the periphery shoot apical meristem, pool organogenic stem that generates all organs plant shoot. At maturity, grass leaf is flattened, strap-like organ comprising proximal supportive sheath surrounding and distal photosynthetic blade. The blade are partitioned by hinge-like auricle ligule, fringe epidermally derived tissue grows adaxial (top) surface. Together, ligule comprise morphological novelties specific to leaves....
Abstract Forward genetics remains a powerful method for revealing the genes underpinning organismal form and function, how these are tied together in gene networks. In maize, forward has been tremendously successful, but size complexity of maize genome made identifying mutant an often arduous process with traditional methods. The next generation sequencing revolution allowed cloning to be significantly accelerated many organisms, even when genomes large complex. Here, we describe...
In Arabidopsis, DNA damage-induced programmed cell death is limited to the meristematic stem niche and its early descendants. The significance of this cell-type-specific unclear. Here, we demonstrate in roots that it destruction mitotically compromised triggers regeneration, enabling growth recovery. contrast wild-type plants, sog1 which are defective death, maintain identities stereotypical structure after irradiation, but these cells fail undergo division, terminating root growth. We...
The mechanisms whereby leaf anlagen undergo proliferative growth and expansion to form wide, flat leaves are unclear. maize gene NARROWSHEATH1 (NS1) is a WUSCHEL-related homeobox3 (WOX3) homolog expressed at the margins of primordia, required for mediolateral outgrowth. To investigate NS1 function, we used chromatin immunoprecipitation laser-microdissection RNA-seq primordial identify targets bound modulated by NS1. Microscopic analyses cell division expression in expanding leaves, reverse...
Plants exhibit a robust transcriptional response to gamma radiation which includes the induction of transcripts required for homologous recombination and suppression that promote cell cycle progression. Various DNA damaging agents induce different spectra damage as well "collateral" other cellular components therefore are not expected provoke identical responses by cell. Here we study effects two types ionizing (IR) treatment, HZE (1 GeV Fe(26+) high mass, charge, energy relativistic...
Low linear energy transfer (LET) gamma rays and high LET HZE (high atomic weight, energy) particles act as powerful mutagens in both plants animals. DNA damage generated by is more densely clustered than that rays. To understand the genetic requirements for resistance to vs. low radiation, a series of Arabidopsis thaliana mutants were exposed either 1GeV Fe nuclei or radiation. A comparison effects on germination subsequent growth seedlings led us conclude relative biological effectiveness...
Forward genetics remains a powerful method for revealing the genes underpinning organismal form and function, how these are tied together in gene networks. In maize, forward has been tremendously successful, but size complexity of maize genome made identifying mutant an often arduous process with traditional methods. The next generation sequencing revolution allowed cloning to be significantly accelerated many organisms, even when genomes large complex. Here, we describe bulked-segregant...
In the past, identifying a gene of interest for mutant phenotype in maize has proven to be laborious process. Now, with aid next generation sequencing technology, researcher can quickly identify region containing and some cases causative lesion. our BSA-seq protocol, we use Galaxy platform file processing manipulation. This is intended provide user friendly experience, so that researchers any level clone genes. We start raw reads from F2 mapping population end plots SNPs on each chromosome...