Abstract ELONGATED HYPOCOTYL5 (HY5) is a basic domain/leucine zipper (bZIP) transcription factor, central for the regulation of seedling photomorphogenesis. Here, we identified B-BOX (BBX)–containing protein, BBX25/SALT TOLERANCE HOMOLOG, as an interacting partner HY5, which has been previously found to physically interact with CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). BBX25 interacts HY5 both in vitro and vivo. By physiological genetic approaches, showed that negative regulator its homolog...

Abstract The crosstalk of light signaling pathways with other cascades has just started to be revealed. Here, we report the identification and functional characterization a Z-box binding factor (ZBF1) in pathways. Arabidopsis thaliana ZBF1 encodes AtMYC2/JIN1, basic helix-loop-helix transcription factor, which recently been shown involved abscisic acid (ABA), jasmonic (JA), jasmonate-ethylene We demonstrate that AtMYC2 interacts Z- G-box light-responsive elements minimal light–regulated...

Light is one of the most important environmental cues regulating multiple aspects plant growth and development, abscisic acid (ABA) a hormone that plays roles during many phases life cycle in plants' responses to various stresses. How plants integrate external light signal with endogenous ABA pathway for better adaptation survival remains poorly understood. Here, we show BBX21 (also known as SALT TOLERANCE HOMOLOG 2), B-box (BBX) protein previously shown positively regulate seedling...

Abstract Arabidopsis thaliana CALMODULIN7 (CAM7), a unique member of the calmodulin gene family, plays crucial role as transcriptional regulator in seedling development. The elongated HYPOCOTYL5 (HY5) bZIP protein, an integrator multiple signaling pathways, also important photomorphogenic growth and light-regulated expression. CAM7 acts synergistically with HY5 to promote photomorphogenesis at various wavelengths light. Although genetic relationships between light-mediated development have...

Plant growth and development are defined by environmental cues. The transcription factor PHYTOCHROME INTERACTING FACTOR 4 (PIF4) is the central signaling hub that integrates cues, including light temperature, to regulate development. thermosensory mechanisms controlling PIF4-mediated temperature response, its integration with other responses, remain poorly understood. DE-ETIOLATED 1 (DET1) CONSTITUTIVE PHOTOMORPHOGENESIS (COP1), key regulators of signaling, have been proposed control...

Temperature is a key seasonal signal that shapes plant growth. Elevated ambient temperature accelerates growth and developmental transitions [1] while compromising defenses, leading to increased susceptibility [2, 3]. Suppression of immunity at elevated the interface trade-off between defense 4]. Climate change increase in average growth-season temperatures threaten biodiversity food security [5, 6]. Despite its significance, molecular mechanisms link thermosensory responses are not known....

MYC2 is a basic helix-loop-helix transcription factor that cross talks with light, abscisic acid (ABA), and jasmonic (JA) signaling pathways. Here, we have shown Arabidopsis (Arabidopsis thaliana) directly binds to the G-box present in SUPPRESSOR OF PHYTOCHROME A1 (SPA1) promoter it controls expression of SPA1 COP1-dependent manner. Analyses atmyc2 spa1 double mutants suggest whereas act redundantly suppress photomorphogenic growth dark, they function synergistically for suppression light....

BBX24 and BBX25 are two important transcriptional regulators, which regulate seedling photomorphogenesis in Arabidopsis. Very recently, we have shown that negatively the expression of BBX22, reducing function HY5, by physically interacting with its bZIP domain. (1) Furthermore, HY5 HOMOLOG, HYH, has been reported to heterodimerize enhances photomorphogenic de-etiolation serving as coactivator. (8) Here, further report interact HYH. The physical interactions HYH could lead depletion molecules...

Summary Arabidopsis MYC 2 bHLH transcription factor plays a negative regulatory role in blue light (BL)‐mediated seedling development. HY 5 bZIP protein works as positive regulator of multiple wavelengths and promotes photomorphogenesis. Both 5, belonging to two different classes factors, are the integrators signaling pathways. However, functional interrelations these factors development remain unknown. Additionally, whereas 5‐mediated regulation gene expression has been investigated detail,...

ABSTRACT Light is one of the most critical ecological cues controlling plant growth and development. Plants have evolved complex mechanisms to cope with fluctuating light signals. In Arabidopsis, bHLH transcription factors MYC2, MYC3, MYC4 been shown play a vital role in protecting plants against herbivory necrotrophic pathogens. While MYC2 light‐mediated seedling development has studied some detail, MYC3 still needs be discovered. Here, we show that negatively regulates photomorphogenesis,...

SUMMARY COP1 is the essential protein that integrates various environmental and hormonal cues to control plant growth development at multiple levels. a RING‐finger‐type E3 ubiquitin ligase acts as potent repressor of photomorphogenesis flowering by targeting numerous substrates for ubiquitination promoting their proteolysis via 26S proteasome system. The WD40 repeat domain with conserved amino acid residues was shown be interacting its targets. However, role these acids in regulating...

Regulations of Arabidopsis seedling growth by two proteins, which belong to different classes transcription factors, are poorly understood. MYC2 and GBF1 bHLH bZIP respectively, function in cryptochrome-mediated blue light signaling. Here, we have investigated the molecular functional interrelation light-mediated photomorphogenesis. Our study reveals that colocalize physically interact nucleus. This interaction requires N-terminal domain each protein. The atmyc2 gbf1 double mutant analyses...

Although many transcription factors and regulatory proteins have been identified functionally characterized in light signaling pathways, photoperception to remains largely fragmented. The Z-box is one of the LREs (Light responsive elements) that plays important role regulation during light-controlled Arabidopsis seedling development. involvement photoreceptors modulation activity containing promoters has demonstrated. However, downstream components such as SPA1 MYC2/ZBF1, which are...

Arabidopsis (Arabidopsis thaliana) Short Hypocotyl in White Light1 (SHW1) encodes a Ser-Arg-Asp-rich protein that acts as negative regulator of photomorphogenesis. SHW1 and Constitutive Photomorphogenic1 (COP1) genetically interact an additive manner to suppress Elongated Hypocotyl5 (HY5) is photomorphogenesis promoting basic leucine zipper transcription factor degraded by COP1 ubiquitin ligase the darkness. Here, we report functional interrelation with HY5 seedling development. The vitro...

Plant growth and development outcomes of plant-microbe interactions are defined by coordinated responses to seasonal signals. The mechanisms that control the regulation immunity not well understood. Here, we show a common signaling module integrates environmental signals, such as photoperiod temperature, regulate growth-defense balance. Key light-signaling components De-Etiolated 1 (DET1) Constitutive Photomorphogenic (COP1) negatively essential for immune modulation temperature. Our results...

MYC2 and SPA1 are two key regulatory proteins that negatively regulate light-controlled Arabidopsis seedling development.1,2 We have recently demonstrated the genetic molecular relationships of in light JA (jasmonic acid) signaling pathways.3 Here, we further shown interactions between these flowering time lateral root development.

Abstract Light is an important factor for plant growth and development. We have identified functionally characterized a regulatory gene SHORT HYPOCOTYL IN WHITE LIGHT1 (SHW1) involved in Arabidopsis (Arabidopsis thaliana) seedling SHW1 encodes unique serine-arginine-aspartate-rich protein, which constitutively localized the nucleus of hypocotyl cells. Transgenic analyses revealed that expression developmentally regulated closely associated with photosynthetically active tissues. Genetic...

MYC2 is an important transcription factor, which modulates by directly binding to Z-, G- and E-box elements present in the promoters of light different stress responsive genes.Citation1–Citation3 Very recently, we have shown that plays a role regulation Z- and/or G-box containing during both seedling adult plant growth.Citation4 Although, does not bind GATA box element (LRE) vitro as DNA assaysCitation1, its involvement regualtion GATA-box promoter planta, if any, known. Here, report...

The unique member of the calmodulin gene family, Calmodulin7 (CAM7), plays a crucial role as transcriptional regulator to promote Arabidopsis seedling development. CAM7 regulates expression HY5, which is intimately involved in promotion photomorphogenic growth and light-regulated expression. COP1 ubiquitin ligase suppresses photomorphogenesis by degrading multiple promoting factors including HY5 darkness. Genetic interaction studies, this report, reveal that co-ordinately work at lower...

Summary Light is one of the most critical ecological cues controlling plant growth and development. Plants have evolved complex mechanisms to cope with fluctuating light signals. In Arabidopsis, bHLH transcription factors MYC2, MYC3, MYC4 been shown play a vital role in protecting plants against herbivory necrotrophic pathogens. While MYC2 light-mediated seedling development has studied some detail, MYC3 still needs be discovered. Here, we show that negatively regulates photomorphogenesis,...